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IUBMB CommentsThe enzyme, found in bacteria, also acts more slowly on N-acetyl-D-mannosamine.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
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N-acetyl-D-galacotsaminate
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Synonyms
n-acyl-d-hexosamine oxidase,
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N-acyl-beta-D-hexosamine:oxygen 1-oxidoreductase
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N-acyl-D-hexosamine oxidase
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oxidase, N-acyl-D-hexosamine
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RRSL_02030
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N-acetyl-D-galactosamine + O2 + H2O = N-acetyl-D-galacotsaminate + H2O2
(2), overall reaction
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N-acetyl-D-galactosamine + O2 = N-acetyl-D-galactosamino-1,5-lactone + H2O2
(2a)
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N-acetyl-D-galactosamino-1,5-lactone + H2O = N-acetyl-D-galactosaminate
(2b), spontaneous
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N-acetyl-D-glucosamine + O2 + H2O = N-acetyl-D-glucosaminate + H2O2
(1), overall reaction
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N-acetyl-D-glucosamine + O2 = N-acetyl-D-glucosamino-1,5-lactone + H2O2
(1a)
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N-acetyl-D-glucosamino-1,5-lactone + H2O = N-acetyl-D-glucosaminate
(1b), spontaneous
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N-acyl-D-hexosamine:oxygen 1-oxidoreductase
The enzyme, found in bacteria, also acts more slowly on N-acetyl-D-mannosamine.
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chitobiose + O2
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?
D-galactose + O2
?
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?
D-galactose + O2
D-galactono-1,5-lactone + H2O2
D-glucose + O2
D-gluconolactone + H2O2
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0.3% of the activity with N-acetyl-D-glucosamine
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D-mannose + O2
D-mannonolactone + H2O2
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0.38% of the activity with N-acetyl-D-glucosamine
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?
N,N-diacetylchitobiose + O2
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31% of the activity with N-acetyl-D-glucosamine
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N-acetyl-beta-D-glucosamine + O2
N-acetyl-D-glucosaminolactone + H2O2
N-acetyl-D-galactosamine + O2
N-acetyl-D-galactosaminolactone + H2O2
N-acetyl-D-glucosamine + O2
N-acetyl-D-glucosaminate + H2O2
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r
N-acetyl-D-mannosamine + O2
N-acetyl-D-mannosaminolactone + H2O2
N-acetylmuramic acid + O2
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44% of the activity with N-acetyl-D-glucosamine
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N-glycolyl-D-glucosamine + O2
N-glycolyl-D-glucosaminolactone + H2O2
D-galactosamine + O2
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35% of the activity with N-acetyl-D-glucosamine
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D-galactosamine + O2
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D-galactosamine + O2
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D-galactose + O2
D-galactono-1,5-lactone + H2O2
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0.43% of the activity with N-acetyl-D-glucosamine
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D-galactose + O2
D-galactono-1,5-lactone + H2O2
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0.43% of the activity with N-acetyl-D-glucosamine
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D-glucosamine + O2
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14% of the activity with N-acetyl-D-glucosamine
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D-glucosamine + O2
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D-glucosamine + O2
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D-mannosamine + O2
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0.03% of the activity with N-acetyl-D-glucosamine
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D-mannosamine + O2
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N-acetyl-beta-D-glucosamine + O2
N-acetyl-D-glucosaminolactone + H2O2
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alpha-anomer is not oxidized
in presence of H2O: N-acetyl-D-glucosaminate + H2O2
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N-acetyl-beta-D-glucosamine + O2
N-acetyl-D-glucosaminolactone + H2O2
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alpha-anomer is not oxidized
in presence of H2O: N-acetyl-D-glucosaminate + H2O2
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N-acetyl-beta-D-glucosamine + O2
N-acetyl-D-glucosaminolactone + H2O2
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?
