1.14.11.53: mRNA N6-methyladenine demethylase
This is an abbreviated version!
For detailed information about mRNA N6-methyladenine demethylase, go to the full flat file.
Word Map on EC 1.14.11.53
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1.14.11.53
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demethylation
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demethylases
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mettl14
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reader
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writer
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erasers
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ythdf1
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methyltransferases
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epitranscriptomic
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m6a-related
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fto-mediated
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m6a-dependent
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hnrnpa2b1
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methyltransferase-like
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hnrnpc
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lasso
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m6a-modified
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igf2bp1
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merip
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merip-seq
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m6a-binding
- 1.14.11.53
-
demethylation
- demethylases
- mettl14
-
reader
-
writer
-
erasers
-
ythdf1
- methyltransferases
-
epitranscriptomic
-
m6a-related
-
fto-mediated
-
m6a-dependent
-
hnrnpa2b1
-
methyltransferase-like
-
hnrnpc
-
lasso
-
m6a-modified
-
igf2bp1
-
merip
-
merip-seq
-
m6a-binding
Reaction
Synonyms
AlkB homolog 5, ALKBH10B, ALKBH5, ALKBH5 demethylase, ALKBH9B, alkylation repair homolog protein 5, fat mass and obesity-associated enzyme, fat mass and obesity-associated protein, FTO, m6A mRNA demethylase, m6A RNA demethylase, m6A-RNA demethylase, N6-methyladenosine demethylase, RNA N6-methyladenine demethylase
ECTree
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Cloned
Cloned on EC 1.14.11.53 - mRNA N6-methyladenine demethylase
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a plasmid with the vector backbone pNIC28-Bsa4 encoding a hexahistidine-tagged ALKBH566292 construct is transformed into Escherichia coli BL21 (DE3) cells
expression in Escherichia coli BL21 Star (DE3)
mutants of human Alkbh5 are amplified by PCR and subcloned into a modified pET-28a (Novagen) vector encoding a tobacco etch virus protease recognition site. The final clones are verified by DNA sequencing. All of the recombinant plasmids are transformed into Escherichia coli strain BL21(DE3)
the human ALKBH5 catalytic AlkB domain (residues 74294) is subcloned into pET28a-MHL vector and expressed. The cloned vector is transformed into Escherichia coli BL21-V2R-pRARE2. Recombinant protein is produced at 16°C as an N-terminal His-tagged protein
Wild-type and various mutant enzymes cloned into pET-15b are expressed in Escherichia coli strain BL21