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1.1.1.357: 3alpha-hydroxysteroid 3-dehydrogenase

This is an abbreviated version!
For detailed information about 3alpha-hydroxysteroid 3-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.357

Reaction

a 3alpha-hydroxysteroid
+
NAD(P)+
=
a 3-oxosteroid
+
NAD(P)H
+
H+

Synonyms

17beta-HSD5, 3-alpha hydroxysteroid dehydrogenase type 3, 3-alpha-HSD type 2, 3-alpha-HSD1, 3-alpha-hydroxysteroid dehydrogenase type 2, 3-alpha-hydroxysteroid dehydrogenase type I, 3alpha-HSD, 3alpha-HSD type III, 3alpha-HSD/CR, 3alpha-HSD3, 3alpha-HSOR, 3alpha-hydroxysteroid dehydrogenase, 3alpha-hydroxysteroid dehydrogenase type 3, 3alpha-hydroxysteroid dehydrogenase type III, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, 3alpha-hydroxysteroid oxidoreductase, AKR1C1, AKR1C14, AKR1C2, AKR1C3, AKR1C33, AKR1C4, AKR1C9, aldo-keto reductase family 1 member C1, aldo-keto reductase family 1 member C2, aldo-keto reductase family 1 member C3, bile acid binding protein, chlordecone reductase, DD2, DD4, dihydrodiol dehydrogenase, dihydrodiol dehydrogenase 4, dihydrodiol/3alpha-hydroxysteroid dehydrogenase, HSD 28, HSD 29, hsdA, More, NADPH-dependent AKR1C9, Naloxone reductase 2, Ps3alphaHSD, type 1 3alpha-HSD, type 3 3alpha-hydroxysteroid dehydrogenase, type 5 17beta-hydroxysteroid dehydrogenase, type I 3alpha-HSD, type III 3-alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.357 3alpha-hydroxysteroid 3-dehydrogenase

Cloned

Cloned on EC 1.1.1.357 - 3alpha-hydroxysteroid 3-dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3alpha-HSD/CR gene cloned into pET15b vector with an N-terminal His tag sequence and transformation of Escherichia coli BL21(DE3) pLysS cells
AKR1C1, transient expression in COS-1 cells, and recombinant overexpression in Escherichia coli strain BL21(DE3), quantitative real-time PCR analysis
AKR1C2, transient expression in COS-1 cells, and recombinant overexpression in Escherichia coli strain BL21(DE3), quantitative real-time PCR analysis
AKR1C3, transient expression in COS-1 cells, and recombinant overexpression in Escherichia coli strain BL21(DE3), quantitative real-time PCR analysis
AKR1C4, transient expression in COS-1 cells, and recombinant overexpression in Escherichia coli strain BL21(DE3), quantitative real-time PCR analysis
expressed in COS-1 cells, PC-3 cells, and LNCaP cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli cells
-
expressed in Escherichia coli JM109 cells
-
expressed in Huh-7 and Hep-G2 cells
-
gene Akr1c14, recombinant expression in COS-1 cells
-
gene Akr1c9, recombinant expression in COS-1 cells
gene hsdA, recombinant expression of His-tagged and untagged enzyme in Escherchia coli strain BL21(DE3)
overexpression in Escherichia coli
overexpression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
recombinant expression in mouse PCa cells and of FLAG-AKR1C3 in mouse Rv1 cells, recombinant expression of His-tagged AKR1C3 in Escherichia coli strain BL21
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3). The N-terminal His-tag has little effect on the enzyme function
-
recombinant expression of wild-type and mutant enzymes
recombinant His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3)
sequence comparison and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)