Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-(trifluoromethyl)benzyl alcohol + NADH + H+
?
-
93% ethanoselective reaction
-
-
r
(R)-1,3-butanediol + NAD+
?
-
-
-
-
r
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
(R)-2-butanol + NAD+
2-butanone + NADH + H+
(R)-2-pentanol + NAD+
2-pentanone + NADH + H+
-
-
-
-
r
(R)-adrenaline + NAD+
? + NADH + H+
-
-
-
-
r
(S)-1,3-butanediol + NAD+
?
-
-
-
-
r
1,2-butanediol + NAD+
? + NADH
-
20% of the activity with 2-propanol
-
-
r
1,2-propanediol + NAD+
? + NADH
-
45% of the activity with 2-propanol
-
-
r
1,3-butanediol + NAD+
? + NADH
-
126% of the activity with 2-propanol
-
-
r
1-(2-phenylcyclopropyl)ethanone + NADH
(1R)-1-(2-phenylcyclopropyl)ethanol + NAD+
1-(2-pyridyl)acetone + NADPH + H+
1-(2-pyridyl)ethanol + NADP+
-
-
-
-
r
1-(2-thienyl)acetone + NADPH + H+
1-(2-thienyl)ethanol + NADP+
-
-
-
-
r
1-(3-pyridyl)acetone + NADPH + H+
1-(3-pyridyl)ethanol + NADP+
-
-
-
-
r
1-(3-thienyl)acetone + NADPH + H+
1-(3-thienyl)ethanol + NADP+
-
-
-
-
r
1-(4-pyridyl)acetone + NADPH + H+
1-(4-pyridyl)ethanol + NADP+
-
-
-
-
r
1-butanol + NAD+
butanal + NADH
1-chloropentane-2,4-dione + NADH
(4R)-5-chloro-4-hydroxypentan-2-one + NAD+
1-phenoxypropan-2-one + NADH
(2R)-1-phenoxypropan-2-ol + NAD+
1-phenyl-1,2-propandione + NADPH + H+
? + NADP+
1-phenylethanone + NADH
(1R)-1-phenylethanol + NAD+
1-phenylpropane-1,2-dione + NADH
(1S)-1-hydroxy-1-phenylpropan-2-one + NAD+
1-phenylpropane-1,2-dione + NADH
? + NAD+
-
-
-
-
r
1-phenylpropanone + NADPH + H+
1-phenylpropanol + NADP+
-
-
-
-
r
1-propanol + NAD+
propanal + NADH
-
9% of the activity with 2-propanol
-
-
r
2',4'-difluoro-acetophenone + NADPH + H+
1-(2',4'-difluoro)-phenylethanol + NADP+
-
-
-
-
r
2'-methylacetophenone + NADPH + H+
1-(2'-methyl)phenylethanol + NADP+
-
-
-
-
r
2,2,2-trifluoro-1-phenylethanone + NADH + H+
(1R)-2,2,2-trifluoro-1-phenylethanol + NAD+
-
-
21% of the activity with 1-phenylpropane-1,2-dione
-
r
2,2,2-trifluoro-1-phenylethanone + NADH + H+
(1S)-2,2,2-trifluoro-1-phenylethanol + NAD+
-
-
37% yield, 92% enantiomeric excess
-
?
2,2,2-trifluoroacetophenone + NADH + H+
(1R)-2,2,2-trifluoro-1-phenylethanol + NAD+
-
completely enantioselective reaction
-
-
r
2,3-butanediol + NAD+
? + NADH
2,3-butanedione + NADPH + H+
(R)-2-hydroxy-3-oxo-butane + NADP+
2,3-pentanedione + NADPH + H+
(R)-2-hydroxy-3-oxo-pentane + NADP+
-
-
-
-
?
2-amino-3',4'-dihydroxyacetophenone + NADH + H+
? + NAD+
-
-
-
-
r
2-amino-4'-amino-acetophenone + NADH + H+
? + NAD+
-
-
-
-
r
2-amino-4'-hydroxyacetophenone + NADH + H+
? + NAD+
-
-
-
-
r
2-amino-acetophenone + NADH + H+
(R)-2-amino-1-phenylethanol + NAD+
-
-
-
-
r
2-amino-acetophenone + NADH + H+
? + NAD+
-
-
-
-
r
2-bromoacetophenone + NADH + H+
? + NAD+
-
-
-
-
r
2-butanol + NAD+
2-butanone + NADH
-
(R)-isomer is preferred over (S)-isomer
-
-
r
2-butanone + NAD+
2-butanol + NADH + H+
-
274% of the activity with beta-hydroxyacetophenone
-
-
?
