EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.4 | A171S/Y172S/G173S/N174F/E175I/E180M/G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y |
contrary to wild-type, mutant may be activated by Na+, mutations in 170 loop increase susceptibility to Na+ further |
667718 |
| 3.4.21.4 | C191A |
mutation decreases the activity of trypsin by 20-200fold as measured by the ratio of turnover number to Km-value for the hydrolysis of amide substrates, ester hydrolysis is decreased by less than 10fold |
95445 |
| 3.4.21.4 | C191A/C220A |
the mutant enzyme binds bovine pancreatic trypsin inhibitor with the same affinity as trypsin, the affinity of benzamidine is decresed 10fold and the affinity of leupeptin is decreased 100fold |
95445 |
| 3.4.21.4 | C220A |
mutation decreases the activity of trypsin by 20-200fold as measured by the ratio of turnover number to Km-value for the hydrolysis of amide substrates, ester hydrolysis is decreased by less than 10fold |
95445 |
| 3.4.21.4 | D189S/DELTA223 |
double mutant trypsinogen, the recombinant proteinase lacks trypsin-like activity but aquires a rather unique selectivity, it preferentially hydrolyses peptide bonds C-terminal to tyrosyl residues. This narrow specificity should be useful in peptide-analytical applications such as sequence-specific fragmentation of large proteins prior to sequencing |
649322 |
| 3.4.21.4 | E97N/Y99L |
Km value for Pefachrome is 1.5fold higher than the Km-value of the wild-type enzyme. The KI-value for benzamidine is 92% of the wild-type value, the KI-value for [4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl]-(3,4,5-6-tetrahydro-[2H-1,4']bipyridinyl-4yl)-methanone is 17% of the wild-type value, the KI-value for 2,7-bis(4-amidinobenzylidene)cycloheptan-1-one is 1.75fold higher than the wild-type value |
650468 |
| 3.4.21.4 | E97N/Y99L/S190A |
Km value for Pefachrome is 3.7fold higher than the Km-value of the wild-type enzyme. The KI-value for benzamidine is 1.3fold higher than wild-type value, the KI-value for [4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl]-(3,4,5-6-tetrahydro-[2H-1,4']bipyridinyl-4yl)-methanone is 6.7% of the wild-type value, the KI-value for 2,7-bis(4-amidinobenzylidene)cycloheptan-1-one is 2.25fold higher than the wild-type value |
650468 |
| 3.4.21.4 | G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y |
twofold increase in KM-value |
667718 |
| 3.4.21.4 | K188F |
increase of Km for Arg and Lys containing substrates, cleavage of nearly 30 new peptide bonds in beta-casein compared to the wild-type enzyme |
95457 |
| 3.4.21.4 | K188W |
increase of Km for Arg and Lys containing substrates, 3-4fold decrease in turnover number |
95457 |
