2.3.1.18: galactoside O-acetyltransferase

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For detailed information about galactoside O-acetyltransferase, go to the full flat file.

Reaction

Synonyms

acetyltransferase, galactoside, galactoside acetyltransferase, galactoside O-acetyltransferase, galactoside transacetylase, GAT, LacA, SACOL2570, thiogalactoside acetyltransferase, thiogalactoside transacetylase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.18 galactoside O-acetyltransferase

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Results	in table
902	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
1	Expression
2	General Information
11	Inhibitors
2	Ki Value [mM]
38	KM Value [mM]
3	Localization
3	Metals/Ions
8	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
27	Organism
12	PDB ID
2	pH Optimum
11	Protein Variants
9	Purification (Commentary)
4	Reaction
2	Reaction Type
16	Reference
5	Specific Activity [micromol/min/mg]
2	Storage Stability
59	Substrates and Products (Substrate)
6	Subunits
13	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
23	Turnover Number [1/s]

Purification

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Purification on EC 2.3.1.18 - galactoside O-acetyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

affinity chromatography

Escherichia coli

-

486463

K12 strain H30000

Escherichia coli

-

486464

recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)RIPL by nickel affinity chromatography, tag cleavage with TEV protease, another step of nickel affinity chromatography, and gel filtration

Staphylococcus aureus

Q5HCZ5

736706

recombinant His6-tagged wild-type and mutant enzymes by nickel affinity chromatography, ion exchange chromatography, and gel filtration

Escherichia coli

-

672425

strain ML308

Escherichia coli

-

486460, 486461, 486464

using a HiTrap metal-chelating affinity chromatography column, a HiTrap desalting, and a Superdex 200 column

Escherichia coli

-

672425

wild-type and mutant enzyme

Escherichia coli

-

486466