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EC Tree
IUBMB Comments Feruloyl-CoA, cinnamoyl-CoA and sinapoyl-CoA can also act as donors, but more slowly.
The enzyme appears in viruses and cellular organisms
Synonyms
putrescine n-hydroxycinnamoyltransferase, hydroxycinnamoyl-coa:putrescine hydroxycinnamoyltransferase, putrescine hydroxycinnamoyl transferase, hydroxycinnamoyl-coenzyme a:putrescine acyltransferase,
more
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caffeoyl-CoA putrescine N-caffeoyl transferase
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hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase
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hydroxycinnamoyl-coenzyme A:putrescine acyltransferase
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putrescine hydroxycinnamoyl transferase
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putrescine hydroxycinnamoyltransferase
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caffeoyl-CoA + putrescine = CoA + N-caffeoylputrescine
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Acyl group transfer
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caffeoyl-CoA:putrescine N-(3,4-dihydroxycinnamoyl)transferase
Feruloyl-CoA, cinnamoyl-CoA and sinapoyl-CoA can also act as donors, but more slowly.
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4-coumaroyl-CoA + putrescine
CoA + N-coumaroylputrescine
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?
4-fluorocinnamoyl-CoA + putrescine
CoA + N-4-fluorocinnamoylputrescine
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?
caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
caffeoyl-CoA + diaminoheptane
CoA + N-caffeoyl-1,7-diaminoheptane
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low activity
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?
caffeoyl-CoA + diaminohexane
CoA + N-caffeoyl-1,6-diaminohexane
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low activity
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?
caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
cinnamoyl-CoA + putrescine
CoA + N-cinnamoylputrescine
coumaroyl-CoA + putrescine
CoA + N-coumaroylputrescine
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?
feruloyl-CoA + agmatine
CoA + N-feruloylagmatine
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?
feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
additional information
?
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caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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?
caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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cadaverine i.e. 1,5-pentanediamine, low activity
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?
caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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?
caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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?
caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
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?
caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
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?
caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
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?
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
the enzyme is highly specific for putrescine and caffeoyl-CoA
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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?
cinnamoyl-CoA + putrescine
CoA + N-cinnamoylputrescine
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?
cinnamoyl-CoA + putrescine
CoA + N-cinnamoylputrescine
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?
feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
feruloyl-CoA gives 10% of the activity with caffeoyl-CoA
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?
feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
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?
feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
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?
feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
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?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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low activity
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?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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low activity
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?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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low activity
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?
additional information
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substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA
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additional information
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substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA
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additional information
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no activity with spermidine
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additional information
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no activity with monoamines
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additional information
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no activity with monoamines
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additional information
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very poor substrate: caffeoyl-CoA
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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?
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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additional information
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no inhibition by thiol reagents
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0.003 - 0.033
caffeoyl-CoA
0.0025
cinnamoyl-CoA
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with putrescine
0.0125 - 0.0356
feruloyl-CoA
0.1755
agmatine
cosubstrate 4-coumaroyl-CoA, pH 7.5, 30°C
0.322
agmatine
cosubstrate feruloyl-CoA, pH 7.5, 30°C
0.003
caffeoyl-CoA
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pH 10.0
0.024
caffeoyl-CoA
with putrescine, recombinant His-tagged enzyme, pH 7.5, 30°C
0.033
caffeoyl-CoA
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pH 7.8
0.0125
feruloyl-CoA
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with caffeoyl-CoA
0.0194
feruloyl-CoA
cosubstrate agmatine, pH 7.5, 30°C
0.0356
feruloyl-CoA
cosubstrate putrescine, pH 7.5, 30°C
0.01
putrescine
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pH 10.0
0.01
putrescine
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caffeoyl-CoA with 1 mM putrescine
0.05
putrescine
