2.1.1.57: methyltransferase cap1 This is an abbreviated version! For detailed information about methyltransferase cap1, go to the full flat file .
Reaction
S-adenosyl-L-methionine +
a 5'-(N7-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] =
S-adenosyl-L-homocysteine +
a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Synonyms (nucleoside-2'-O-)-methyltransferase, (nucleoside-2'O)-methyltransferase, 2'-O methyltransferase, 2'-O MTase, 2'-O-methyltransferase, 2'-O-MTase, 2'O methyltransferase, 2'O-MTase, 2'OMTases, cap-specific 2'-O-methyltransferase, cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, cap1 2-O'-ribose methyltransferase, CMTr1, EC 2.1.1.58, FtsJ methyltransferase domain-containing 2, FTSJD2, ISG95, KIAA0082, L protein domain VI, large polymerase protein domain VI, messenger ribonucleate nucleoside 2'-methyltransferase, messenger RNA (nucleoside-2'-)-methyltransferase, methyltransferase, messenger ribonucleate nucleoside 2'-, mRNA (nucleoside-2'-O)-methyltransferase, mRNA 5' cap-specific (nucleoside-2'-O)-methyltransferase, mRNA cap (nucleoside-2'-O)-methyltransferase, mRNA cap methyltransferase, mRNA cap-specific 2'-O-methyltransferase, MT57, Mtr1, nonstructural protein 16, nonstructural protein 16 methyltransferase, nonstructural protein 5 methyltransferase, NS5, NS5 MTase, NS5MTase, nsp10/16 complex, nsp10/nsp16 complex, nsp16, nsp16 protein, nsp16/nsp10 protein complex, ribose 2'-O methyltransferase, RNA (nucleoside-2'O)-methyltransferase, RNA cap 2'-O-methyltransferase, RNA cap 2'-O-MTase, RNA cap 2'O-MTase, RNA cap1 methyltransferase, RNA capping enzyme domain in protein A, S-adenosyl-L-methionine-dependent mRNA cap MTase, TbMT48, TbMT57, VP39, VP4
ECTree
Inhibitors
Inhibitors on EC 2.1.1.57 - methyltransferase cap1
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3'-deoxy-ribavirin 5'-triphosphate
binds almost as efficiently as ribavirin 5-triphosphate
5',N8-Adenosyl-alpha,beta-diaminobutyric acid
-
moderate
5'-S-isobutylthio-5'-deoxyadenosine
-
about 25% inhibition at 0.1 mM
5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide triphosphate
-
about 40% inhibition at 0.1 mM
7-methylguanosine(5')pppN-dinucleotides
-
weak, competitive
deazaadenosine
-
about 5% inhibition at 0.1 mM
Mg2+
-
almost complete inhibition at 100 mM
ribavirin
-
about 10% inhibition at 0.1 mM
ribavirin 5'-triphosphate
50% inhibition at 0.1 mM
ribavirin triphosphate
-
about 50% inhibition at 0.1 mM
S-(2-azaadenosyl)-L-homocysteine
-
-
S-(3'-aminoadenosyl)-L-homocysteine
-
-
S-(3-deazaadenosyl)-L-homocysteine
-
strong
S-(5'-adenosyl)-L-cysteine
-
about 25% inhibition at 0.1 mM
S-(8-azaadenosyl)-L-homocysteine
-
weak
S-(N6-Dimethyl-3-deazaadenosyl)-L-homocysteine
-
weak
S-(N6-Methyladenosyl)-L-homocysteine
-
-
S-5'-adenosyl-L-cysteine
-
about 5% inhibition at 0.1 mM
S-Adenosyl-D-homocysteine
-
-
S-adenosyl-L-cysteine
-
moderate
S-adenosyl-L-homocysteine
S-Adenosyl-L-homocysteine sulfone
-
strong
S-adenosyl-L-homocysteine sulfoxide
-
strong
S-Aristeromycinyl-L-homocysteine
-
-
S-Cytidyl-L-homocysteine
-
weak
S-Inosyl-L-homocysteine
-
weak
S-Tubercidinyl-L-homocysteine
-
strong
S-Uridyl-L-homocysteine
-
weak
A9145C
-
strong
A9145C
-
i.e. amino acid modified structure analogue of adenosyl-L-homocysteine, competitive
S-adenosyl-L-homocysteine
-
complete inhibition at 0.1 mM
S-adenosyl-L-homocysteine
-
-
S-adenosyl-L-homocysteine
-
about 25% inhibition at 0.1 mM
S-adenosyl-L-homocysteine
-
cap I-methyltransferase; strong
S-adenosyl-L-homocysteine
-
inhibitory at concentrations beyond 0.002 mM
S-adenosyl-L-homocysteine
-
strong
S-adenosyl-L-homocysteine
-
-
S-adenosyl-L-homocysteine
-
product inhibition, competitive
S-adenosyl-L-homocysteine
-
complete inhibition at 1 mM
sinefungin
-
complete inhibition at 0.1 mM
sinefungin
-
about 5% inhibition at 0.1 mM
sinefungin
-
i.e. A9145, competitive
additional information
-
peptides K12 (aa 68-80 of nsp10), and K29 (aa 68-96 of nsp10) decrease the S-adenosyl-L-methionine (SAM) binding by nsp16 in a dose-dependent manner. When 0.4 mM of K29 is used, it almost abolishes the SAM-binding activity of nsp16. In contrast, the peptides K8 (aa 100-107 of nsp10), K10 (aa 91-100 of nsp10) and K20 (aa 40-59 of nsp10), which cannot inhibit the 2'-O-MTase activity of nsp16, have no effect on SAM binding of nsp16, even when the final concentration of 0.5 mM is applied
-
additional information
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EDTA
-
additional information
-
no inhibition by poly(A), poly(U), poly(C) or poly(G)
-