the enzyme is extremely thermostable, and the activity of the pyrroloquinoline quinone-bound holoenzyme is not lost after incubation at 100 °C for 10 min, while the apoenzyme retains full activity at up to 80°C under the same conditions
100% activity at 25-30°C, about 90% activity at 35°C, about 80% activity at 40-45°C, about 750% activity at 50°C, about 60% activity at 55°C, after incubation at 60°C for 1 h the enzyme still has 27% of its initial activity while loosing nearly all activity after treatment at 65°C
low temperature of below 40°C has the stable and highest enzyme activity after kept for 120 min. Increase of storage temperature to over 50°C inhibits the activity of above 58% after 10 min treatment, while no activity is detected after incubation for 60 min at 60 or 70°C
t1/2: 10 min for wild-type enzyme and mutant enzyme E277K, 4 min for mutant enzymes E277Q and N279H, 25 min for mutant enzyme I278F, less than 2 min for mutant enzymes E277G, E277A, E277D, E277H, E277N, E277V, D275E and D276E
recombinant tagged wild-type enzyme and mutant enzyme show about 90% remaining activity after 10 min, the nontagged wild-type enzyme shows about 40% remaining activity after 10 min