2.7.1.190: aminoglycoside 2''-phosphotransferase

This is an abbreviated version!
For detailed information about aminoglycoside 2''-phosphotransferase, go to the full flat file.

Reaction

Synonyms

AAC(6')-APH(2''), AAC(6')-Ie/APH(2'')-Ia, aac6-aph2, aacA-aphD, AME, aminoglycoside (2'') kinase, aminoglycoside 2''-phosphotransferase IVa, aminoglycoside 2''-phosphotransferase type IIIa, aminoglycoside kinase, aminoglycoside phosphotransferase, aminoglycoside-2''-phosphotransferase, aminoglycoside-2''-phosphotransferase type IVa, aminoglycoside-modification enzyme, APH, APH(2''), APH(2'')-Ia, APH(2'')-Ia aminoglycoside resistance enzyme, APH(2'')-Id, Aph(2'')-If, APH(2'')-IIIa, APH(2'')-IVa, aphD, AphSR2, bifunctional 6'-aminoglycoside acetyltransferase/2"-aminoglycoside phosphotransferase, bifunctional AAC/APH, bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia, bifunctional aminoglycoside acetyltransferase/phosphotransferase, gentamicin kinase, gentamicin phosphotransferase, gentamicin resistance protein

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.190 aminoglycoside 2''-phosphotransferase

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Results	in table
264	AA Sequence
1	Application
7	Cloned(Commentary)
9	Cofactor
7	Crystallization (Commentary)
16	General Information
1	IC50 Value
38	Inhibitors
33	kcat/KM [mM/s]
11	Ki Value [mM]
38	KM Value [mM]
7	Metals/Ions
2	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
20	Organism
24	PDB ID
3	pH Optimum
10	Protein Variants
2	Purification (Commentary)
3	Reaction
17	Reference
70	Substrates and Products (Substrate)
4	Subunits
52	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
36	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 2.7.1.190 - aminoglycoside 2''-phosphotransferase

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SYSTEMATIC NAME

IUBMB Comments

GTP:gentamicin 2''-O-phosphotransferase

Requires Mg2+. This bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2'', including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin. In most, but not all, cases the phosphorylation confers resistance against the antibiotic. Some forms of the enzyme use ATP as a phosphate donor in appreciable amount. The enzyme is often found as a bifunctional enzyme that also catalyses 6'-aminoglycoside N-acetyltransferase activity. The bifunctional enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci.