EC Number   |
|---|
   2.7.7.49 | - |
| 2.7.7.49 | chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol and containing the 5'-3' nuclease domain from Thermus sp. Z05 DNA polymerase (residues 1-291) and the 3'-5' exonuclease and polymerase domains from Thermotoga maritima DNA polymerase (residues 292-893). This chimera retains thermostable DNA polymerase activity, as well as proofreading activity. Using the CS5 chimera, a series of mutant proteins is constructed in which the amino acid side chains are mutated to modulate the 3'-5' exonuclease activity. The chimeric DNA polymerases are overexpressed under the control of the lambda PL promoter are expressed in Escherichia coli |
| 2.7.7.49 | cloned from a new HIV-1 group O isolate from Spain and expressed in Escherichia coli |
| 2.7.7.49 | construction of a gene fusion expressing stable fusion protein, expression in Escherichia coli. The resulting gene fusion consists of an open reading frame encoding 698 amino acids. The first 18 amino acids at the N terminus are encoded by the trpE gene, followed by 7 amino acids which are encoded by the pol gene but are not part of the reverse transcriptase. The subsequent 664 amino acids are encoded by the pol gene and the terminal 9 amino acids by pBR322. Construction of deletions at the 3' terminus of the gene results in a 4fold increase in the level of the reverse transcriptase activity in the soluble fraction of crude lysates |
| 2.7.7.49 | construction of a plasmid that induces in bacteria the synthesis of an enzymatically active reverse transcriptase, expression of a protein with a six-histidine tag in Escherichia coli |
| 2.7.7.49 | diverse parts of the sequence coding for reverse-transcriptase are subcloned and expressed in Escherichia coli |
| 2.7.7.49 | ectopic expression in the heterohybridoma cell line K6H6/B5 |
| 2.7.7.49 | expressed in Escherichia coli BL21 cells |
| 2.7.7.49 | expressed in Escherichia coli strain Bli5 |
| 2.7.7.49 | expression in Escherichia coli |
