1.7.7.2: ferredoxin-nitrate reductase
This is an abbreviated version!
For detailed information about ferredoxin-nitrate reductase, go to the full flat file.
Reaction
Synonyms
assimilatory ferredoxin-nitrate reductase, Assimilatory nitrate reductase, C452_13229, dissimilatory nitrate reductase, EC 1.7.99.4, Fd-dependent nitrate reductase, Fd-NAS, NaR, narB, NarGH, NAS, NasB, nitrate (ferredoxin) reductase, nitrate reductase, reductase, nitrate (ferredoxin), respiratory nitrate reductase
ECTree
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Inhibitors
Inhibitors on EC 1.7.7.2 - ferredoxin-nitrate reductase
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ammonia
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after initial slight stimulation rapid and complete inhibition, promotes conversion of ferredoxin-nitrate reductase into its reduced inactive form, metabolic interconversion
KCl
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inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength
light
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light- and oxygen-dependent inactivation of enzyme activity in absence but not in presence of any added nitrogen source, inactivation may result from an oxidative modification of enzyme, assay in cell suspension
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MgSO4
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inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength
N-acetylsuccinimide
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115fold excess, loss of more than 80% and 5-10% loss of ferredoxin-dependent and methylviologen dependent activity after 1 h, respectively
NaCl
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more than 80% inhibition above 200 mM, inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength
oxygen
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light- and oxygen-dependent inactivation of enzyme activity in absence but not in presence of any added nitrogen source, inactivation may result from an oxidative modification of enzyme, assay in cell suspension
Phenylglyoxal
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76fold excess, approx. 60% and 20% loss of ferredoxin dependent and methylviologen dependent activity after 2 h, respectively
thiocyanate
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10 mM, 53% inhibition. Preincubating for 1 h does not increase the inhibition
cyanide
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preincubating the enzyme with cyanide increases the extent of inhibition which in turn is intensified when dithionite is present. Although cyanide is a noncompetitive inhibitor with respect to nitrate, nitrate protects against inhibition
Dithionite
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inactivation can be suppressed by cyanide and azide and specifically prevented by superoxide dismutase
Dithionite
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converts native enzyme in modified form without ferredoxin-linked activity but retained methyl viologen-linked activity
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nitrate plays an active role in enzyme synthesis, ammonia acts as antagonist
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additional information
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not inhibited by 0.1 mM p-chloromercuribenzoate, enzyme formation is induced by nitrate and inhibited by 0.5 mM tungstate, but recovered by 0.1 mM molybdate
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additional information
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not inhibited by potassium chlorate, dithiothreitol, p-hydroxymercuribenzoate, sulfite
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additional information
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nitrate plays an active role in enzyme synthesis, ammonia acts as antagonist
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additional information
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enzyme synthesis is repressed in presence of ammonia as a result of its metabolism, nitrate is not required as an obligate inducer
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