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2.4.1.135: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase

This is an abbreviated version!
For detailed information about galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.135

Reaction

UDP-alpha-D-glucuronate
+
[protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine
=
UDP
 +
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine

Synonyms

beta-1,3-glucuronyltransferase 1, beta1,3-glucuronosyltransferase I, beta1,3-glucuronosyltransferase-I, beta1,3-glucuronyltransferase I, galactose beta1,3 glucuronosyl transferase-I, galactose beta1,3-glucuronosyltransferase-I, galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase, Galbeta1,3-glucuronosyltransferase, GlcAT-1, GlcAT-I, GlcAT-P, GlcATI, GlcUAT-I, glucuronosyltransferase I, glucuronyltransferase-I, glycosaminoglycan glucuronyltransferase I, glycosyltransferases GlcAT1, UDP-GlcUA:Gal Beta-1,3-Gal-R glucuronyltransferase, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, UDP-glucuronic acid:Galbeta1,3Gal-R glucuronsyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.135 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase

Cloned

Cloned on EC 2.4.1.135 - galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chimeric proteins composed of mutant GlcATI fused to IgG binding domain of protein A or to green fluoresecent protein do not yield the protein at the expected mass
-
expression in COS-1 cell, a soluble form of GlcAT-I generated by replacing the first 43 amino acids of GlcAT-I with a cleavable insulin signal sequence and a protein A IgG-binding domain
-
expression in Cos-1 cells
expression in Pichia pastoris
expression of full-length GlcAT-I in COS-1 cells
-
gene B3GAT3, a unigene, DNA and amino acid sequence determination and analysis, sequence comparison with the human enzyme and phylogenetic analysis
-
genetic interaction between dGlcAT-P and dC1GalT1, recombinant expression of dGlcAT-I, dGlcAT-P-II, and dGlcAT-S-I in Spodoptera frugiperda Sf21 insect cells
GlcAT-I cDNA probe are subcloned into Bluescript plasmid vectors and sequenced. Localization of GlcAT-1 and the pseudogene to two distinct chromosomes, 11q12-q13
transfection of a CHO cell mutant defective in GlcUAT-I with the hamster cDAN restores glycosaminoglycan assembly in vivo