Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.1.108: dolichol kinase

This is an abbreviated version!
For detailed information about dolichol kinase, go to the full flat file.

Word Map on EC 2.7.1.108

Reaction

CTP
+
dolichol
=
CDP
 +
dolichyl phosphate

Synonyms

At3g45040, AtDOK1, cytidine-5-triphosphate (CTP) dependent dolichol kinase, DK, DK1, DOK1, dolichol kinase, dolichol kinase 1, dolichol phosphokinase, dolichol-specific kinase, DOLK, SEC59, Sec59p

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.108 dolichol kinase

Engineering

Engineering on EC 2.7.1.108 - dolichol kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G471D
site-directed mutagenesis,inactive mutant
Q483K
naturally occuring homozygous c.1447C>A DOLK mutation involved in enzyme deficiency and a phenotype with anatomic malformations and multi-systemic dysfunction
G405S
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
G405S/I419S
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells
I419S
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
G405S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S/I419S
-
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells
-
I419S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S/I419S
-
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells
-
I419S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S/I419S
-
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells
-
I419S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S/I419S
-
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells
-
I419S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S/I419S
-
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells
-
I419S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
G405S/I419S
-
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells
-
I419S
-
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype
-
W332G
additional information