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1.14.14.95: germacrene A hydroxylase

This is an abbreviated version!
For detailed information about germacrene A hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.95

Reaction

(+)-germacrene A
+ 3 [reduced NADPH-hemoprotein reductase] + 3 O2 =
germacra-1(10),4,11(13)-trien-12-oate
 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O

Synonyms

(+)-germacrene A hydroxylase, EC 1.1.1.314, EC 1.14.13.123, GAO, germacrene A oxidase, NADP+-dependent sesquiterpenoid dehydrogenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.95 germacrene A hydroxylase

Cofactor

Cofactor on EC 1.14.14.95 - germacrene A hydroxylase

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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P-450
-
flavin
-
an assay buffer without flavins (FAD and FMN) gives 18% loss of hydroxylase activity. However, omitting these flavins from the extraction buffer results in a loss of more than 70% in enzyme activity
NADP+
-
dependent on