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2.3.1.221: noranthrone synthase

This is an abbreviated version!
For detailed information about noranthrone synthase, go to the full flat file.

Word Map on EC 2.3.1.221

Reaction

7 malonyl-CoA +

hexanoyl-[acyl-carrier protein]
= 7 CoA +
norsolorinic acid anthrone
+
[acyl-carrier protein]
+ 7 CO2 + 2 H2O

Synonyms

norsolorinic acid anthrone synthase, norsolorinic acid anthrone-producing polyketide synthase, PksA, polyketide synthase A

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.221 noranthrone synthase

Cloned

Cloned on EC 2.3.1.221 - noranthrone synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of N-terminally His6-tagged enzyme domains in Escherichia coli strain BL21(DE3)
-
expression of recombinant His-tagged thioesterase/Claisen cyclase in Escherichia coli, expression of the selenomethionine-labeled PksA TE in Escherichia coli methionine auxotroph strain B834(DE3)
expression of wild-type and mutant proteins in Escherichia coli strains BL21(DE3) or Rosetta2(DE3)
-
gene pksA, cloning of various mono-, di-, and tridomains for combinatorial reconstitution experiments, expression of the either C- or N-terminally His6-tagged protein fragments in Escherichia coli
-
gene pksA, DNA and amino acid sequence determination and analysis