2.4.1.173: sterol 3beta-glucosyltransferase This is an abbreviated version!go to the full flat file .
Word Map on EC 2.4.1.173 Reaction
UDP-glucose a sterol UDP a sterol 3-beta-D-glucoside
Synonyms Atg26, AtSGT, autophagy-related protein 26, CG synthetase, pUGT51, ScAtg26, SGT, SGT1, SGT2, SGT3, SGT4, SGTL1, SgtL2, SgtL4, SlSGT1, SlSGT2, SlSGT3, SlSGT4, Solyc04g051150.2.1, Solyc04g071540.2.1, Solyc06g007980.2.1, Solyc09g061860.2.1, sterol glucosyltransferase, sterol glucosyltransferase Ugt51/Paz4, sterol glycosyltransferase, sterol-beta-D-glucosyltransferase, sterol:UDPG glucosyltransferase, UDP-Glc:sterol glucosyltransferase 80B1, UDP-glucose sterol glucosyltransferase, UDP-glucose sterol glycosyltransferase, UDP-glucose-sterol beta-glucosyltransferase, UDP-glucose-sterol glucosyltransferase, UDP-glucose:sterol glucosyltransferase, UDP-glycosyltransferase 51, UDPG-SGTase, UDPG:sterol glucosyltransferase, UDPglucose:sterol glucosyltransferase, Ugt51, Ugt51/Paz4, ugt51E1, UGT713B1, UGT80A2, UGT80B1, uridine diphosphoglucose-poriferasterol glucosyltransferase, uridine diphosphoglucose-sterol glucosyltransferase, USGT80B1
ECTree
Activating Compound
Activating Compound on EC 2.4.1.173 - sterol 3beta-glucosyltransferase
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dithiothreitol
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25% stimulation at 1 mM
EGTA
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50% of inhibition at 13 mM
Emulphogen BC-720
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stimulation al low concentrations
ethanol
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increase of activity at 7-20%
KOH
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treatment with KOH pH 10.4 increase activity about 65%, hydroxyl ions can be used in place of detergents to reveal latent enzymatic sites
p-chloromercuribenzoate
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potent inhibitory effect indicating that free SH groups are essential for activity
phosphatidylethanolamine
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increases 125% of activity
phosphatidylglycerol
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increases Vmax
phosphatidylserine
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33% increase of activity
Triton
activates isozyme GhSGT2
Triton N-101
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stimulation al low concentrations
-
Triton X-114
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stimulation al low concentrations
Tween-20
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2fold activity stimulation at 0.1%
Tween-40
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70% activity stimulation at 0.05%
Tween-60
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35% activity stimulation at 0.1%
additional information
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stimulated by acylglycerols containing unsaturated fatty acid residues
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2-mercaptoethanol
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slightly stimulation
2-mercaptoethanol
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activated by
2-mercaptoethanol
activates the isozyme
2-mercaptoethanol
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10% stimulation at 1 mM
ATP
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15% stimulation at 0.01 mM
ATP
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slightly stimulation at low concentration
EDTA
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activated by
EDTA
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15% stimulation at 0.1 mM
EDTA
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70% of inhibition at 13 mM
lecithin
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stimulation by cotton and soybean lecithin but not with egg lecithin, appears to increase Vmax with sitosterol and Km of UDP-glucose
lecithin
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20-25% stimulation
phosphatidylcholine
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33% increase of activity
phosphatidylcholine
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increases Vmax
phospholipase
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treatment with phospholipases A, C and D lowers activity 21-29%, the effect of phospholipase A is completely reverse by phosphatidylethanolamine but not by phosphatidylcholine or phosphatidylserine, after phospholipase C or D treatment each phospholipid brings about a partial recovery of activity but phosphatidylethanolamine is superior
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phospholipase
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phospholipase A2, slight inhibition of latent activity but stimulates the patent activity, it can be used in place of detergents to reveal latent enzymatic sites
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Phospholipids
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not stimulatory
Phospholipids
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stimulated by negatively charged phospholipids
Phospholipids
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phosphatidylcholine + phosphatidylglycerol or phosphatidylcholine + phosphatidic acid increase Vmax and Km with respect to phosphatidylcholine alone
Phospholipids
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phosphatidylcholine or phosphatidylglycerol increase Vmax
Phospholipids
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the highest Vmax and Km is observed for the ratios 3:1 of phosphatidylcholine + phosphatidylglycerol and 3:1 phosphatidylcholine + phosphatidic acid
Phospholipids
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stimulation of activity by solubilization of enzyme still membrane-bound
Triton X-100
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activity stimulated at 0.1-0.5%
Triton X-100
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activity stimulated by 0.1-0.5% with highest stimulation at 0.25%
Triton X-100
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activity stimulated at 0.3%
Triton X-100
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activity stimulated by 0.1-1% with 6.5fold stimulation at 0.1%, highest activity stimulation with respect to others non-ionic detergents
Triton X-100
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130fold at 0.1%
Triton X-100
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up to 0.03% Triton X-100 in the assay mixture, the catalytic activity is enhanced
Triton X-100
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stimulation at low concentrations, at intermediate concentration 0.4-0.8% this stimulation is abolished, it appears to solubilize enzyme still membrane-bound
Triton X-100
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stimulation of plasma membrane vesicles at 0.1-0.2% but not of endoplasmic reticulum vesicles
2.4.1.173 sterol 3beta-glucosyltransferase
results (
results (
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