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EC Tree
IUBMB Comments Specific for prostaglandins D [cf. EC 1.1.1.141 15-hydroxyprostaglandin dehydrogenase (NAD+) and EC 1.1.1.197 15-hydroxyprostaglandin dehydrogenase (NADP+)].
The enzyme appears in viruses and cellular organisms
Synonyms
prostaglandin d2 dehydrogenase, nadp+-dependent 15-hydroxyprostaglandin dehydrogenase,
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15-hydroxy PGD2 dehydrogenase
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15-hydroxy prostaglandin dehydrogenase (NADP+)
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dehydrogenase, 15-hydroxyprostaglandin (nicotinamide adenine dinucleotide phosphate)
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dehydrogenase, prostaglandin D2
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NADP+-dependent 15-hydroxyprostaglandin dehydrogenase
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NADP+-linked 15-hydroxyprostaglandin dehydrogenase
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NADP+-linkedprostaglandin D2 dehydrogenase
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NADP+-specific 15-hydroxyprostaglandindehydrogenase
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NADP-PGD2 dehydrogenase
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prostaglandin D2 dehydrogenase
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prostaglandin-D 15-dehydrogenase (NADP+)
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(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADP+ = (5Z,13E)-9alpha-hydroxy-11,15-dioxoprosta-5,13-dienoate + NADPH + H+
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(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate:NADP+ 15-oxidoreductase
Specific for prostaglandins D [cf. EC 1.1.1.141 15-hydroxyprostaglandin dehydrogenase (NAD+) and EC 1.1.1.197 15-hydroxyprostaglandin dehydrogenase (NADP+)].
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15-keto-DELTA13-prostaglandin + NADP+
? + NADPH
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?
prostaglandin A2 + NADP+
15-ketoprostaglandin A2 + NADPH + H+
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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?
prostaglandin B2 + NADP+
15-ketoprostaglandin B2 + NADPH + H+
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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?
prostaglandin D1 + NADP+
15-ketoprostaglandin D1 + NADPH + H+
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
prostaglandin D3 + NADP+
15-ketoprostaglandin D3 + NADPH + H+
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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?
prostaglandin E2 + NADP+
15-ketoprostaglandin E2 + NADPH + H+
prostaglandin F2alpha + NADP+
15-ketoprostaglandin F2alpha + NADPH + H+
prostaglandin I2 + NADP+
15-ketoprostaglandin I2 + NADPH + H+
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
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specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
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specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
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?
prostaglandin E2 + NADP+
15-ketoprostaglandin E2 + NADPH + H+
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?
prostaglandin E2 + NADP+
15-ketoprostaglandin E2 + NADPH + H+
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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?
prostaglandin F2alpha + NADP+
15-ketoprostaglandin F2alpha + NADPH + H+
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?
prostaglandin F2alpha + NADP+
15-ketoprostaglandin F2alpha + NADPH + H+
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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?
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prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
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specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
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specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
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?
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NAD+
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activity less than 1% of that with NADP+
NADP+
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strict specificity for
NADP+
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utilizes this cofactor exclusively
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11,14-Eicosadienoic acid
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3,3',5-Triiodothyroacetic acid
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p-chloromercuribenzoate
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Myeloproliferative Disorders
Platelet prostaglandin D2 dehydrogenase in patients with myeloproliferative disorders.
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0.278
prostaglandin D1
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0.052 - 0.07
Prostaglandin D2
0.35
prostaglandin E2
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0.4
prostaglandin F2alpha
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0.54
prostaglandin I2
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0.0005
NADP+
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0.07
Prostaglandin D2
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0.052
Prostaglandin D2
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9.5
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optimal for reaction with prostaglandins D1, D2, E2 and I2
9.5 - 10
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with prostaglandin F2alpha as substrate
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human
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brenda
swine
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brenda
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platelet
brenda
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brenda
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brenda
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CBR1_BOVIN
277
0
30533
Swiss-Prot
other Location (Reliability: 5 )
CBR1_HUMAN
277
0
30375
Swiss-Prot
other Location (Reliability: 4 )
CBR1_MOUSE
277
0
30641
Swiss-Prot
Mitochondrion (Reliability: 5 )
CBR1_PIG
289
0
31693
Swiss-Prot
Mitochondrion (Reliability: 3 )
CBR1_PONAB
277
0
30391
Swiss-Prot
other Location (Reliability: 5 )
CBR1_RABIT
277
0
30452
Swiss-Prot
Mitochondrion (Reliability: 4 )
CBR1_RAT
277
0
30578
Swiss-Prot
other Location (Reliability: 4 )
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30000
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sedimentation equilibrium
52000 - 62000
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gel filtration
28000
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gel electrophoresis
28000
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2 * 28000, SDS-PAGE
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dimer
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2 * 28000, SDS-PAGE
monomer
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1 * 28000, SDS-PAGE
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7
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most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9
347826
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enzyme is quite unstable at the low protein concentrations less than 10 mg/ml, sulfhydryl reagents like dithiothreitol, 2-mercaptoethanol and reduced glutathione protect the enzyme from inactivation
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4°C, at pH 7 for 6 h, 54% loss of enzyme activity
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Watanabe, K.; Shimizu, T.; Iguchi, S.; Wakatsuka, H.; Hayashi, M.; Hayaishi, O.
An NADP-linked prostaglandin D dehydrogenase in swine brain
J. Biol. Chem.
255
1779-1782
1980
Sus scrofa
brenda
Shimizu, T.; Watanabe, K.; Tokumoto, H.; Hayaishi, O.
Isolation of NADP+-dependent PGD2-specific 15-hydroxyprostaglandin dehydrogenase from swine brain
Methods Enzymol.
86
147-152
1982
Sus scrofa
brenda
Tokumoto, H.; Watanabe, K.; Fukushima, D.; Shimizu, T.; Hayaishi, O.
An NADP-linked 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin D2 from swine brain
J. Biol. Chem.
257
13576-13580
1982
Homo sapiens, Sus scrofa
brenda
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1.1.1.196 15-hydroxyprostaglandin-D dehydrogenase (NADP+)
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