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EC 2.9.1.3 Details

show Brenda entry

EC number

2.9.1.3

Accepted name

tRNA 2-selenouridine synthase

Reaction

selenophosphate + geranyl diphosphate + 5-methylaminomethyl-2-thiouridine³⁴ in tRNA + H₂O = 5-methylaminomethyl-2-selenouridine³⁴ in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol + diphosphate + phosphate (overall reaction);;(1a) geranyl diphosphate + 5-methylaminomethyl-2-thiouridine³⁴ in tRNA = 5-methylaminomethyl-2-(S-geranyl)thiouridine³⁴ in tRNA + diphosphate;;(1b) selenophosphate + 5-methylaminomethyl-2-(S-geranyl)thiouridine³⁴ in tRNA = 5-methylaminomethyl-2-(Se-phospho)selenouridine³⁴ in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol;;(1c) 5-methylaminomethyl-2-(Se-phospho)selenouridine³⁴ in tRNA + H₂O = 5-methylaminomethyl-2-selenouridine³⁴ in tRNA + phosphate

Other name(s)

selU (gene name), mnmH (gene name), ybbB (gene name), sufY (gene name)

Systematic name

geranyl diphosphate/selenophosphate:tRNA 5-methylaminomethyl-2-thiouridine³⁴ geranyl/selenophosphatetransferase

Comment

This bacterial enzyme converts 5-methylaminomethyl-2-uridine and 5-carboxymethylaminomethyl-2-uridine to the respective selenouridine forms in a two-step process that involves geranylation and subsequent phosphoselenation of the resulting geranylated intermediates. The resultant seleno-phosphorylated uridine intermediates further react with a water molecule to release a phosphate anion and 2-selenouridine tRNA. The enzyme contains a rhodanese domain.

History

created 2020

EC Tree

1 Oxidoreductases

2 Transferases

2.1 Transferring one-carbon groups

2.2 Transferring aldehyde or ketonic groups

2.3 Acyltransferases

2.4 Glycosyltransferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.6 Transferring nitrogenous groups

2.7 Transferring phosphorus-containing groups

2.8 Transferring sulfur-containing groups

2.8.1 Sulfurtransferases

2.8.2 Sulfotransferases

2.8.3 CoA-transferases

2.8.3.1 propionate CoA-transferase

2.8.3.2 oxalate CoA-transferase

2.8.3.3 malonate CoA-transferase

2.8.3.4 created 1961, deleted 1964

2.8.3.5 3-oxoacid CoA-transferase

2.8.3.6 3-oxoadipate CoA-transferase

2.8.3.7 succinate—citramalate CoA-transferase

2.8.3.8 acetate CoA-transferase

2.8.3.9 butyrate—acetoacetate CoA-transferase

2.8.3.10 citrate CoA-transferase

2.8.3.11 citramalate CoA-transferase

2.8.3.12 glutaconate CoA-transferase

2.8.3.13 succinate—hydroxymethylglutarate CoA-transferase

2.8.3.14 5-hydroxypentanoate CoA-transferase

2.8.3.15 succinyl-CoA:(R)-benzylsuccinate CoA-transferase

2.8.3.16 formyl-CoA transferase

2.8.3.17 3-(aryl)acryloyl-CoA:(R)-3-(aryl)lactate CoA-transferase

2.8.3.18 succinyl-CoA:acetate CoA-transferase

2.8.3.19 CoA:oxalate CoA-transferase

2.8.3.20 succinyl-CoA—D-citramalate CoA-transferase

2.8.3.21 L-carnitine CoA-transferase

2.8.3.22 succinyl-CoA—L-malate CoA-transferase

2.8.3.23 caffeate CoA-transferase

2.8.3.24 (R)-2-hydroxy-4-methylpentanoate CoA-transferase

2.8.3.25 bile acid CoA-transferase

2.8.3.26 succinyl-CoA:mesaconate CoA transferase

2.8.3.27 propanoyl-CoA:succinate CoA transferase

2.8.3.28 phenylsuccinyl-CoA transferase

2.8.4 Transferring alkylthio groups

2.8.5 Thiosulfotransferases

2.9 Transferring selenium-containing groups

2.9.1 Selenotransferases

2.9.1.1 L-seryl-tRNA^Sec selenium transferase

2.9.1.2 O-phospho-L-seryl-tRNA^Sec:L-selenocysteinyl-tRNA synthase

2.9.1.3 tRNA 2-selenouridine synthase

2.10 Transferring molybdenum- or tungsten-containing groups