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2.6.1.52: phosphoserine transaminase

This is an abbreviated version!
For detailed information about phosphoserine transaminase, go to the full flat file.

Reaction

4-phosphooxy-L-threonine
+
2-oxoglutarate
=
(3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate
 +
L-glutamate

Synonyms

3-O-phospho-L-serine:2-oxoglutarate aminotransferase, 3-phosphoserine aminotransferase, AspAT, AtPSAT, AtPSAT1, BCIR PSAT, bmPSAT, EhPSAT, hydroxypyruvic phosphate-glutamic transaminase, L-phosphoserine aminotransferase, phosphohydroxypyruvate transaminase, phosphohydroxypyruvic-glutamic transaminase, phosphoserine aminotransferase, phosphoserine aminotransferase 1, phosphoserine aminotransferase1, phosphoserine aminotransferase2, phosphoserine aminotransferases, PSAT, PSAT alpha, PSAT beta, PSAT-BALC, PSAT-BCIRA, PSAT-ECOLI, PSAT1, PSAT2, PSerAT, serC, sll1559

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.52 phosphoserine transaminase

Cloned

Cloned on EC 2.6.1.52 - phosphoserine transaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms results from alternative splicing, PSATalpha and PSATbeta, cDNA cloned and expressed in Escherichia coli and in human cell lines, leukaemia Jurkat, colon adenocarcinoma COLO 320DM, heptacellular carcinoma HepG2 and weakly in leukaemia MOLT-3, complementation of Saccharomyces cerevisiae SER-1 deletion mutation
cDNA encoding PSAT, single gene mapped on the lower arm of chromosome 4, overexpressed in Escherichia coli AD494 and BL21
cloned and expressed in Escherichia coli
-
cloned and expressed in Escherichia coli KL282 and BL21
-
cloning of the PSAT gene, expression in Escherichia coli
-
coexpression of enzyme mutant SerCR42W/R77W with Escherichia coli pyruvate decarboxylase and alcohol dehydrogenase in Escherichia coli strain BL21(DE3), recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression in Escherichia coli
gene EhPSAT, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene PSAT1, quantitative reverse transcriptase PCR enzyme expression analysis
gene PSAT1, recombinant expression of His6-tagged in Escherichia coli strain BL21 Gold
gene serC, recombinant overexpression in Escherichia coli strain AB2829 harbouring pKD501 plasmid
gene serC, recombinant overexpression in Escherichia coli strain BL21(DE3)
gene serC, recombinant overexpression of enzyme mutant K2G in Escherichia coli strain XL-1 Blue
overexpressed as His6-tagged protein in Escherichia coli cells
quantitative real-time PCR enzyme expression analysis
sequence comparisons and phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression of SUMO-His6-tagged enzyme in Escherichia coli by using a cold-shock promotor