EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.8.1.4 | -999 |
- |
- |
392950 |
| 1.8.1.4 | -999 |
- |
in thepresence of 2 M KCI, the thermal stability properties of mutant enzymes E423S, E423Q and E423A are comparable to those of wild-type, although the mutants appear to be slightly more stable. Mutant enzyme E423D however, is inactivated at temperatures around 20°C lower than for the wild-type. In the absence of salt, similar relative thermostabilities are observed, but both wild-type and mutant enzymes are inactivated at temperatures around 16-18°C lower than in the presence of salt |
727409 |
| 1.8.1.4 | 4 |
- |
activity is completely lost after 1 month |
738999 |
| 1.8.1.4 | 40 |
- |
recombinant enzyme is stable at 30°C upon 30 min of incubation without substrates, but incubation at 40°C and higher temperatures inactivated enzyme activity |
702739 |
| 1.8.1.4 | 55 |
70 |
the remaining activity after thermal treatment at pH 7.0 for 15 min is 90% at 55°C, 80% at 60°C, 70% at 65 °C, and 45% at 70°C |
712521 |
| 1.8.1.4 | 60 |
- |
the enzyme maintains some activity after a 30-min incubation |
691392 |
| 1.8.1.4 | 65 |
- |
170 min, 50% loss of activity |
393990 |
| 1.8.1.4 | 65 |
- |
t1/2: 170 min |
738999 |
| 1.8.1.4 | 65 |
- |
the enzyme unfolds irreversibly at heat treatment higher than 65°C |
674368 |
| 1.8.1.4 | 70 |
- |
50 min, 50% loss of activity |
393990 |
