2.3.1.221: noranthrone synthase
This is an abbreviated version!
For detailed information about noranthrone synthase, go to the full flat file.
Word Map on EC 2.3.1.221
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2.3.1.221
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sporopollenin
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tetraketide
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exine
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anther
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microspore
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tapetal
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reductase1
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halogenase
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prenyltransferase
- 2.3.1.221
-
sporopollenin
-
tetraketide
-
exine
-
anther
-
microspore
-
tapetal
-
reductase1
- halogenase
- prenyltransferase
Reaction
7 malonyl-CoA + = 7 CoA + + + 7 CO2 + 2 H2O
Synonyms
norsolorinic acid anthrone synthase, norsolorinic acid anthrone-producing polyketide synthase, PksA, polyketide synthase A
ECTree
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Cloned
Cloned on EC 2.3.1.221 - noranthrone synthase
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expression of N-terminally His6-tagged enzyme domains in Escherichia coli strain BL21(DE3)
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expression of recombinant His-tagged thioesterase/Claisen cyclase in Escherichia coli, expression of the selenomethionine-labeled PksA TE in Escherichia coli methionine auxotroph strain B834(DE3)
expression of wild-type and mutant proteins in Escherichia coli strains BL21(DE3) or Rosetta2(DE3)
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gene pksA, cloning of various mono-, di-, and tridomains for combinatorial reconstitution experiments, expression of the either C- or N-terminally His6-tagged protein fragments in Escherichia coli
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gene pksA, DNA and amino acid sequence determination and analysis