EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.49 | A114G |
mutant enzyme retains significant DNA polymerase activity. 5-6fold increase in the ability of the enzyme to discriminate against ddNTPs |
660996 |
| 2.7.7.49 | A114S |
mutant enzyme retains significant DNA polymerase activity, enzyme shows wild-type ddNTP/dNTP discrimination efficiency |
660996 |
| 2.7.7.49 | A114T |
mutant enzyme shows very low catalytic efficiency in nucleotide incorporation assays, due to the high KM-values for dNTP. Decreased susceptibility to AZTTP when poly(rA)/(dT)16 is used as substrate |
660996 |
| 2.7.7.49 | A114V |
mutant enzyme shows very low catalytic efficiency in nucleotide incorporation assays, due to the high KM-values for dNTP. Decreased susceptibility to AZTTP when poly(rA)/(dT)16 is used as substrate, enzyme shows wild-type ddNTP/dNTP discrimination efficiency |
660996 |
| 2.7.7.49 | A502V |
site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme |
723690 |
| 2.7.7.49 | A608T/E520G/W827R |
substitution rates per base for RNA-dependent DNA is similar or lower than that of avian myeloblastosis virus reverse transcriptase. Rate constants kcat of about two orders of magnitude larger than those of the Stoffel fragment |
671330 |
| 2.7.7.49 | A644V |
site-directed mutagenesis, the mutant shows decreased thermostability and -performance compared to the wild-type enzyme |
723690 |
| 2.7.7.49 | C232A |
mutation in CP2 motif, modest defect in RNA binding |
738587 |
| 2.7.7.49 | D200N |
site-directed mutagenesis, the mutant shows increased thermostability and -performance compared to the wild-type enzyme |
723690 |
| 2.7.7.49 | D200N/L603W/T330P/L139P/E607K |
site-directed mutagenesis, highly processive and thermostable multiply-mutated M-MuLV RT variant with 65fold improvement in comparison to the wild-type enzyme, the maximum temperature of the full-length cDNA synthesis is raised to 62°C, compared to 45°C for the wild-type enzyme |
723690 |
