2.7.1.159: inositol-1,3,4-trisphosphate 5/6-kinase

This is an abbreviated version!
For detailed information about inositol-1,3,4-trisphosphate 5/6-kinase, go to the full flat file.

Word Map on EC 2.7.1.159

2.7.1.159

phytic

polyphosphate

hexakisphosphate

dehydration

itpks

2-kinase

phosphorus

pentakisphosphate

signalosome

lipid-independent

tnfalpha-induced

1,3,4,5,6-pentakisphosphate

monophosphatase

photomorphogenesis

1,4,5-tris-phosphate

1,3,4,6-tetrakisphosphate

phytate

imp

ikappabalpha

atp-grasp

agriculture

Reaction

1D-myo-inositol 1,3,4,5-tetrakisphosphate

Synonyms

1,3,4-trisphosphate 5/6-kinase, 5/6 kinase, 5/6-kinase, At2G43980, AtITPK1, AtITPK2, AtITPK3, AtITPK4, GmItpk2, inositol 1,3,4 tris phosphate 5/6 kinase-2, inositol 1,3,4 trisphosphate kinase-2, inositol 1,3,4-triphosphate 5/6 kinase, inositol 1,3,4-triphosphate 5/6-kinase, inositol 1,3,4-trisphosphate 5/6 kinase-2, inositol 1,3,4-trisphosphate 5/6-kinase, inositol 1,3,4-trisphosphate 5/6-kinase 1, inositol 1,3,4-trisphosphate 5/6-kinase 4, inositol 1,3,4-trisphosphate 5/6-kinase6, inositol 1,4,5 trisphosphate 3/6 kinase, inositol-1,3,4-trisphosphate kinase, Ins(1,3,4)P3 5/6-kinase, IP56K, Ipk2, ITPK, Itpk-1, ITPK1, ITPK2, ITPK3, More, Os09g0518700, OsITL1, OsITPK6

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.159 inositol-1,3,4-trisphosphate 5/6-kinase

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Results	in table
4	Activating Compound
1415	AA Sequence
1	Application
1	CAS Registry Number
20	Cloned(Commentary)
21	Cofactor
2	Crystallization (Commentary)
5	Disease/ Diagnostics
7	Expression
21	General Information
1	General Stability
7	Inhibitors
3	Ki Value [mM]
4	KM Value [mM]
7	Localization
20	Metals/Ions
7	Molecular Weight [Da]
51	Natural Substrates/ Products (Substrates)
32	Organism
7	Pathway
11	PDB ID
9	pH Optimum
3	pI Value
2	Posttranslational Modification
10	Protein Variants
14	Purification (Commentary)
6	Reaction
5	Reaction Type
29	Reference
46	Source Tissue
8	Specific Activity [micromol/min/mg]
94	Substrates and Products (Substrate)
8	Subunits
53	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
2	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.1.159 - inositol-1,3,4-trisphosphate 5/6-kinase

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purified recombinant enzyme free or in complex with ATP analogue AMP-PCP and substrate inositol-1,3,4-trisphosphate in presence of Mg2+, 30 mg/ml protein in 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 10 mM DTT, mixed with a solution containing 20% PEG 3000, 0.1 M Tris-HCl, pH 7.5, and 0.1 M Ca(OAc)2, several months, crystals are seeded into sitting drops of 15 mg/ml protein, 25% PEG 3350, 5% PEG 400, 0.1 M Bis-Tris, pH 5.5, 10 mM DTT, and 10 mM L-cysteine, with or without ligands added as 10 mM AMP-PCP, 10 mM MgCl2, and 5 mM inositol-1,3,4-trisphosphate, direct X-ray diffraction analysis after this step or further seeding adding ADP and other ligands, overview, X-ray diffraction structure determination and analysis at 1.2-2.0 A resolution

Entamoeba histolytica