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Information on EC 1.5.1.22 - Strombine dehydrogenase
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1.5.1.22 Strombine dehydrogenaseIUBMB Comments
Also catalyses the reaction of EC 1.5.1.17 alanopine dehydrogenase, but more slowly. Does not act on L-strombine.
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The enzyme appears in viruses and cellular organisms
Synonyms
strombine dehydrogenase, strombine/alanopine dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Dehydrogenase, strombine
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SDH
StDH
strombine dehydrogenase
strombine/alanopine dehydrogenase
St/AlDH functions physiologically as strombine dehydrogenase
SDH
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StDH
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StDH
shows 68% sequence identity with the tauropine dehydrogenase protein from the marine demosponge Halichondria japonica
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N-(carboxymethyl)-D-alanine + NAD+ + H2O = glycine + pyruvate + NADH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
N-(carboxymethyl)-D-alanine:NAD+ oxidoreductase (glycine-forming)
Also catalyses the reaction of EC 1.5.1.17 alanopine dehydrogenase, but more slowly. Does not act on L-strombine.
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CAS REGISTRY NUMBER
COMMENTARY
79393-84-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Gly + 2-ketobutyrate + NADH + H+
2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O
8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
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?
Gly + glyoxylate + NADH + H+
[(carboxymethyl)amino]propanedioic acid + NAD+ + H2O
3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
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?
Gly + oxaloacetate + NADH + H+
N-carboxymethylglycine + NAD+ + H2O
39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
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?
Gly + pyruvate + NADH + H+
D-strombine + NAD+ + H2O
94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
Gly + pyruvate + NADPH
D-strombine + NADP+ + H2O
less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
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?
glycine + pyruvate + NADH + H+
N-(carboxymethyl)-D-alanine + NAD+ + H2O
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the reduction reaction is preferred
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r
L-Ala + 2-ketobutyrate + NADH + H+
2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O
8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
L-Ala + glyoxylate + NADH + H+
N-(carboxymethyl)-L-Ala + NAD+ + H2O
4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
L-Ala + oxaloacetate + NADH + H+
[[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O
40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
L-Ala + pyruvate + NADH + H+
meso-alanopine + NAD+ + H2O
100% activity
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?
L-Ala + pyruvate + NADPH
meso-alanopine + NADP+ + H2O
less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
L-Cys + pyruvate + NADH + H+
N-(1-carboxyethyl)-L-Cys + NAD+ + H2O
1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
L-Ser + pyruvate + NADH + H+
N-(1-carboxyethyl)-L-Ser + NAD+ + H2O
14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
L-Val + pyruvate + NADH + H+
N-(1-carboxyethyl)-L-Val + NAD+ + H2O
0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
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?
additional information
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the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
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Gly + 2-oxobutanoate + NADH
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at 24.8% of the activity with pyruvate
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Gly + pyruvate + NADH
D-Strombine + NAD+ + H2O
the enzyme is highly specific to glycine. No enzyme activity is observed when other amino acids (alanine and taurine) are used as substrates in the experiment
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?
glycine + pyruvate + NADH
D-strombine + NAD+ + H2O
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Gly and Ala are preferred substrates
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glycine + pyruvate + NADH
D-strombine + NAD+ + H2O
enzyme is specific for glycine
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?
L-2-Aminobutanoate + pyruvate + NADH
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68.3% of the activity with Gly
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L-2-Aminobutanoate + pyruvate + NADH
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at 48% of the activity with Gly
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?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
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101% of the activity with Gly
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?
L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
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at 76% of the activity with Gly
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L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
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94% of the activity with Gly
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L-Ala + pyruvate + NADH
meso-Alanopine + NAD+ + H2O
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in the reverse reaction: 81% of the activity with meso-alanopine compared to strombine
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
glycine + pyruvate + NADH + H+
N-(carboxymethyl)-D-alanine + NAD+ + H2O
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the reduction reaction is preferred
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r
additional information
?
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the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pyruvate
inhibited by an excess of substrate pyruvate; substrate inhibition, in excess, pyruvate enters in competition with the other substrate (glycine and/or NADH) preventing the site of reaction to take all the substrates to proceed with the enzymatic reaction. Its optimal concentration is determined to be round 2.0-2.3 mM
additional information
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the enzyme activity in adductor muscle decreases following the marine-brackish gradient
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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increased activity of alanopine dehydrogenase, ADH, and strombine dehydrogenase, SDH, enzymes in Montipora capitata exposed to prolonged durations of hypoxic conditions
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
9.17
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
9.07
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
11.71
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
12.77
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
40.1
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
39.8
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
382.1
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.85
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.87
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.07
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.07
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
10.2
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
10.2
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.34
pyruvate
determined using constant cosubstrate concentrations (100 mM glycine and 0.3 mM NADH, pH 7.0)
0.96
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
0.97
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4.99
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
50.08
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
51.7
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
23.64
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
5.12
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
21.77
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
23.2
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
3.86
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C