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alphaATP + 5-methylthioribose
alphaADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-butylthioribose
ADP + 5-butylthioribose 1-phosphate
-
-
-
?
ATP + 5-ethylthioribose
ADP + 5-ethylthioribose 1-phosphate
-
-
-
?
ATP + 5-isobutylthioribose
ADP + 5-isobutylthioribose 1-phosphate
ATP + 5-isopropylthioribose
ADP + 5-isopropylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
ATP + 5-propylthioribose
ADP + 5-propylthioribose 1-phosphate
-
-
-
?
ATP + D-ribose
ADP + D-ribose 1-phosphate
-
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + 5-methyl-5-thio-D-ribose 1-phosphate
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
CTP + 5-methylthioribose
CDP + 5-methylthioribose 1-phosphate
-
20% of activity with ATP
-
?
dATP + 5-methylthioribose
dADP + 5-methylthioribose 1-phosphate
-
120% of activity with ATP
-
?
additional information
?
-
ATP + 5-isobutylthioribose
ADP + 5-isobutylthioribose 1-phosphate
-
-
-
?
ATP + 5-isobutylthioribose
ADP + 5-isobutylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
no activity with GTP and UTP
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
no activity with GTP and UTP
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
key step in recycling of methionine from 5'-methylthioadenosine a co-product of polyamine biosynthesis, expression of methylthioribose kinase may be under control of the methionine regulon
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
no activity with AMP, ADP and alpha,beta-CH2-ATP
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
enzyme may be involved in an alternative pathway of methionine synthesis in plant tissues
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + 5-methyl-5-thio-D-ribose 1-phosphate
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + 5-methyl-5-thio-D-ribose 1-phosphate
structure analysis in Arabidopsis thaliana, provides template for structure-based design of agrochemicals, particularly herbicides with the possibility that effectiveness can be regulated by nutrient levels
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
best substrate
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
physiological substrate
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
5-methylthioribose kinase is a key enzyme in methionine salvage in bacteria
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
dissociative mechanism
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle
-
-
?
additional information
?
-
-
the enzyme has significant ribose kinase activity, it has a hydrophobic pocket that specifically recognizes the methylthio group of the substrate. MTK shows a 9fold higher Vmax for ribose kinase activity than for S-methyl-5-thio-D-ribose kinase activity, but also has a several fold higher Km for ribose
-
-
?
additional information
?
-
the enzyme regulation is related to the sulfur metabolism
-
-
?
additional information
?
-
-
the enzyme regulation is related to the sulfur metabolism
-
-
?
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ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
ATP + S-methyl-5-thio-D-ribose
ADP + 5-methyl-5-thio-D-ribose 1-phosphate
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
physiological substrate
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
additional information
?
-
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
key step in recycling of methionine from 5'-methylthioadenosine a co-product of polyamine biosynthesis, expression of methylthioribose kinase may be under control of the methionine regulon
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
enzyme may be involved in an alternative pathway of methionine synthesis in plant tissues
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + 5-methylthioribose
ADP + 5-methylthioribose 1-phosphate
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
-
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
-
5-methylthioribose kinase is a key enzyme in methionine salvage in bacteria
-
-
?
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle
-
-
?
additional information
?
-
the enzyme regulation is related to the sulfur metabolism
-
-
?
additional information
?
-
-
the enzyme regulation is related to the sulfur metabolism
-
-
?
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purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.002 ml of protein solution containing 10 mg/ml protein in 20 mM imidazole, pH 7.6, 2 mM DTT, 1 mM 5-methyl-5-thio-D-ribose and 1 mM ATP-gamma-S, is mixed an equal volume of reservoir solution containing 10% w/v PEG 8000, 0.1 M sodium cacodylate, pH 6.5, and 0.2 M magnesium acetate, equilibration against 0.5 ml reservoir solution, room temperature, 3 days, cryoprotection by soaking in reservoir solution plus 25% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 1.9 A resolution, identification of heavy-atom derivatives
-
structure determination, 1.9 A resolution, diffraction data and crystallographic refinement statistics, similarity to enzyme structure of Bacillus subtilis, conformation of Gly-rich loop more extended in Arabidopsis thaliana than in bacterial enzyme homologues, different conformations of G-loops and W-loops in Arabidopsis thaliana and Bacillus subtilis enzymes despite of high sequence similarities, significant ATPase activity in the absence of 5-methyl-5-thio-D-ribose suggested, features of the substrate binding site offer opportunities for a simple organic salt of a 5-methyl-5-thio-D-ribose analog to specifically inhibit kinase activity
ADP-2Ho and ADP-2Mg complexes
purified recombinant non-tagged enzyme, hanging drop vapour diffusion method, 0.002-0.003 ml of protein solution containing 7.5-15 mg/ml protein in 10 mM potassium phosphate, pH 7.4, 2 mM DTT, 1 mM 5-methyl-5-thio-D-ribose and 1 mM ATP-gamma-S, or 8 mM CHAPS, is mixed an equal volume of reservoir solution containing 22% w/v PEG 2000 monomethylether, 0.1 M Tris-HCl, pH 7.5, and 0.3 M sodium acetate, equilibration against 0.5 ml reservoir solution, room temperature, 3 days, cryoprotection by soaking in reservoir solution plus 25% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 3.0 A resolution without detergent, and at 2.2 A resolution in presence of CHAPS, no crystallization of His-tagged enzyme possible, identification of heavy-atom derivatives
-
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ammonium sulfate, aminohexyl-Sepharose, Sephadex g-200, hydroxyapatite Bio-Gel
-
ammonium sulfate, DEAE-Sepharose, 5-(p-aminophenyl)thioribose affinity chromatography
-
ammonium sulfate, partial purified
-
ammonium sulfate, Sephadex G-200, DEAE-cellulose
-
purified from inclusion bodies, gel filtration
recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off
recombinant His-tagged and non-tagged enzymes from Escherichia coli strain BL21(DE3), the latter by anion exchange chromatography, ultracentrifucation and gel filtration
-
recombinant refolded His-tagged enzyme from Escherichia coli by centrifugation, nickel affinity chromatography, ultrafiltration and gel filtration
-
recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off
recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off
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Kushad, M.M.; Richardson, D.G.; Ferro, A.J.
