1.1.1.251: galactitol-1-phosphate 5-dehydrogenase

This is an abbreviated version!
For detailed information about galactitol-1-phosphate 5-dehydrogenase, go to the full flat file.

Reaction

galactitol 1-phosphate
+
NAD+
=
D-tagatose 6-phosphate
+
NADH
+
H+

Synonyms

galactitol-1-phosphate dehydrogenase (Escherichia coli strain EC3132 gene gatD), Gat1P-specific NAD-dependent dehydrogenase, GatD, Genbank X79837-derived protein, GPDH, NAD-dependent Gat1P-dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.251 galactitol-1-phosphate 5-dehydrogenase

Crystallization

Crystallization on EC 1.1.1.251 - galactitol-1-phosphate 5-dehydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
modeling of structure and docking analysis of substrate, Zn2+ and NAD+. Cys 37, His 58, Glu 59, Glu 142 residues form an active site pocket similar to known GPDH. A catalytic Zn2+-binding domain and a cofactor NAD+-binding domain with strong hydrogen bonding contacts with the substrate and the cofactor are identified
structures of open state with Zn2+ in the catalytic site and of the closed state in complex with the polyols Tris and glycerol, respectively. The closed state reveals no bound cofactor. The glycerol binding mode reveals a pentacoordinated zinc ion in complex with a polyol and also a strong hydrogen bond between the primary hydroxyl group and the conserved Glu144