EC Number   |
Protein Variants |
Reference |
|---|
   1.14.12.3 | A291S |
reduced activity with ethylbenzene |
657604 |
| 1.14.12.3 | E444D |
no effect on activity |
657604 |
| 1.14.12.3 | G404D |
reduced activity with ethylbenzene |
657604 |
| 1.14.12.3 | H119C |
the mutant alpha-subunit of the terminal dioxygenase is unable to coordinate an EPR-detectable Rieske [2Fe-2S] cluster with the characteristic g factors |
-, 439070 |
| 1.14.12.3 | H222M |
in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit is unable to reconstitute dioxygenase activity |
-, 439070 |
| 1.14.12.3 | H228C |
in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit is unable to reconstitute dioxygenase activity |
439070 |
| 1.14.12.3 | H98C |
the mutant alpha-subunit of the terminal dioxygenase is unable to coordinate an EPR-detectable Rieske [2Fe-2S] cluster with the characteristic g factors, detection of a novel EPR spectrum, the intensity of the spectrum is approximately 8% from the wild-type |
-, 439070 |
| 1.14.12.3 | I301V |
the mutation in the C-terminal part of subunit alpha enhances the substrate specificity for ethylbenzene, the mutant shows altered patterns of products formed from toluene and ethylbenzene, including monohydroxylated side chains |
657604 |
| 1.14.12.3 | I301V/T305S/I307L/L309V |
increased activity with ethylbenzene |
657604 |
| 1.14.12.3 | I301V/T305S/I307L/L309V |
the mutations in the C-terminal part of subunit alpha enhance the substrate specificity for ethylbenzene, the quadruple mutant also shows a high uncoupled rate of electron transfer without product formation |
657604 |
