2.4.1.231: alpha,alpha-trehalose phosphorylase (configuration-retaining)

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For detailed information about alpha,alpha-trehalose phosphorylase (configuration-retaining), go to the full flat file.

Reaction

Synonyms

PoTPase, ScTPase, TP2, TP3, trehalose phosphorylase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.231 alpha,alpha-trehalose phosphorylase (configuration-retaining)

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Results	in table
4	Activating Compound
9	AA Sequence
1	Application
1	CAS Registry Number
2	Cloned(Commentary)
4	General Information
2	General Stability
40	Inhibitors
2	kcat/KM [mM/s]
21	Ki Value [mM]
44	KM Value [mM]
1	Metals/Ions
12	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
27	Organism
1	Pathway
15	pH Optimum
3	pH Range
2	pH Stability
2	pI Value
10	Protein Variants
10	Purification (Commentary)
7	Reaction
2	Reaction Type
25	Reference
24	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
57	Substrates and Products (Substrate)
7	Subunits
14	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
9	Temperature Stability [°C]
32	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 2.4.1.231 - alpha,alpha-trehalose phosphorylase (configuration-retaining)

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

20

Schizophyllum commune

-

unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize

644898

30

2 entries

35

Grifola frondosa

-

stable up to 35°C, decrease of activity above

644906

4

Schizophyllum commune

-

unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize

644898

40

2 entries

45 - 50

Lactobacillus acidophilus

-

-

720962

additional information

Pleurotus ostreatus

-

PoTPase has a half life of approximately 1 h at 30°C

674984

30

Lentinus sajor-caju

Q9UV63

stable below

644901

30

Pleurotus ostreatus

-

half-life: approximately 1 h in absence of stabilizing compound, 11.5 h in presence of 300 mM alpha,alpha-trehalose, 6.5 h in presence of 20% w/v glycerol, 70 h in presence of 26% w/v polyethylenglycol. Covalent modification of PoTPase with activated derivatives of PEG 5000 increases the stability by up to 600fold

674984

40

Agaricus bisporus

-

almost complete loss of activity after 60 min

644907

40

Lentinus sajor-caju

Q9UV63

decrease of activity

644901