EC Number |
Protein Variants |
Reference |
---|
1.3.98.7 | C251A |
insoluble |
760607 |
1.3.98.7 | C269A |
auxiliary [4Fe-4S] cluster I mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo but incapable of converting MftA to MftA* or MftA** |
760607 |
1.3.98.7 | C269A/C323A |
insoluble |
760607 |
1.3.98.7 | C30A/C34A/C37A |
insoluble |
760607 |
1.3.98.7 | C30A/C37A |
radical S-adenosylmethionine mutant, can neither cleave SAM nor modify MftA |
760607 |
1.3.98.7 | C310A/C341A |
auxiliary [4Fe-4S] cluster II mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo, incapable of converting MftA to MftA* or MftA** |
760607 |
1.3.98.7 | C323A |
auxiliary [4Fe-4S] cluster I mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo, incapable of converting MftA to MftA* or MftA** |
760607 |
1.3.98.7 | more |
systematical replacement of Cys residues by Ala. The RS KO could neither cleave SAM nor modify MftA, consistent with the successful knockout of the RS cluster. Activity assays for Aux I and Aux II KO's also provided insightful results. Both Aux I and Aux II KO's were capable of catalyzing the reductive cleavage of SAM to form dAdo (Figure 3A), suggesting that the RS cluster remained intact and in an active conformation in the mutated proteins. However, when assayed against MftA, both Aux I and Aux II KO's were incapable of converting MftA to MftA* or MftA** |
760607 |