Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.2.21: nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase

This is an abbreviated version!
For detailed information about nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.21

Reaction

beta-nicotinate D-ribonucleotide
+
5,6-Dimethylbenzimidazole
=
nicotinate
 +
alpha-ribazole 5'-phosphate
 +
H+

Synonyms

5,6-dimethylbenzimidazole phosphoribosyltransferase, ArsAB, CblT, CobT, CobT2, N(1)-alpha-phosphoribosyltransferase, N1-alpha-phosphoribosyltransferase, NaMN:DMB phosphoribosyltransferase, nicotinate mononucleotide-dimethylbenzimidazole phosphoribosyltransferase, nicotinate mononucleotide:5,6-dimethylbenzimidazolyl phosphoribosyltransferase, nicotinate ribonucleotide:benzimidazole (adenine) phosphoribosyltransferase, NN:DBI PRT, phosphoribosyltransferase, nicotinate mononucleotide-dimethylbenzimidazole, SoArsAB

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.21 nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase

Engineering

Engineering on EC 2.4.2.21 - nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C160A
loss of catalytic efficiency by 79%. Renders the enzyme 5fold less efficient than wild-type. Displays no discernible growth defect in no-carbon essential (NCE) medium containing dicyanocobinamide and glycerol or ethanolamine relative to that of a strain that synthesizes wild-type
G171D
loss of catalytic efficiency by more than 99%, has less affinity for 5,6-dimethylbenzimidazole. Retains activity to support growth, but only when 5,6-dimethylbenzimidazole is present in the medium. Does not support the growth of strain JE2423 on ethanolamine in medium that contains dicyanocobinamide but lacks 5,6-dimethylbenzimidazole
G171D/M325A
requires cobalamin for growth regardless of the carbon source used
G257A
loss of catalytic efficiency by 64%
G257D
loss of catalytic efficiency by more than 99%, has less affinity for 5,6-dimethylbenzimidazole. Does not respond to 5,6-dimethylbenzimidazole and requires cobalamin to grow. Does not support the growth of strain JE2423 on ethanolamine in medium that contains dicyanocobinamide but lacks 5,6-dimethylbenzimidazole
G257D/P12G
is cobalamin auxotroph on glycerol or ethanolamine. P12G does not alter the effect of the G257D mutation
G320D
loss of catalytic efficiency by more than 99%, is unstable, retains activity to support growth, but only when 5,6-dimethylbenzimidazole is present in the medium. Does not support the growth of strain JE2423 on ethanolamine in medium that contains dicyanocobinamide but lacks 5,6-dimethylbenzimidazole
H289A
counteracts the effect of the G320D substitution
M325A
grows in the absence of exogenous 5,6-dimethylbenzimidazole, albeit at a rate slower than that of a strain making wild-type. Addition of 5,6-dimethylbenzimidazole to the medium allows the strain making M325A to grow as fast as a strain making wild-type
P12G
does not require exogenous 5,6-dimethylbenzimidazole for growth on glycerol or ethanolamine
I321S
specific activity with p-cresol and 5,6-dimethylbenzimidazole decreased
M87Q
effect of the M87Q mutation is most pronounced with respect to phenolic specificity, as the specific activity with p-cresol drops by 2.6fold while a 1.2fold increase in activity with 5,6-dimethylbenzimidazole is observed
M87Q/I321S
specific activity with p-cresol and 5,6-dimethylbenzimidazole highly decreased