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2.7.1.2: glucokinase

This is an abbreviated version!
For detailed information about glucokinase, go to the full flat file.

Word Map on EC 2.7.1.2

Reaction

ATP
+
D-glucose
=
ADP
 +
D-glucose 6-phosphate

Synonyms

ATP-dependent glucokinase, ATP-GLK, GK, GlcK, glk, Glk1, glucokinase (phosphorylating), glucokinase 1, kinase, gluco- (phosphorylating), KlGlk1, KLLA0C01155g, LmGlcK, NagC, NfGlck, SgGlkA, Sw0678, Sw2155, Sw3773, TcGlcK, TTE0090

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.2 glucokinase

Cloned

Cloned on EC 2.7.1.2 - glucokinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli. Expression of the gene is low resulting in a poor yield of the recombinant protein. A single site directed mutation in Pcal_1032 gene (Nucleotide G at position 11 is replaced by A), without altering the amino acid sequence, results in approximately tenfold higher expression
expression in Saccharomyces cerevisiae
expression of genes nanK, yajF, and ycfX in glk-deficient mutant strain BM5340(DE3)
-
gene glcK, phylogenetic analysis and tree, functional complementation of enzyme-deficient Escherichia coli strain UE26, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain RB791 as soluble proteins
gene glk is organized in gene clusters, transcriptomic analysis of KT2440 in response to glucose, overview
-
gene glk, expression of His-tagged enzyme in strain BL21(DE3)
gene sll0593, DNA and amino acid sequence determination
-
gene TM 1469 or glk, functional expression of the soluble His-tagged in Escherichia coli strain BL21(DE3)
-
gene TM 1469, expression in Escherichia coli strain BL21(DE3)
-
glcK open reading frame is cloned from Bacillus sphaericus strain C3-41 and then expressed in Escherichia coli
heterologous expression in Escherichia coli
heterologous expression in Escherichia coli BL21 (DE3) as a C-terminal His6 version in an IPTG-inducible expression vector
overexpression in Escherichia coli UE79
-
the KLLA0C01155g gene is codon optimized for improved expression in Escherichia coli. Expression in Escherichia coli BL21 (DE3)