2-butanone + NADH
(S)-2-hydroxybutane + NAD+
2-butanone + NADH
2-butanol + NAD+
-
83% of the activity with acetone
-
-
r
2-heptanone + NAD+
2-heptanol + NADH + H+
-
281% of the activity with beta-hydroxyacetophenone
-
-
?
2-hexanone + NAD+
2-hexanol + NADH + H+
-
259% of the activity with beta-hydroxyacetophenone
-
-
?
2-octanone + NAD+
2-octanol + NADH + H+
-
350% of the activity with beta-hydroxyacetophenone
-
-
?
2-pentanol + NAD+
2-pentanone + NADH
2-pentanone + NAD+
2-pentanol + NADH + H+
-
249% of the activity with beta-hydroxyacetophenone
-
-
?
2-pentanone + NADH + H+
(S)-2-hydroxypentane + NAD+
-
6% of the activity with 2-butanone
-
-
r
2-pentanone + NADH + H+
2-pentanol + NAD+
-
44% of the activity with acetone
-
-
r
2-propanol + NAD+
acetone + NADH
2-propanol + NAD+ + H+
acetone + NADH
-
-
-
-
r
3'-bromo-acetophenone + NADPH + H+
1-(3'-bromo-)phenylethanol + NADP+
-
-
-
-
r
3'-chloroacetophenone + NADPH + H+
1-(3'-chlorophenyl)ethanol + NADP+
-
-
-
-
r
3'-iodoacetophenone + NADPH + H+
1-(3'-iodophenyl)ethanol + NADP+
-
-
-
-
r
3'-methoxyacetophenone + NADPH + H+
1-(3'-methoxyphenyl)ethanol + NADP+
-
-
-
-
r
3'-methylacetophenone + NADPH + H+
1-(3'-methylphenyl)ethanol + NADP+
-
-
-
-
r
3'-trifluoromethyl-acetophenone + NADPH + H+
1-[(3'-trifluoromethyl)phenyl]ethanol + NADP+
-
-
-
-
r
3'-trifluoromethylacetophenone + NADPH + H+
1-[(3'-trifluoromethyl)phenyl]ethanol + NADP+
-
-
-
-
r
3,5-bis(trifluoromethyl)acetophenone + NADH + H+
(R)-[3,5-bis(trifluoromethyl)phenyl]ethanol + NAD+
3-(dimethylamino)-1-phenylpropan-1-one + NADH
(1S)-3-dimethylamino-1-phenylpropan-1-ol + NAD+
-
-
24% of the activity with 1-phenylpropane-1,2-dione
-
r
3-chloro-pentane-2,4-dione + NADH
(4R)-3-chloro-4-hydroxypentan-2-one + NAD+
-
336% of the activity with 2-butanone
-
-
r
3-hexanone + NAD+
3-hexanol + NADH + H+
-
29% of the activity with beta-hydroxyacetophenone
-
-
?
3-pentanol + NAD+
3-pentanone + NADH
-
23% of the activity with 2-propanol
-
-
r
3-pentanone + NAD+
3-pentanol + NADH + H+
-
35% of the activity with beta-hydroxyacetophenone
-
-
?
3-pentanone + NADH
3-pentanol + NAD+
-
16% of the activity with acetone
-
-
r
4-hydroxy-2-butanone + NADH
? + NAD+
-
95% of the activity with acetone
-
-
r
4-methylpentan-2-one + NADH + H+
4-methylpentan-2-ol + NAD+
4-phenylbutan-2-one + NADH
(2R)-4-phenylbutan-2-ol + NAD+
-
-
18% of the activity with 1-phenylpropane-1,2-dione
-
r
4-phenylbutan-2-one + NADPH + H+
(2R)-4-phenylbutan-2-ol + NADP+
-
-
99% conversion, 97.9% enantiomeric excess
-
?
5-chloropentan-2-one + NADH
(2S)-5-chloropentan-2-ol + NAD+
-
-
48% yield, 93% enantiomeric excess
-
?
