2.7.1.160: 2'-phosphotransferase
This is an abbreviated version!
For detailed information about 2'-phosphotransferase, go to the full flat file.
Word Map on EC 2.7.1.160
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2.7.1.160
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2'-phosphate
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junction
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adp-ribose
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adp-ribosylation
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2.7.1.23
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phosphodiester
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2'-phosphorylated
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pre-trnas
- 2.7.1.160
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2'-phosphate
- junction
- adp-ribose
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adp-ribosylation
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2.7.1.23
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phosphodiester
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2'-phosphorylated
- pre-trnas
Reaction
Synonyms
2'-phosphotransferase, ApeTpt1, AtPt, CTHT_0025470, HeLa cell 2'-phosphotransferase, human TRPT1, KptA, kptA1, mTPT1, NAD+-dependent 2'-phosphotransferase, NAD-dependent RNA 2'-phosphotransferase, RNA 2'-phosphotransferase, RslTpt1, Tpt1, Tpt1p, tRNA 2'-phosphotransferase, Trpt1, yeast 2'-phosphotransferase
ECTree
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Purification
Purification on EC 2.7.1.160 - 2'-phosphotransferase
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by Blue Sepharose column chromatography
His6-tagged wild-type RslTpt1 and mutant R16A/H17A/R64A/R119A RslTpt1 from Escherichia coli strain L21(DE3)-CodonPlus by nickel affinity chromatography, ultrafiltration, and gel filtration
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immobilized metal ion affinity chromatography, > 80% purified by SDS-PAGE
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KptA-His6 10% purified by sonication, immobilized metal ion chromatography, centrifugation
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Ni-agarose chromatography
partially purified
partially purified, GST-Tpt1 fusion protein purified by glutahthione agarose chromatography
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purified 28000fold by Orange A Sepharose column, 4fold less pure by streamlined purification procedure
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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Tpt1-His6 50% purified by sonication, immobilized metal ion chromatography, centrifugation
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recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Thermochaetoides thermophila
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis