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Information on EC 1.14.12.10 - benzoate 1,2-dioxygenase
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1.14.12.10 benzoate 1,2-dioxygenaseIUBMB Comments
A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), and an iron-sulfur oxygenase. Requires Fe2+.
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Word Map
- 1.14.12.10
- catechol
- calcoaceticus
- toluate
- 2-chlorobenzoate
- chlorocatechols
-
rieske
-
cis-diols
-
rieske-type
-
beta-ketoadipate
-
benzoate-grown
- radioresistens
- 3-methylbenzoate
-
benzoate-induced
-
3,4-dioxygenase
-
cis-dihydrodiols
- 4-chlorocatechol
-
ring-hydroxylating
- arvilla
- synthesis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
benzoate dioxygenase, benzoate 1,2-dioxygenase, benabc, benzoate 1,2-dioxygenase system, benzoate hydroxylase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
BDO
BenA
benABC
benzoate 1,2-dioxygenase
benzoate 1,2-dioxygenase oxygenase
benzoate 1,2-dioxygenase system
benzoate dioxygenase
benzoate hydroxylase
-
-
-
-
benzoic hydroxylase
-
-
-
-
BZDO
BZDO enzyme
EC 1.13.99.2
-
-
formerly
-
oxygenase, benzoate 1,2-di-
-
-
-
-
benABC
-
genes are organized in a single cluster and are under benzoate induced regulation. The benABC genes jointly encode benzoate 1,2-dioxygenase, which catalyzes the dihydroxylation of benzoate to produce 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid
benABC
-
genes are organized in a single cluster and are under benzoate induced regulation. The benABC genes jointly encode benzoate 1,2-dioxygenase, which catalyzes the dihydroxylation of benzoate to produce 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid
-
benzoate 1,2-dioxygenase oxygenase
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
benzoate dioxygenase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
proposed catalytic cycle
-
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
the enzyme consists of two proteins, a NADH-cytochrome c reductase and an oxygenase
-
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
proposed catalytic cycle
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
the enzyme consists of two proteins, a NADH-cytochrome c reductase and an oxygenase
-
-
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
reduction
-
-
-
-
redox reaction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
benzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating)
A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), and an iron-sulfur oxygenase. Requires Fe2+.
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CAS REGISTRY NUMBER
COMMENTARY
9059-18-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
benzene + O2 + NADH
(R,S)-1,2-dihydro-1,2-dihydroxycatechol + NAD+
-
-
-
-
?
benzoate + NADH-cytochrome c reductase component + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+-cytochrome c reductase component
-
-
-
-
r
2-methylbenzoate + O2 + NADH
cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
-
-
-
?
2-methylbenzoate + O2 + NADH
cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
-
-
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
3-aminobenzoate + O2 + NADH
3-amino-2-hydro-1,2-dihydroxybenzoate
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
3-chlorobenzoate + O2 + NADH + H+
3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
benzoate analogues with substituents in meta position are oxygenated more rapidly than those with ortho- or para substitution
-
?
3-fluorobenzoate + O2 + NADH + H+
3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
3-methylbenzoate + O2 + NADH + H+
3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
?
4-fluorobenzoate + O2 + NADH
4-fluoro-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
4-fluorobenzoate + O2 + NADH
4-fluoro-2-hydro-1,2-dihydroxybenzoate
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield).
-
?
benzoate + NADH + H+ + O2
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield).
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
catabolism of aromatic compounds
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
catabolism of aromatic compounds
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
activity in E. coli expressing a presumed dioxygenase component from Rhodococcus
-
-
?
additional information
?
-
-
benzoate induces the expression of benzoate 1,2-dioxygenase
-
-
?
additional information
?
-
-
benzoate 1,2 dioxygenase oxygenase component (BZDO) prepared in a form with the Rieske cluster oxidized and the mononuclear iron in the Fe(III) state can utilize H2O2 as a source of reduced oxygen to form the correct cis-dihydrodiol product from benzoate
-
-
?
additional information
?
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
benzoate 1,2 dioxygenase oxygenase component (BZDO) prepared in a form with the Rieske cluster oxidized and the mononuclear iron in the Fe(III) state can utilize H2O2 as a source of reduced oxygen to form the correct cis-dihydrodiol product from benzoate
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
benzoate induces the expression of benzoate 1,2-dioxygenase
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
-
-
?
benzoate + NADH + H+ + O2
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield).
-
?
benzoate + NADH + H+ + O2
cis-1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylate + NAD+
-
-
Recombinant strain with overexpression of benABC gene and benD knockout. Highest biotransformation is found with cells grown on 2.88 g/l Na-benzoate, yielding approximately 2.3 g/l 1,2-dihydroxy-cyclohexa-3,5-diene-1-carboxylic acid with a yield of 73%. The biotransformation yield decreases along with the increase in substrate benzoate concentration. The benD knockout mutant harboring BZDO genes produced concentration of cis-diol product in fermentation (17 g/l, 61% yield).
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
catabolism of aromatic compounds
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
catabolism of aromatic compounds
-
-
?
benzoate + O2 + NADH
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
benzoate + O2 + NADH + H+
2-hydro-1,2-dihydroxybenzoate + NAD+
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
iron-sulfur center
-
each alpha-subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site
FAD
-
requirement, benzoate 1,2-dioxygenase system consists of 2 proteins: a 37500 Da NADH-cytochrome c reductase containing 1 FAD and 1 iron-sulfur cluster [2Fe-2S] and an oxygenase which may contain 4 [2Fe-2S] clusters
NADH
-
requirement, benzoate 1,2-dioxygenase system consists of 2 proteins: a 37500 Da NADH-cytochrome c reductase containing 1 FAD and 1 iron-sulfur cluster [2Fe-2S] and an oxygenase which may contain 4 [2Fe-2S] clusters
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Iron
Iron
-
reductase component contains a plant type [2Fe-2S] cluster and a presumed mononuclear iron, the terminal dioxygenase contains a Rieske-type [2Fe-2S] cluster
Iron
-
oxygenase component: 2.7 mol/mol of alphabeta subunit, each alpha subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site, reductase component: 2.02 mol/monomer
Iron
-
iron sulfur protein with [2Fe-2S]clusters and additional iron atoms
Iron
-
each alpha-subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
367
benzoate
-
in the presence of saturating amounts of NADH-cytochrome c reductase
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
The recombinant bacteria shows a BZDO activity of 1.2 mmol benzoate per gram cell dry weight, while Pseudomonas putida KTSY01 shows a BZDO activity of only 0.09 mmol.
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Show AA Sequence and Transmembrane Helices (1824 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
19000
-
alpha3,beta3, 3 * 19000 + 3 * 49000, oxygenase component, SDS-PAGE
195000
20000
-
alpha3,beta3, 3 * 20000 + 3 * 50000, oxygenase component of the enzyme system, SDS-PAGE