6-methylhept-5-en-2-one + NADH
(2S)-6-methylhept-5-en-2-ol + NAD+
-
-
51% yield, 97% enantiomeric excess
-
?
acetaldehyde + NAD+
ethanol + NADH + H+
-
-
-
-
r
acetone + NAD+
isopropanol + NADH + H+
-
260% of the activity with beta-hydroxyacetophenone
-
-
?
acetone + NADH
2-propanol + NAD+
-
-
-
-
r
acetone + NADH + H+
propan-2-ol + NAD+
-
-
-
-
r
acetone + NADPH + H+
2-propanol + NADP+
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
-
-
-
r
acetophenone + NADH + H+
phenylethanol + NAD+
acetophenone + NADPH + H+
(1R)-1-phenylethanol + NADP+
-
-
-
?
acetophenone + NADPH + H+
1-phenylethanol + NADP+
-
-
-
-
r
acetophenone + NADPH + H+
phenylethanol + NADP+
-
-
-
-
?
adrenalone + NADH + H+
(R)-epinephrine + NAD+
-
the NAD(H)-dependent dehydrogenase catalyzes the asymmetric reduction of adrenalone (corticosterone) to (R)-epinephrine, with an enantiomeric excess (e.e value) of more than 99%
-
-
r
adrenosterone + NADH + H+
? + NAD+
-
-
-
-
r
benzoylformic acid + NADH + H+
? + NAD+
-
-
-
-
r
beta-hydroxyacetophenone + NAD+
(R)-1-phenyl-1,2-ethanediol + NADH + H+
-
-
formation of (R)-enantiomner with 100% enantiomeric excess
-
?
cycloheptanone + NADH + H+
cycloheptanol + NAD+
ephedrine + NAD+
1-(3,4-dihydroxyphenyl)-2-(methylamino)-propan-1-one + NADH + H+
-
-
-
-
r
ethanol + NAD+
ethanal + NADH
ethyl (R)-mandelate + NADH + H+
ethyl benzoylformate + NAD+
-
95% ethanoselective reaction
-
-
r
ethyl 2-oxo-3-phenylpropanoate + NADH + H+
ethyl 2-hydroxy-3-phenylpropanoate + NAD+
48% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
ethyl 2-oxo-4-phenylbutanoate + NADH + H+
ethyl 2-hydroxy-4-phenylbutanoate + NAD+
135% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+
ethyl (2S)-2-hydroxy-4-phenylbutanoate + NADP+
ethyl 2-oxopropanoate + NADH + H+
ethyl 2-hydroxypropanoate + NAD+
-
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
ethyl 3-oxohexanoate + NADH + H+
ethyl 3-hydroxyhexanoate + NAD+
105% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+
ethyl 4-phenyl-2-oxobutanoate + NADH + H+
ethyl (R)-4-phenyl-2-hydroxybutanoate
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
ethyl oxo(phenyl)acetate + NADH + H+
ethyl hydroxy(phenyl)acetate + NAD+
270% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
ethyl oxo(phenyl)acetate + NADPH + H+
ethyl (2S)-hydroxy(phenyl)acetate + NADP+
-
-
-
-
?
formaldehyde + NADH + H+
methanol + NAD+
-
-
-
-
r
hydroxyacetone + NADH
? + NAD+
-
92% of the activity with acetone
-
-
r
isoproterenol + NAD+
1-(3,4-dihydroxyphenyl)-2-[(propan-2-yl)amino]ethane-1-one + NADH + H+
-
-
-
-
r
methyl (R)-mandelate + NADH + H+
methyl benzoylformate + NAD+
-
92% ethanoselective reaction
-
-
r
methyl 3-oxobutanoate + NADH
methyl (3S)-3-hydroxybutanoate + NAD+
-
135% of the activity with 2-butanone
-
-
r
methyl benzoylformate + NADH + H+
methyl (R)-mandelate + NAD+
-
completely enantioselective reaction
92% enantiomeric excess after 6 h at 50°C
-
r
methyl oxo(phenyl)acetate + NADH
methyl (2S)-hydroxy(phenyl)ethanoate + NAD+
-
-
79% yield, 98% enantiomeric excess
-
?
methylpyruvate + NADH
? + NAD+
-
64% of the activity with acetone
-
-
r
norepinephrine + NAD+
? + NADH + H+
-
-
-
-
r
pentane-2,4-dione + NADH
(4R)-4-hydroxypentan-2-one + NAD+
-
32% of the activity with 2-butanone
-
-
r
phenylephrine + NAD+
1-(3-hydroxyphenyl)-2-(methylamino)ethane-1-one + NADH + H+
-
-
-
-
r
additional information
?
