1.13.11.61: linolenate 9R-lipoxygenase

This is an abbreviated version!
For detailed information about linolenate 9R-lipoxygenase, go to the full flat file.

Reaction

Synonyms

(9R)-LOX, 11R-LOX, 9R-lipoxygenase, 9R-LOX, ARA 11RLOX, arachidonate 11R-lipoxygenase, CspLOX2, LA 9RLOX, linoleate 11R-lipoxygenase, linoleate 9R-dioxygenase, lipoxygenase 2, NspLOX

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.61 linolenate 9R-lipoxygenase

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Results	in table
9	Activating Compound
7	AA Sequence
5	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
1	General Information
11	Inhibitors
6	kcat/KM [mM/s]
12	KM Value [mM]
1	Metals/Ions
2	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
29	Organism
1	Pathway
4	pH Optimum
2	pH Range
9	Protein Variants
4	Purification (Commentary)
2	Reaction
9	Reference
1	Source Tissue
6	Specific Activity [micromol/min/mg]
30	Substrates and Products (Substrate)
4	Subunits
23	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.13.11.61 - linolenate 9R-lipoxygenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant wild-type enzyme CspLOX2, sitting drop vapor diffusion method, from well solution of 10% PEG 4000, 0.1 M MES/imidazole pH 6.5, 20% glycerol and 0.02% alcohols (1,6-hexanediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol, and 1,3-propanediol), 10 days, purified recombinant CspLOX2 mutant enzymes by hanging drop vapour diffusion method, from well solution of 12.5-15 PEG 4000, 8-12% glycerol, 0.1 M MES/imidazole pH 6.1, and 0-600 mM NaCl, X-ray diffraction structure determination and analysis at 1.8 A resolution. Crystals of a CspLOX2 substrate complex are not obtained

Rippkaea orientalis PCC 8801

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