2.4.1.302: L-demethylnoviosyl transferase

This is an abbreviated version!
For detailed information about L-demethylnoviosyl transferase, go to the full flat file.

Reaction

Synonyms

NovM

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.302 L-demethylnoviosyl transferase

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Results	in table
65	AA Sequence
1	Cloned(Commentary)
1	General Information
2	Inhibitors
7	kcat/KM [mM/s]
1	Ki Value [mM]
5	KM Value [mM]
1	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
3	Organism
5	Pathway
1	pH Optimum
1	Purification (Commentary)
1	Reaction
3	Reference
13	Substrates and Products (Substrate)
1	Subunits
3	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
5	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.4.1.302 - L-demethylnoviosyl transferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

dTDP-6-deoxy-L-glucose + novobiocic acid

dTDP + ?

Streptomyces niveus

-

-

723329

-

-

?

dTDP-beta-L-noviose + N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide

dTDP + N-[7-[(6-deoxy-5-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-chromen-3-yl]-4-hydroxy-3-methylbenzamide

Streptomyces sp.

-

-

723619

-

-

?

dTDP-beta-L-noviose + novobiocic acid

dTDP + demethyldecarbamoyl novobiocin

5 entries

dTDP-L-4-dehydro-6-deoxy-glucose + novobiocic acid

dTDP + ?

Streptomyces niveus

-

-

723329

-

-

?

dTDP-L-noviose + 4-nitrophenol

dTDP + ?

Streptomyces niveus

-

kcat/Km is 208000fold lower compared to the value for the natural substrate novobiocic acid

721589

-

-

?

dTDP-L-noviose + 7-hydroxy-4-methylcoumarin

dTDP + ?

Streptomyces niveus

-

kcat/Km is 142000fold lower compared to the value for the natural substrate novobiocic acid

721589

-

-

?

dTDP-L-noviose + 7-hydroxycoumarin

dTDP + ?

Streptomyces niveus

-

kcat/Km is 172000fold lower compared to the value for the natural substrate novobiocic acid

721589

-

-

?

dTDP-L-noviose + cyclonovobiocic acid

dTDP + ?

Streptomyces niveus

-

kcat/KM is 30% compared to the value for the natural substrate novobiocic acid

721589

-

-

?

dTDP-xylose + novobiocic acid

dTDP + ?

Streptomyces niveus

-

-

723329

-

-

?

dTDP-beta-L-noviose + novobiocic acid

dTDP + demethyldecarbamoyl novobiocin

Streptomyces niveus

-

-

723329

i.e. desmethyldescarbamoyl novobiocin

-

?

dTDP-beta-L-noviose + novobiocic acid

dTDP + demethyldecarbamoyl novobiocin

Streptomyces niveus

-

the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin

721589

i.e. demethyldecarbamoyl novobiocin

-

?

dTDP-beta-L-noviose + novobiocic acid

dTDP + demethyldecarbamoyl novobiocin

Streptomyces niveus

-

no activity with TDP-L-rhamnose

721589

-

-

?

dTDP-beta-L-noviose + novobiocic acid

dTDP + demethyldecarbamoyl novobiocin

Streptomyces sp.

-

-

723619

i.e. demethyldecarbamoyl novobiocin

-

?

dTDP-beta-L-noviose + novobiocic acid

dTDP + demethyldecarbamoyl novobiocin

Streptomyces sp.

-

sequential bi-bi catalytic mechanism. NovM binds to both donor and acceptor substrate (novobiocic acid and dTDP-L-noviose) to form a ternary complex, during which process the noviose sugar is transferred from dTDP-L-noviose to novobiocic acid. The glycosylated product, demethyldecarbamoyl novobiocin, is subsequently released from the complex

723619

i.e. demethyldecarbamoyl novobiocin

-

?