-
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
r
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
r
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
?
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
-
-
-
-
?
(R)-1-phenylethanol + NAD+
acetophenone + NADH + H+
activity of mutant I886A, the wild-type enzyme is (S)-specific forming (S)-1-phenylethanol
-
-
r
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
r
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
r
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
(R)-2-butanol + NAD+
2-butanone + NADH + H+
-
-
-
-
?
1-(2-phenylcyclopropyl)ethanone + NADH
(1R)-1-(2-phenylcyclopropyl)ethanol + NAD+
-
-
18% of the activity with 1-phenylpropane-1,2-dione
-
r
1-(2-phenylcyclopropyl)ethanone + NADH
(1R)-1-(2-phenylcyclopropyl)ethanol + NAD+
-
-
18% of the activity with 1-phenylpropane-1,2-dione
-
r
1-butanol + NAD+
butanal + NADH
-
11% of the activity with 2-propanol
-
-
r
1-butanol + NAD+
butanal + NADH
-
11% of the activity with 2-propanol
-
-
r
1-chloropentane-2,4-dione + NADH
(4R)-5-chloro-4-hydroxypentan-2-one + NAD+
-
-
76% yield, 98% enantiomeric excess
-
?
1-chloropentane-2,4-dione + NADH
(4R)-5-chloro-4-hydroxypentan-2-one + NAD+
-
-
76% yield, 98% enantiomeric excess
-
?
1-phenoxypropan-2-one + NADH
(2R)-1-phenoxypropan-2-ol + NAD+
-
-
21% of the activity with 1-phenylpropane-1,2-dione
-
r
1-phenoxypropan-2-one + NADH
(2R)-1-phenoxypropan-2-ol + NAD+
-
-
21% of the activity with 1-phenylpropane-1,2-dione
-
r
1-phenyl-1,2-propandione + NADPH + H+
? + NADP+
-
-
-
-
?
1-phenyl-1,2-propandione + NADPH + H+
? + NADP+
-
-
-
-
?
1-phenylethanone + NADH
(1R)-1-phenylethanol + NAD+
-
-
34% yield, 94% enantiomeric excess
-
?
1-phenylethanone + NADH
(1R)-1-phenylethanol + NAD+
-
-
34% yield, 94% enantiomeric excess
-
?
1-phenylpropane-1,2-dione + NADH
(1S)-1-hydroxy-1-phenylpropan-2-one + NAD+
-
-
83% yield, 86% enantiomeric excess
-
?
1-phenylpropane-1,2-dione + NADH
(1S)-1-hydroxy-1-phenylpropan-2-one + NAD+
-
-
83% yield, 86% enantiomeric excess
-
?
2,3-butanediol + NAD+
? + NADH
-
73% of the activity with 2-propanol
-
-
r
2,3-butanediol + NAD+
? + NADH
-
73% of the activity with 2-propanol
-
-
r
2,3-butanedione + NADPH + H+
(R)-2-hydroxy-3-oxo-butane + NADP+
-
-
-
-
?
2,3-butanedione + NADPH + H+
(R)-2-hydroxy-3-oxo-butane + NADP+
-
-
-
-
?
2-butanone + NADH
(S)-2-hydroxybutane + NAD+
-
-
-
-
?
2-butanone + NADH
(S)-2-hydroxybutane + NAD+
-
-
-
-
r
2-pentanol + NAD+
2-pentanone + NADH
-
46% of the activity with 2-propanol
-
-
r
2-pentanol + NAD+
2-pentanone + NADH
-
46% of the activity with 2-propanol
-
-
r
2-propanol + NAD+
acetone + NADH
-
(R)-isomer is preferred over (S)-isomer
-
-
r
2-propanol + NAD+
acetone + NADH
-
(R)-isomer is preferred over (S)-isomer
-
-
r
3,5-bis(trifluoromethyl)acetophenone + NADH + H+
(R)-[3,5-bis(trifluoromethyl)phenyl]ethanol + NAD+
-
-
-
?
