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EC Number
Protein Variants
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1.2.1.65
E175A
site-directed mutagenesis, the apparent Km value of the mutant enzyme to NAD+ is increased by about 2times and the kcat/Km toward the cofactor decreased by 4times. The Km value of the mutant toward salicylaldehyde also increases and the catalytic efficiency decreases by 6times compared to the wild-type enzyme
743833
1.2.1.65
more
site-directed mutagenesis of selected residues binding NAD+ and/or SAL affects the enzyme's catalytic efficiency, but does not eliminate catalysis
743833
1.2.1.65
N149A
site-directed mutagenesis, the catalyic efficiency of the mutant is decreased compared to the wild-type enzyme
743833
1.2.1.65
V153A
site-directed mutagenesis, the mutation has no significant effect on the kinetic parameters for either NAD+ or salicylaldehyde compared to the wild-type enzyme
743833
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