2.4.1.173: sterol 3beta-glucosyltransferase

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For detailed information about sterol 3beta-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

Atg26, AtSGT, autophagy-related protein 26, CG synthetase, pUGT51, ScAtg26, SGT, SGT1, SGT2, SGT3, SGT4, SGTL1, SgtL2, SgtL4, SlSGT1, SlSGT2, SlSGT3, SlSGT4, Solyc04g051150.2.1, Solyc04g071540.2.1, Solyc06g007980.2.1, Solyc09g061860.2.1, sterol glucosyltransferase, sterol glucosyltransferase Ugt51/Paz4, sterol glycosyltransferase, sterol-beta-D-glucosyltransferase, sterol:UDPG glucosyltransferase, UDP-Glc:sterol glucosyltransferase 80B1, UDP-glucose sterol glucosyltransferase, UDP-glucose sterol glycosyltransferase, UDP-glucose-sterol beta-glucosyltransferase, UDP-glucose-sterol glucosyltransferase, UDP-glucose:sterol glucosyltransferase, UDP-glycosyltransferase 51, UDPG-SGTase, UDPG:sterol glucosyltransferase, UDPglucose:sterol glucosyltransferase, Ugt51, Ugt51/Paz4, ugt51E1, UGT713B1, UGT80A2, UGT80B1, uridine diphosphoglucose-poriferasterol glucosyltransferase, uridine diphosphoglucose-sterol glucosyltransferase, USGT80B1

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.173 sterol 3beta-glucosyltransferase

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Results	in table
48	Activating Compound
1416	AA Sequence
4	Application
2	CAS Registry Number
31	Cloned(Commentary)
1	Crystallization (Commentary)
5	Disease/ Diagnostics
5	Expression
47	General Information
8	IC50 Value
71	Inhibitors
3	kcat/KM [mM/s]
2	Ki Value [mM]
51	KM Value [mM]
53	Localization
32	Metals/Ions
15	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
99	Organism
2	Pathway
4	PDB ID
16	pH Optimum
2	pH Range
23	pH Stability
1	pI Value
22	Protein Variants
25	Purification (Commentary)
1	Reaction
1	Reaction Type
73	Reference
68	Source Tissue
3	Specific Activity [micromol/min/mg]
3	Storage Stability
268	Substrates and Products (Substrate)
10	Subunits
110	Synonyms
1	Systematic Name
31	Temperature Optimum [°C]
1	Temperature Range [°C]
11	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.4.1.173 - sterol 3beta-glucosyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant UGT51 alone and in complex with UDP-glucose, hanging drop vapor diffusion method, mixing of 20 mg/ml protein in 20 mM Tris-HCl, 500 mM NaCl, and 2 mM DTT, with reservoir solution containing 0.2 M ammonium acetate, 0.1 M HEPES, pH 7.5, 25% w/v PEG 3350 for the free enzyme, or containing 0.2 M MgCl2, 0.1 M Tris, pH 8.5, and 25% w/v PEG 3350 for the enzyme complex, receiving microcrystals, optimized crystallization protocol involves dispensing 0.0015 ml of 20 mg/ml protein and 0.001 ml of reservoir solution (containing 0.2 M MgCl2, 0.1 M Tris pH 8.5 and 25% w/v PEG 3350) and 500 nl of 0.001 diluted crystal seed stock solution, rod protein crystals appear after 3 days, 14°C, crystals are soaked in cryoprotectant solution with 5 mM UDP-glucose, X-ray diffraction structure determination and analysis at 2.77 A and 1.9 A resolutions, respectively, molecular replacement using the structure of GtfD (PDB ID 1rrv) as the search model, modeling

Saccharomyces cerevisiae

Q06321

759680