3,5-bis(trifluoromethyl)acetophenone + NADH + H+
(R)-[3,5-bis(trifluoromethyl)phenyl]ethanol + NAD+
-
-
-
?
4-methylpentan-2-one + NADH + H+
4-methylpentan-2-ol + NAD+
46% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
4-methylpentan-2-one + NADH + H+
4-methylpentan-2-ol + NAD+
46% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
acetophenone + NADH + H+
phenylethanol + NAD+
-
171% of the activity with beta-hydroxyacetophenone
-
-
?
acetophenone + NADH + H+
phenylethanol + NAD+
-
-
-
-
?
cycloheptanone + NADH + H+
cycloheptanol + NAD+
69% of the activity with ethyl 2-oxopropanoate
-
-
?
cycloheptanone + NADH + H+
cycloheptanol + NAD+
69% of the activity with ethyl 2-oxopropanoate
-
-
?
ethanol + NAD+
ethanal + NADH
-
4% of the activity with 2-propanol
-
-
r
ethanol + NAD+
ethanal + NADH
-
4% of the activity with 2-propanol
-
-
r
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+
ethyl (2S)-2-hydroxy-4-phenylbutanoate + NADP+
-
-
93% conversion, 99% enantiomeric excess
-
?
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+
ethyl (2S)-2-hydroxy-4-phenylbutanoate + NADP+
-
-
93% conversion, 99% enantiomeric excess
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
129% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
ethyl 4-chloro-3-oxobutanoate + NADH + H+
ethyl 4-chloro-3-hydroxybutanoate + NAD+
129% of the activity with ethyl 2-oxopropanoate
chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
73.4% conversion, 99% enantiomeric excess
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
73.4% conversion, 99% enantiomeric excess
-
?
ethyl 4-phenyl-2-oxobutanoate + NADH + H+
ethyl (R)-4-phenyl-2-hydroxybutanoate
enzyme catalyzes NAD(P)H-dependent asymmetric reduction to the (R)-enantiomer
product is a precursor of angiotensin-converting enzyme inhibitors such as cilazapril and benazepril
-
?
ethyl 4-phenyl-2-oxobutanoate + NADH + H+
ethyl (R)-4-phenyl-2-hydroxybutanoate
enzyme catalyzes NAD(P)H-dependent asymmetric reduction to the (R)-enantiomer
product is a precursor of angiotensin-converting enzyme inhibitors such as cilazapril and benazepril
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
270% of the activity with ethyl 2-oxopropanoate
95% conversion with 99.9% enantiomeric excess
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
270% of the activity with ethyl 2-oxopropanoate
95% conversion with 99.9% enantiomeric excess
-
?
ethyl benzoylformate + NADH + H+
ethyl (R)-mandelate + NAD+
-
completely enantioselective reaction
95% enantiomeric excess after 6 h at 50°C
-
r
additional information
?
-
enzyme exhibits reductase activity towards a broad spectrum of substrates including aliphatic linear, branched, and cyclic ketones, aromatic ketones, alpha-ketoesters, and beta-ketoesters. Both alpha- and beta-ketoesters serve as better substrates than ketones, but the enzymes shows a preference for alpha-ketoesters
-
-
?
additional information
?
-
enzyme exhibits reductase activity towards a broad spectrum of substrates including aliphatic linear, branched, and cyclic ketones, aromatic ketones, alpha-ketoesters, and beta-ketoesters. Both alpha- and beta-ketoesters serve as better substrates than ketones, but the enzymes shows a preference for alpha-ketoesters
-
-
?
additional information
?
-
-
the enzyme does not obey Prelog's rule and exhibits anti-Prelog enantiopreference. Hydride transfer occurs at the si faces of carbonyl group for ethyl 4-chloro-3-oxobutanoate (COBE), which is then selectively reduced to the chiral (S)-alcohol. Aromatic ketones are reduced to (R)-enantiomers, whereas keto esters are reduced to (S)-hydroxy esters with different enantioselectivities
-
-
?
additional information
?
