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2.1.4.1: glycine amidinotransferase

This is an abbreviated version!
For detailed information about glycine amidinotransferase, go to the full flat file.

Word Map on EC 2.1.4.1

Reaction

L-arginine
+
glycine
=
L-ornithine
+
Guanidinoacetate

Synonyms

AGAT, arginine-glycine amidinotransferase, arginine-glycine transamidinase, arginine:glycine amidinotransferase, CyrA, EC 2.6.2.1, GAT, GATM, glycine amidinotransferase, glycine aminotransferase, glycine transamidinase, L-arginine:glycine amidinotransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.4 Amidinotransferases
                2.1.4.1 glycine amidinotransferase

Purification

Purification on EC 2.1.4.1 - glycine amidinotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 fractions of enzyme, designated alpha- and beta transamidase individually purified
-
recombinant N-terminal His6-tagged CyrA from Escherichia coli strain BL21(DE3)
recombinant wild-type and mutant N-terminal His6-tagged CyrAs from Escherichia coli strain BL21(DE3) by metal affinity chromatography to over 95% purity