1.2.1.80 | ligand-free enzyme and three complex structures in which the enzyme binds to aldehyde-deformylating oxygenase and various ligands (stearoyl-CoA, NADPH, thioester), hanging drop vapor diffusion method, using 0.1 M citric acid, pH6.0, and 1 M (NH4)2SO4 (ligand-free enzyme) or 0.1 M HEPES, pH 7.5, and 20% (w/v) PEG8000 (enzyme in complex with ligands) |