-
-
the enzyme does not obey Prelog's rule and exhibits anti-Prelog enantiopreference. Hydride transfer occurs at the si faces of carbonyl group for ethyl 4-chloro-3-oxobutanoate (COBE), which is then selectively reduced to the chiral (S)-alcohol. Aromatic ketones are reduced to (R)-enantiomers, whereas keto esters are reduced to (S)-hydroxy esters with different enantioselectivities
-
-
?
additional information
?
-
-
the enzyme catalyzes transformation of aromatic beta-amino ketones to the corresponding chiral alcohols. The purified enzyme yields pure (R)-enantiomer product with high activity and utilizes NADH as the cofactor. The enzyme shows selectivity for many aromatic beta-amino ketones/alcohols such as 2-amino-acetophenone, 2-amino-4'-hydroxyacetophenone, isoproterenol, and ephedrine. Substrate specificity, overview. No or poor activity with L-Tyr, L-Phe, Trp, ethanol, methanol, acetanilide, ethalacetoacetate, 2-phenethyl alcohol, and phenylmethanol
-
-
?
additional information
?
-
-
the enzyme catalyzes stereoselective oxidation of (R)-secondary alcohols to corresponding ketones
-
-
?
additional information
?
-
-
the enzyme catalyzes stereoselective oxidation of (R)-secondary alcohols to corresponding ketones
-
-
?
additional information
?
-
-
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is capable for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (R)-phenylethanediol with in situ NADH regeneration and recycling, reusable system, method overview
-
-
?
additional information
?
-
-
the enzyme is coupled with formate dehydrogenase and co-immobilized on SiO2 particles, the system is capable for continuous catalytic conversion of beta-hydroxyacetophenone to optically pure (R)-phenylethanediol with in situ NADH regeneration and recycling, reusable system, method overview
-
-
?
additional information
?
-
-
the enzyme mutant I86A/C295A SADH gives high conversions and very high enantiomeric excess of the anti-Prelog R-alcohols from the tested substrates, it shows broad substrate specificity for meta-substituted, but not para-substituted, acetophenones and aryl ketones
-
-
?
additional information
?
-
-
substrate specificity of ADHTt in the oxidation and the reduction reactions depends on the substrate, cf. (S)-1-phenylethanol dehydrogenase, detailed overview. The enzyme shows a high reduction rate with halogenated aryl ketones, such as 2,2,2-trifluoroacetophenone, 2-chloroacetophenone, and 4-chlorobutyrophenone, and with aryl diketones, such as 1-phenyl-1,2-propanedione, although it is not active on benzil, i.e. diphenylethanedione. ADHTt proves to be very effective in reducing aryl alpha-keto esters, although it is not active on aliphatic alpha-keto esters and aryl beta-keto ester. Critical role of the D37 residue in discriminating NAD(H) from NADP(H)
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Pennacchio, A.; Pucci, B.; Secundo, F.; La Cara, F.; Rossi, M.; Raia, C.A.
Purification and characterization of a novel recombinant highly enantioselective short-chain NAD(H)-dependent alcohol dehydrogenase from Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
Thermus thermophilus
brenda
Isobe, K.; Wakao, N.
Thermostable NAD+-dependent (R)-specific secondary alcohol dehydrogenase from cholesterol-utilizing Burkholderia sp. AIU 652
J. Biosci. Bioeng.
96
387-393
2003
Burkholderia sp., Burkholderia sp. AIU 652
brenda
Bradshaw, C.W.; Fu, H.; Shen, G.-J.; Wong, C.-H
A Pseudomonas sp. alcohol dehydrogenase with broad substrate specificity and unusual stereospecificity for organic synthesis
J. Org. Chem.
57
1526-1532
1992
Pseudomonas sp., Pseudomonas sp. PED
-
brenda
Nie, Y.; Xu, Y.; Yang, M.; Mu, X.Q.
A novel NADH-dependent carbonyl reductase with unusual stereoselectivity for (R)-specific reduction from an (S)-1-phenyl-1,2-ethanediol-producing micro-organism: purification and characterization
Lett. Appl. Microbiol.
44
555-562
2007
Candida parapsilosis
brenda
Pal, S.; Park, D.H.; Plapp, B.V.
Activity of yeast alcohol dehydrogenases on benzyl alcohols and benzaldehydes: characterization of ADH1 from Saccharomyces carlsbergensis and transition state analysis
Chem. Biol. Interact.
