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2.1.1.85: protein-histidine N-methyltransferase

This is an abbreviated version!
For detailed information about protein-histidine N-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
protein L-histidine
=
S-adenosyl-L-homocysteine
+
protein Ntau-methyl-L-histidine

Synonyms

actin His73 nmethyltransferase, actin histidine methyltransferase, actin histidine-N3 methyltransferase, actin-specific histidine methyltransferase, actin-specific histidine N-methyltransferase, actin-specific methyltransferase, methyltransferase, protein (histidine), protein (actin) histidine MTase, protein methylase IV, S-adenosyl methionine:protein-histidine N-methyltransferase, SET domain protein 3, setd3

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.85 protein-histidine N-methyltransferase

Engineering

Engineering on EC 2.1.1.85 - protein-histidine N-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N255A
the mutant has increased lysine methylation activity and decreased histidine methylation activity compared to the wild type enzyme
N255V
the mutant has increased lysine methylation activity and decreased histidine methylation activity compared to the wild type enzyme
N256A
the mutant shows severely reduced activity compared to the wild type enzyme
N256D
the mutant shows severely reduced activity compared to the wild type enzyme
N256Q
the mutant shows about 50% activity compared to the wild type enzyme
N278A
the mutant shows severely reduced activity compared to the wild type enzyme
R215A
the mutant shows severely reduced activity compared to the wild type enzyme
R316A
the mutant shows severely reduced activity compared to the wild type enzyme
R75A
the mutant shows severely reduced activity compared to the wild type enzyme
Y312A
catalytical mutant with residual activity
Y313F
the mutant shows severely reduced activity compared to the wild type enzyme