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2.3.2.15: glutathione gamma-glutamylcysteinyltransferase

This is an abbreviated version!
For detailed information about glutathione gamma-glutamylcysteinyltransferase, go to the full flat file.

Word Map on EC 2.3.2.15

Reaction

glutathione
+
[Glu(-Cys)]n-Gly
=
Gly
 +
[Glu(-Cys)]n+1-Gly

Synonyms

AdPCS1, AdPCS2, AdPCS3, alr0975, AtPCS, AtPCS1, AtPCS2, AtPCSI, BjPCS1, CaPCS1, CaPCS2, CePCS, DaPCS1, gamma-EC dipeptidyl(trans)peptidase, gamma-glutamyl-cysteine transpeptidase, gamma-glutamylcysteine dipeptidyl transpeptidase, gamma-glutamylcysteine transpeptidase, gamma-glutamylcysteinyl dipeptidyl transpeptidase, gamma-glutamylcysteinyl transpeptidase, glutathione gamma-glutamylcysteinyltransferase, LjPCS1, LjPCS3, MnPCS1, MnPCS2, NsPCS, OsPCS1, OsPCS15, OsPCS1a, OsPCS1b, OsPCS1c, OsPCS1full, OsPCS2, OsPCS5, PC synthase, PCS, PCS1, PCS15, PCS2, PCS5, phytochelatin synthase, phytochelatin synthase 1, phytochelatin synthase 2, phytochelatin synthase1, pPhytochelatin synthase, pseudo-phytochelatin synthase, SrPCS, SrPCS1, SrPCS2, SrPCS3, SrPCS4, TtpsiPCS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.15 glutathione gamma-glutamylcysteinyltransferase

Expression

Expression on EC 2.3.2.15 - glutathione gamma-glutamylcysteinyltransferase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after 24 h of exposure to 0.02 mM CdSO4, CuSO4 or ZnSO4, isoform PCS1 expression increases significantly. The increases in roots are in order of 4.34fold, 0.26fold or 2.82fold after exposure to CdSO4, CuSO4 or ZnSO4, respectively. Reduced L-glutathione stimulates isoform PCS1 expression in roots, stems and leaves (1.45-2.85fold)
enzyme expression increases gradually in the leaves when given a treatment of 0.1, 0.2 or 0.5 mM CdCl2 for 4 or 8 h
-
enzyme expression is induced after cadmium and copper treatments
-
expression of AtPCS2 is significantly decreased in response to high concentrations of metals Ni2+ (0.1 mM), Mg2+ (1.0 mM), Cd2+ (0.3 mM), Hg2+ (0.030 mM), Zn2+ (0.5 mM), and Pb2+ (0.5 mM), as well as by abscisic acid (0.1 mM), PEG (15%) drougth, and cold (4°C) stress, overview
expression of AtPCS2 is significantly increased in response to 100 and 200 mM NaCl treatment. In addition, transgenic Arabidopsis subjected to salt stress exhibit enhanced proline accumulation and reduced Na+/K+ ratios compared to wild-type plants. The enzyme expression is also induced by H2O2 at 20 mM
expression of gene AdPCS 1 is responsive to stress through heavy metals
expression of OsPCS1 is upregulated by As(III) stress in the roots
expression of PCS1 is induced in root, stem, and leaf tissues within 24 h of exposure to 0.3 mM Zn2+ and 0.03-0.1 mM Cd2+
expression of PCS15 is induced by Cd2+ and As5+ ions
-
expression of PCS2 is induced in root, stem, and leaf tissues within 24 h of exposure to 0.3 mM Zn2+ and 0.03-0.1 mM Cd2+
expression of PCS5 is induced by Cd2+ and As5+ ions
-
expression patterns of AtPCS2 in Arabidopsis thaliana in response to various heavy metals or abiotic stresses, overview
in the developing rice seeds, the Cd exposure significantly upregulates the transcript levels of OsPCS1 (2.5fold). Analysis of the qRT-PCR revealed that the transcript levels of both OsPCS1 and OsPCS2a decreased drastically even after exposure to Cd stress
L-buthionine-sulfoximine can inhibit isoform PCS1 expression in roots, stems and leaves
the expression of OsPCS2 is not significantly affected by As(III) stress in the roots
the expression of the enzyme in leaves of Nelumbo nucifera is dramatically increased in response to cadmium (0.4 mM) treatment. In the root, enzyme expression initially raises slightly after exposure to Cd, but falls back after 1 h. When exposed to cadmium (0.1 mM) stress, Arabidopsis transgenic plants heterologous expressing the enzyme accumulate more cadmium when compared with wild type
-
the expression pattern of OsPCS1 splice variants is not changed by Cd or As stress in both shoots and roots
the PCS gene in Chlamydomonas acidophila shows very strong induction by cadmium
the PCS gene in Dunaliella acidophila is not induced by cadmium, most likely due to gene duplication
the two transcript isoforms (OsPCS2a and OsPCS2b) of OsPCS2 gene demonstrate contrasting expression pattern upon Cd stress. The OsPCS2a mRNA level remains almost unaltered in case of the root tissues, but profoundly increased (2.5-15.6folds) between 3 and 72 h in shoot tissues. On the other hand, the amount of OsPCS2b transcript is significantly enhanced with maximum expression maintained between 12 and 72 h in roots, but is significantly downregulated in shoots until 72 h, when its expression rapidly increased to about 4.5fold upon Cd treatment. In the developing rice seeds, the Cd exposure significantly upregulates the transcript levels of OsPCS2a (about 6fold), whereas the mRNA level of OsPCS2b is reduced by about 25%. Analysis of the qRT-PCR revealed that the transcript levels of both OsPCS1 and OsPCS2a decreased drastically even after exposure to Cd stress
there is no significant change in transcript accumulation of PCS1 following Cd2+ treatment
up-regulated under cadmium stress conditions