178
16-23
2009
Saccharomyces cerevisiae, Saccharomyces pastorianus, Saccharomyces pastorianus Y379-50
brenda
Pennacchio, A.; Esposito, L.; Zagari, A.; Rossi, M.; Raia, C.A.
Role of Tryptophan 95 in substrate specificity and structural stability of Sulfolobus solfataricus alcohol dehydrogenase
Extremophiles
13
751-761
2009
Saccharolobus solfataricus
brenda
Machielsen, R.; Looger, L.; Raedts, J.; Dijkhuizen, S.; Hummel, W.; Henneman, H.; Daussmann, T.; van der Oost, J.
Cofactor engineering of Lactobacillus brevis alcohol dehydrogenase by computational design
Eng. Life Sci.
9
38-44
2009
Levilactobacillus brevis
-
brenda
Zhou, S.; Zhang, S.C.; Lai, D.Y.; Zhang, S.L.; Chen, Z.M.
Biocatalytic characterization of a short-chain alcohol dehydrogenase with broad substrate specificity from thermophilic Carboxydothermus hydrogenoformans
Biotechnol. Lett.
35
359-365
2013
Carboxydothermus hydrogenoformans (Q3ACV3), Carboxydothermus hydrogenoformans DSM 6008 (Q3ACV3)
brenda
Protsko, C.; Vieille, C.; Laivenieks, M.; Prasad, L.; Sanders, D.A.; Delbaere, L.T.
Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant
Acta Crystallogr. Sect. F
66
831-833
2010
Thermoanaerobacter ethanolicus (P77990), Thermoanaerobacter ethanolicus
brenda
Li, Y.; Xu, J.; Xu, Y.
Deracemization of aryl secondary alcohols via enantioselective oxidation and stereoselective reduction with tandem whole-cell biocatalysts
J. Mol. Catal. B
64
48-52
2010
Microbacterium oxydans, Microbacterium oxydans ECU2010
-
brenda
Aggarwal, N.; Mandal, P.K.; Gautham, N.; Chadha, A.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330
Acta Crystallogr. Sect. F
69
313-315
2013
Candida parapsilosis (M4VRJ6), Candida parapsilosis ATCC 7330 (M4VRJ6)
brenda
Wang, N.Q.; Sun, J.; Huang, J.; Wang, P.
Cloning, expression, and directed evolution of carbonyl reductase from Leifsonia xyli HS0904 with enhanced catalytic efficiency
Appl. Microbiol. Biotechnol.
98
8591-8601
2014
Leifsonia xyli (T2FLN4), Leifsonia xyli HS0904 (T2FLN4)
brenda
Chen, R.; Liu, X.; Wang, J.; Lin, J.; Wei, D.
Cloning, expression, and characterization of an anti-Prelog stereospecific carbonyl reductase from Gluconobacter oxydans DSM2343
Enzyme Microb. Technol.
70
18-27
2015
Gluconobacter oxydans, Gluconobacter oxydans DSM 2343
brenda
Nealon, C.M.; Welsh, T.P.; Kim, C.S.; Phillips, R.S.
I86A/C295A mutant secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus has broadened substrate specificity for aryl ketones
Arch. Biochem. Biophys.
606
151-156
2016
Thermoanaerobacter ethanolicus
brenda
Peng, Y.; Zhang, Y.
Continuous preparation of (R)-phenyl-ethanediol by catalysis of nanoparticles immobilized bi-enzyme coupling system
Chin. J. Proc. Engin.
16
286-291
2016
Morganella morganii, Morganella morganii CMCC(B)49208
-
brenda
He, S.; Wang, Z.; Zou, Y.; Chen, S.; Xu, X.
Purification and characterization of a novel carbonyl reductase involved in oxidoreduction of aromatic beta-amino ketones/alcohols
Process Biochem.
49
1107-1112
2014
Kocuria rhizophila
-
brenda
Zhou, Y.; Peng, Q.; Zhang, L.; Cheng, S.; Zeng, L.; Dong, F.; Yang, Z.
Characterization of enzymes specifically producing chiral flavor compounds (R)- and (S)-1-phenylethanol from tea (Camellia sinensis) flowers
Food Chem.
280
27-33
2019
Camellia sinensis (A0A494WKI6)
brenda