5'-Methylthioadenosine nucleosidase and 5-methylthioribose kinase activities and ethylene production during tomato fruit development and ripening
Plant Physiol.
79
525-529
1985
Solanum lycopersicum
brenda
Cornell, K.A.; Winter, R.W.; Tower, P.A.; Riscoe, M.K.
Affinity purification of 5-methylthioadenosine kinase and 5-methylthioribose/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae
Biochem. J.
317
285-290
1996
Klebsiella pneumoniae
brenda
Guranowski, A.
Plant 5-methylthioribose kinase
Plant Physiol.
71
932-935
1983
Lupinus luteus
brenda
Ferro, A.J.; Barrett, A.; Shapiro, S.K.
5-Methylthioribose kinase. A new enzyme involved in the formation of methionine from 5-methylthioribose
J. Biol. Chem.
253
6021-6025
1978
Klebsiella aerogenes
brenda
Ferro, A.J.; Marchitto, K.S.
5-Methylthioribose kinase (Enterobacter aerogenes)
Methods Enzymol.
94
361-364
1983
Klebsiella aerogenes
brenda
Tower, P.A.; Alexander, D.B.; Johnson, L.L.; Riscoe, M.K.
Regulation of methylthioribose kinase by methionine in Klebsiella pneumoniae
J. Gen. Microbiol.
139
1027-1031
1993
Klebsiella pneumoniae
brenda
Kushad, M.M.; Richardson, D.G.; Ferro, A.J.
5-Methylthioribose kinase activity in plants
Biochem. Biophys. Res. Commun.
108
167-173
1982
Persea americana, Fragaria x ananassa, Solanum lycopersicum, Malus sp., Pyrus communis
brenda
Sekowska, A.; Mulard, L.; Krogh, S.; Tse, J.K.; Danchin, A.
MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis
BMC Microbiol.
1
15
2001
Bacillus subtilis
brenda
Ku, S.Y.; Yip, P.; Cornell, K.A.; Riscoe, M.K.; Howell, P.L.
Crystallization and preliminary X-ray analysis of 5'-methylthioribose kinase from Bacillus subtilis and Arabidopsis thaliana
Acta Crystallogr. Sect. D
60
116-119
2004
Arabidopsis thaliana, Bacillus subtilis
brenda
Sauter, M.; Cornell, K.A.; Beszteri, S.; Rzewuski, G.
Functional analysis of methylthioribose kinase genes in plants
Plant Physiol.
136
4061-4071
2004
Oryza sativa (Q7XR61), Oryza sativa, Arabidopsis thaliana (Q9C6D2)
brenda
Ku, S.Y.; Smith, G.D.; Howell, P.L.
ADP-2Ho as a phasing tool for nucleotide-containing proteins
Acta Crystallogr. Sect. D
63
493-499
2007
Bacillus subtilis (O31663), Bacillus subtilis
brenda
Ku, S.Y.; Yip, P.; Cornell, K.A.; Behr, J.B.; Guillerm, G.; Howell, P.L.
Structures of 5-methylthioribose kinase reveal substrate specificity and unusual mode of nucleotide binding
J. Biol. Chem.
282
22195-22206
2007
Bacillus subtilis
brenda
Ku, S.Y.; Cornell, K.A.; Howell, P.L.
Structure of Arabidopsis thaliana 5-methylthioribose kinase reveals a more occluded active site than its bacterial homolog
BMC Struct. Biol.
7
70
2007
Arabidopsis thaliana (Q9C6D2), Arabidopsis thaliana
brenda
Pommerrenig, B.; Feussner, K.; Zierer, W.; Rabinovych, V.; Klebl, F.; Feussner, I.; Sauer, N.
Phloem-specific expression of Yang cycle genes and identification of novel Yang cycle enzymes in Plantago and Arabidopsis
Plant Cell
23
1904-1919
2011
Plantago major (Q1EMM5), Arabidopsis thaliana (Q9C6D2)
brenda
Nakano, T.; Saito, Y.; Yokota, A.; Ashida, H.
Plausible novel ribose metabolism catalyzed by enzymes of the methionine salvage pathway in Bacillus subtilis
Biosci. Biotechnol. Biochem.
77
1104-1107
2013
Bacillus subtilis
brenda
Alberton, D.; Mueller-Santos, M.; Brusamarello-Santos, L.C.; Valdameri, G.; Cordeiro, F.A.; Yates, M.G.; de Oliveira Pedrosa, F.; de Souza, E.M.
Comparative proteomics analysis of the rice roots colonized by Herbaspirillum seropedicae strain SmR1 reveals induction of the methionine recycling in the plant host
J. Proteome Res.
12
4757-4768
2013
Oryza sativa (Q7XR61), Oryza sativa
brenda