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with 0.25 mM caffeoyl-CoA
0.0661
putrescine
cosubstrate feruloyl-CoA, pH 7.5, 30°C
0.1851
putrescine
cosubstrate 4-coumaroyl-CoA, pH 7.5, 30°C
0.664
putrescine
with caffeoyl-CoA, recombinant His-tagged enzyme, pH 7.5, 30°C
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10
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caffeoyl-CoA, sinapoyl-CoA
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7.2 - 10
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about 50% of activity maximum at pH 7.2 and 10
7.5 - 11
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about 50% of activity maximum at pH 7.5 and 11
7.8 - 10
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at 7.8 about 50% of activity maximum, at 10.0 activity maximum, caffeoyl-CoA + putrescine
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30
assay at
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barley, after 1-4d of infection with Blumeria graminis f. sp. hordei, increased activity
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brenda
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UniProt
brenda
TX1
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brenda
TX4
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brenda
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UniProt
brenda
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brenda
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brenda
TX1
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brenda
TX4
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brenda
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cultured
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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malfunction
deregulatory effects of transcription factor MYB8 silencing on AT1 control, altered metabolite profile, overview. Silencing of acyltransferase AT1 impairs polyamine accumulation
metabolism
the enzyme AT1 is involved in a branch of jasmonic acid signaling that defends Nicotiana attenuata plants against herbivores: jasmonic acid, via key transcription factor MYB8's transcriptional control of AT1 and DH29 genes, controls the entire branch of polyamine biosynthesis, overview. AT1, DH29, and CV86 contribute to distinct, but interconnected, MYB8-controlled hydroxycinnamoylputrescine and hydroxycinnamoylspermidine pools
physiological function
the enzyme is responsible for caffeoylputrescine biosynthesis in tobacco
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G9HTF7_NICAT
438
0
49686
TrEMBL
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PUHT_ORYSJ
452
0
48612
Swiss-Prot
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45000
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HPLC gel filtration
50000
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1 * 50000, SDS-PAGE
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monomer
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1 * 50000, SDS-PAGE
monomer
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1 * 50000, SDS-PAGE
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K267E
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missense mutation identified in cv. Shepody, predicted to change the catalytic activity
P101T/S368N
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missense mutation identified in cv. Shepody, predicted not to change the catalytic activity
T115M
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missense mutation identified in cv. Shepody, predicted to change the catalytic activity
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thawing and refreezing, 20% loss of activity
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rapid denaturation in absence of reducing compounds
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486315
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-20°C, stable for 2 months
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recombinant C-terminally His6-tagged AT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
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AT1 gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged AT1 in Escherichia coli strain BL21(DE3)
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AT1 expression is controlled by the key transcription factor MYB8
expression in leaf is highly induced upon herbivore feeding
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agriculture
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transcript abundances of genes that catalyze the biosynthesis of hydroxycinnamic acid amides, such as 4-coumarate:CoA ligase, tyrosine decarboxylase, ornithine decarboxylase, tyramine hydroxycinnamoyl transferase, and putrescine hydroxycinnamoyl transferase, are higher in the genotypes resistant to late blight induced by Phytophthora infestans
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Cowley, T.; Walters, D.R.
Polyamine metabolism in barley reacting hypersensitively to the powdery mildew fungus Blumeria graminis f. sp. hordei
Plant Cell Environ.
25
461-468
2002
Hordeum vulgare
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brenda
Negrel, J.; Javelle, F.; Paynot, M.
Separation of putrescine and spermidine hydroxycinnamoyl transferases extracted from tobacco callus
Phytochemistry
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1089-1092
1991
Nicotiana tabacum
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brenda
Negrel, J.; Paynot, M.; Javelle, F.
Purification and properties of putrescine hydroxycinnamoyl transferase from tobacco (Nicotiana tabaccum) cell suspensions
Plant Physiol.
98
1264-1269
1992
Nicotiana tabacum, Nicotiana tabacum TX1
brenda
Meurer-Grimes, B.; Berlin, J.; Strack, D.
Hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase in tobacco cell cultures with high and low levels of caffeoylputrescine
Plant Physiol.
89
488-492
1989
Nicotiana tabacum, Nicotiana tabacum TX1, Nicotiana tabacum TX4
brenda
Negrel, J.
The biosynthesis of cinnamoylputrescines in callus tissue cultures of Nicotiana tabaccum
Phytochemistry
28
477-481
1989
Nicotiana tabacum
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brenda
Onkokesung, N.; Gaquerel, E.; Kotkar, H.; Kaur, H.; Baldwin, I.; Galis, I.
MYB8 controls inducible phenolamide levels by activating three novel hydroxycinnamoyl-coenzyme A: polyamine transferases in Nicotiana attenuata
Plant Physiol.
158
389-407
2012
Nicotiana attenuata (G9HTF7), Nicotiana attenuata
brenda
Pushpa, D.; Yogendra, K.; Gunnaiah, R.; Kushalappa, A.; Murphy, A.
Identification of late blight resistance-related metabolites and genes in potato through nontargeted metabolomics
Plant Mol. Biol. Rep.
32
584-595
2014
Solanum tuberosum
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brenda
Tanabe, K.; Hojo, Y.; Shinya, T.; Galis, I.
Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants
J. Integr. Plant Biol.
58
903-913
2016
Oryza sativa Japonica Group (Q7XXN4)
brenda
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