N-acetyl-beta-D-glucosamine + O2
N-acetyl-D-glucosaminolactone + H2O2
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N-acetyl-D-galactosamine + O2
N-acetyl-D-galactosaminolactone + H2O2
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98% of the activity with N-acetyl-D-glucosamine
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N-acetyl-D-galactosamine + O2
N-acetyl-D-galactosaminolactone + H2O2
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98% of the activity with N-acetyl-D-glucosamine
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N-acetyl-D-galactosamine + O2
N-acetyl-D-galactosaminolactone + H2O2
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N-acetyl-D-galactosamine + O2
N-acetyl-D-galactosaminolactone + H2O2
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N-acetyl-D-mannosamine + O2
N-acetyl-D-mannosaminolactone + H2O2
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4.9% of the activity with N-acetyl-D-glucosamine
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N-acetyl-D-mannosamine + O2
N-acetyl-D-mannosaminolactone + H2O2
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4.9% of the activity with N-acetyl-D-glucosamine
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N-acetyl-D-mannosamine + O2
N-acetyl-D-mannosaminolactone + H2O2
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N-acetyl-D-mannosamine + O2
N-acetyl-D-mannosaminolactone + H2O2
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N-glycolyl-D-glucosamine + O2
N-glycolyl-D-glucosaminolactone + H2O2
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72% of the activity with N-acetyl-D-glucosamine
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N-glycolyl-D-glucosamine + O2
N-glycolyl-D-glucosaminolactone + H2O2
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72% of the activity with N-acetyl-D-glucosamine
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FAD
absorption spectrum displays maxima at 375 and 445 nm, FAD is bound to residues His64 and Cys123
additional information
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probably contains unidentified flavin component
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SDS
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slightly accelerates activity
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chitobiose
pH 7.4, 25°C
65
D-mannosamine
pH 7.4, 25°C
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N,N-diacetylchitobiose
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N-acetyl muramic acid
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0.26
N-acetyl-beta-D-glucosamine
pH 7.4, 25°C
0.1 - 0.32
N-acetyl-D-galactosamine
0.24
N-acetyl-D-glucosamine
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40 - 182
N-acetyl-D-mannosamine
2.6
N-Glycolyl-D-glucosamine
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1.4
D-galactosamine
pH 7.4, 25°C
102
D-galactose
pH 7.4, 25°C
4.5
D-glucosamine
pH 7.4, 25°C
216
D-glucose
pH 7.4, 25°C
59
D-mannose
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D-mannose
pH 7.4, 25°C
0.1
N-acetyl-D-galactosamine
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0.32
N-acetyl-D-galactosamine
pH 7.4, 25°C
40
N-acetyl-D-mannosamine
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182
N-acetyl-D-mannosamine
pH 7.4, 25°C
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70.4
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N-acetyl-D-glucosamine
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5 - 11
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pH 5.0: about 70% of maximal activity, pH 11: about 50% of maximal activity
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brenda
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brenda
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UniProt
brenda
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UniProt
brenda
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HEXNO_RALSU
Ralstonia solanacearum (strain UW551)
508
0
56364
Swiss-Prot
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22000
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1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
36000
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1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
44000
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1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
63000
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1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
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tetramer
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1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
tetramer
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1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
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3 - 9
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stable at 45°C for 10 min
389762
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melting temperature, in buffers with pH 6.5 and above
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100% remaining activity after 10 min
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50% remaining activity after 10 min
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expression in Escherichia coli
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enzyme is able to refold and regain its activity upon heating to up to 80°C
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analysis
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enzyme may by useful for measurement of N-acetyl-beta-D-glucosaminidase
analysis
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enzyme may by useful for measurement of N-acetyl-beta-D-glucosaminidase
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Horiuchi, T.
Purification and properties of N-acyl-D-hexosamine oxidase from Pseudomonas sp. 15-1
Agric. Biol. Chem.
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361-368
1989
Pseudomonas sp., Pseudomonas sp. 15-1
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brenda
Rembeza, E.; Boverio, A.; Fraaije, M.W.; Engqvist, M.K.M.
Discovery of two novel oxidases using a high-throughput activity screen
Chembiochem
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e202100510
2022
Ralstonia solanacearum (A3RXB7), Ralstonia solanacearum UW551 (A3RXB7)
brenda
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1.1.3.29 N-acylhexosamine oxidase
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