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2.7.1.63: polyphosphate-glucose phosphotransferase

This is an abbreviated version!
For detailed information about polyphosphate-glucose phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.63

Reaction

(Phosphate)n
+
D-glucose
=
(Phosphate)n-1
 +
D-glucose 6-phosphate

Synonyms

All1371, GMK, inorganic polyphosphate/ATP-glucomannokinase, inorganic polyphosphate:D-glucose 6-phosphotransferase, More, PGPTase, phosphotransferase, polyphosphate-glucose, poly P glucokinase, poly(P) glucokinase, poly(P)/ATP-glucomannokinase, PolyP-dependent glucokinase, polyP-GK, polyP-Glk, polyphosphate glucokinase, polyphosphate-D-(+)-glucose-6-phosphotransferase, polyphosphate-dependent glucokinase, polyphosphate-glucose 6-phosphotransferase, polyphosphate/ATP-glucomannokinase, PP-Glk, PPGK, SCO5059

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.63 polyphosphate-glucose phosphotransferase

Temperature Stability

Temperature Stability on EC 2.7.1.63 - polyphosphate-glucose phosphotransferase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
2 h, 30% loss of activity
30 - 40
purified recombinant enzyme, 100% remaining activity after 1 h
35
2 h, 64% loss of activity
37
-
24 h, pH 4-5: precipitation and 80% of activity retained, above pH 5.5: inactivation within 24 h, 0.3 M KCl stabilizes
50
purified recombinant enzyme, 12% remaining activity after 1 h
55.9
Tm-value, wild-type enzyme
59.1
Tm-value, mutant enzyme A231T
60
purified recombinant enzyme, no remaining activity after 5 min
60 - 65
5 min, complete inactivation
65.2
Tm-value, mutant enzyme F30L/T72A/A231T
70.6
Tm-value, mutant enzyme F30L/T72A/V88M/A231T
75.7
Tm-value, mutant enzyme F30L/R34P/T72A/V88M/A231T