EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Reference |
---|
2.7.4.22 | -999 |
- |
below 6.5 with 2 mM ATP, in the presence of MgCl2 |
663945 |
2.7.4.22 | -999 |
- |
below 6.5 with 2 mM ATP, in the presence of MnCl2 |
663945 |
2.7.4.22 | 6.5 |
- |
with 4 mM ATP, in the presence of MgCl2 |
663945 |
2.7.4.22 | 6.8 |
- |
- |
692284 |
2.7.4.22 | 6.8 |
- |
purified recombinant enzyme |
692284 |
2.7.4.22 | 7 |
- |
pH variation influences only turnover rate, at 60°C |
672233 |
2.7.4.22 | 7 |
- |
variation of pH only influences the turnover number, at 60°C, 50 mM succinate/phosphate buffer, phosphate buffer, glycine buffer, assays are performed with 100 microM UMP, 500 microM ATP, and 10 mM MgCl2 |
672233 |
2.7.4.22 | 7.1 |
- |
with 4 mM ATP, in the presence of MnCl2 |
663945 |
2.7.4.22 | 7.4 |
- |
assay at |
698858 |
2.7.4.22 | 7.4 |
- |
when Mn2+ serves as the cofactor, maximal enzyme activity is found at pH 7.4 |
721458 |