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2.1.1.179: 16S rRNA (guanine1405-N7)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (guanine1405-N7)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.179

Reaction

S-adenosyl-L-methionine
+
guanine1405 in 16S rRNA
=
S-adenosyl-L-homocysteine
 +
N7-methylguanine1405 in 16S rRNA

Synonyms

16S rRNA (m7G1405) methyltransferase, 16S rRNA methyltransferase, 16S rRNA N7 G1405 methyltransferase, 16S-RMTase, ArmA, FmrO, G1405 methyltransferase ArmA, GrmA, GrmB, Kmr, Krm, M7G1405 MTase, methyltransferase RmtC, methyltransferase Sgm, N7-G1405 16S-RMTase, NbrB, RmtA, RmtB, RmtC, RmtD, RmtD2, RmtF, RmtG, sgm, Sgm methyltransferase, Sgm MTase, sisomicin-gentamicin methylase, sisomicin-gentamicin methyltransferase, sisomicin-gentamicin resistance methylase, Smr1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.179 16S rRNA (guanine1405-N7)-methyltransferase

Engineering

Engineering on EC 2.1.1.179 - 16S rRNA (guanine1405-N7)-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E182A
-
tobramycin MIC is identical with that of wild-type RmtB
H50A
-
tobramycin MIC is drastically reduced compared to wild-type enzyme
H81A
-
tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme
K14A
-
tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme
K174A
-
tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme
R17A
-
tobramycin MIC is identical with that of wild-type RmtB
R181A
-
tobramycin MIC is drastically reduced compared to wild-type enzyme
R48A
-
tobramycin MIC is identical with that of wild-type RmtB
S83A
-
tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme
Y56F
-
tobramycin MIC is identical with that of wild-type RmtB
D156A
D158A
mutant with drastically increased sensitivity to kanamycin
D182A
E205A
E267A
G135A
K199A
K54A
mutant with drastically increased sensitivity to kanamycin
R108A
mutant with drastically increased sensitivity to kanamycin
R187S
mutant with drastically increased sensitivity to kanamycin
R187S/G212S
mutant with strongly reduced activity
R236A
R433A
mutant with drastically increased sensitivity to kanamycin
H54A
inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin
H54E
inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin
K20E
mutation eliminates 30S binding affinity, mutant strain is sensitive to kanamycin and gentamicin
K72E
mutation reduces 30S binding affinity about 5fold, resistance to kanamycin and gentamcin is reduced
R50E
mutation reduces 30S binding affinity about 11-13fold. Mutant strain is sensitive to kanamycin and gentamicin
R68E
mutation reduces 30S binding affinity about 11-13fold, resistance to kanamycin and gentamcin is reduced
additional information