EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.3.1.47 | (R)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine |
8.6% product is formed matching the portion of S-enantiomer substrate (9.3%),37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 10 microM (R)-8-amino-7-oxononanoic acid |
Mycobacterium tuberculosis |
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid) |
- |
? |
2.3.1.47 | (S)-8-amino-7-oxononanoic acid + (R)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine |
46.3% product is formed from the racemic substrate, 37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM racemic 8-amino-7-oxononanoic acid |
Mycobacterium tuberculosis |
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid) |
- |
? |
2.3.1.47 | (S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine |
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM (S)-8-amino-7-oxononanoic acid |
Mycobacterium tuberculosis |
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid) |
- |
? |
2.3.1.47 | (S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine |
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, varying S-adenosyl-L-methionine, 9 microM (S)-8-amino-7-oxononanoic acid, varying inhibitor concentration |
Escherichia coli |
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid) |
- |
? |
2.3.1.47 | (S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine |
93.2% product is formed matching the portion of S-enantiomer substrate (94.6%), 37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, 1 mM S-adenosyl-L-methionine, 2 microM (S)-8-amino-7-oxononanoic acid |
Mycobacterium tuberculosis |
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid) |
- |
? |
2.3.1.47 | (S)-8-amino-7-oxononanoic acid + S-adenosyl-L-methionine |
37°C, direct assay with 100 mM N-(2-hydroxyethyl)piperazine-N'-(2-propanesulfonic acid) buffer (pH 8.6), 0.1 mM pyridoxal 5'-phosphate, varying S-adenosyl-L-methionine, 9 microM (S)-8-amino-7-oxononanoic acid, varying inhibitor concentration |
Escherichia coli C268 |
(7R,8S)-diaminopelargonic acid + S-adenosyl-(4-methylthio-2-oxobutanoic acid) |
- |
? |
2.3.1.47 | 6-carboxyhexanoyl-CoA + D-alanine |
the L-isomer of the amino acid is preferred, D-alanine shows 5fold slower reaction, interaction analysis, overview |
Mycobacterium tuberculosis |
8-amino-7-oxononanoate + CoA + CO2 |
i.e. D-KAPA |
? |
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine |
- |
Escherichia coli |
8-amino-7-oxononanoate + CoA + CO2 |
- |
? |
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine |
absolutely specific for L-alanine |
Lysinibacillus sphaericus |
8-amino-7-oxononanoate + CoA + CO2 |
i.e. 7-oxo-8-amino-pelargonic acid |
? |
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine |
i.e. pimeloyl-CoA |
Alcaligenes faecalis |
8-amino-7-oxononanoate + CoA + CO2 |
i.e. 7-oxo-8-amino-pelargonic acid |
? |