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(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
(R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O
?
-
amygdalin, low activity
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
100% activity
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
-
sophorose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
-
laminaribiose
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + beta-D-glucose
-
increased activity after acclimation of bacteria to cellobiose medium
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
7.4% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
-
59% activity compared to 2-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
-
activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
beta-D-fucose + 4-nitrophenol
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
130% activity compared to 2-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
beta-D-glucose + 4-nitrophenol
-
20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
4-O-beta-D-galactopyranosyl-D-glucose + H2O
beta-D-galactose + D-glucose
Achatina balteata
-
-
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
6-bromo-2-naphthyl-beta-D-fucoside + H2O
6-bromo-2-naphthol + beta-D-fucose
Achatina balteata
-
-
-
?
6-bromo-2-naphthyl-beta-D-galactoside + H2O
6-bromo-2-naphthol + beta-D-galactose
Achatina balteata
-
-
-
?
6-bromo-2-naphthyl-beta-D-glucoside + H2O
6-bromo-2-naphthol + beta-D-glucose
Achatina balteata
-
-
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
-
gentiobiose
-
?
cellobiose + H2O
2 beta-D-glucose
de-O-acetylated colanic acid + H2O
?
-
substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it
-
-
?
de-pyruvylated colanic acid + H2O
?
-
substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it
-
-
?
lactulose + H2O
D-galactose + D-fructose
laminaribiose + H2O
2 beta-D-glucose
-
-
-
?
o-nitrophenyl-beta-D-galactoside + H2O
o-nitrophenol + beta-D-galactose
Achatina balteata
-
-
-
?
o-nitrophenyl-beta-D-glucoside + H2O
o-nitrophenol + beta-D-glucose
Achatina balteata
-
-
-
?
p-nitrophenyl-2-amino-2-deoxy-D-glucoside + H2O
p-nitrophenol + 2-amino-2-deoxy-D-glucose
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl-alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
p-nitrophenyl-beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
p-nitrophenyl-beta-L-fucoside + H2O
p-nitrophenol + beta-L-fucose
-
-
-
?
sophorose + H2O
alpha-D-glucose + beta-D-glucose
-
-
-
?
additional information
?
-
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
-
-
-
?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
-
salicin, low activity
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
the enzyme also shows beta-fucosidase activity
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
the enzyme also shows beta-fucosidase activity
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
about 60% of the activity with 4-nitrophenyl beta-D-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
about 60% of the activity with 4-nitrophenyl beta-D-fucopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
best substrate
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
best substrate
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
relative activity of 125% as compared to 4-nitrophenyl beta-D-glucopyranoside. Maximum hydrolytic activity for beta-D-fucopyranoside followed by beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
133% activity compared to 2-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
75% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
-
recombinant mutant H540V/N604T of beta-galactosidase, EC 3.2.1.22
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
43% relative to 2-nitrophenyl-beta-D-galactoside hydrolysis
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
43% relative to 2-nitrophenyl-beta-D-galactoside hydrolysis
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
beta-D-fucose + 4-nitrophenol
-
strictly specific
-
?
4-nitrophenyl beta-D-fucoside + H2O
beta-D-fucose + 4-nitrophenol
-
80% activity compared to beta-D-glucoside
-
?
4-nitrophenyl beta-D-fucoside + H2O
beta-D-fucose + 4-nitrophenol
-
implication in cell wall degradation
the only products released from hypokotyl cell walls
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
about 30% of the activity with 4-nitrophenyl beta-D-fucopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
about 30% of the activity with 4-nitrophenyl beta-D-fucopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
maximum hydrolytic activity for beta-D-fucopyranoside followed by beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
82% activity compared to 2-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
maximum hydrolytic activity for beta-D-fucopyranoside followed by beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
71% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
4% activity compared to 2-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
11% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
Achatina balteata
-
-
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
-
cellobiose, weak activity after prolonged incubation
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
-
cellobiose, weak activity after prolonged incubation
-
?
cellobiose + H2O
2 beta-D-glucose
-
-
-
?
cellobiose + H2O
2 beta-D-glucose
-
-
-
?
colanic acid + H2O
?
-
degradation
-
-
?
colanic acid + H2O
?
-
substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it
-
-
?
lactulose + H2O
D-galactose + D-fructose
the enzyme also shows beta-fucosidase activity
-
-
?
lactulose + H2O
D-galactose + D-fructose
the enzyme also shows beta-fucosidase activity
-
-
?
p-nitrophenyl-alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
-
low activity
-
?
p-nitrophenyl-alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
-
low activity
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
Achatina balteata
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
Achatina balteata
-
specific for the beta-anomeric configuration of the glycosidic linkage, high binding specificity towards beta-D-fucoside for both beta-D-fucosidases I + II
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
Achatina balteata
-
maximum catalytic activity
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
Achatina balteata
-
maximum catalytic activity
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
high activity
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
80% activity compared to the activity towards beta-D-glucoside
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
high activity
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
strict beta-D-fucosidase, no stereospecificity
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
common active site for beta-D-fucosidase and beta-D-glucosidase, transglycosylation activity, independent active site for beta-D-galactosidase
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
common active site for beta-D-fucosidase and beta-D-glucosidase, transglycosylation activity, independent active site for beta-D-galactosidase
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
highest activity towards beta-D-fucoside, also beta-D-galactosidase, beta-D-glucosidase and alpha-L-arabinosidase activities
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
Achatina balteata
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
Achatina balteata
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
low activity
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
worst substrate
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
low activity
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
low activity
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
Achatina balteata
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
Achatina balteata
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
best substrate
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
high activity
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-glucoside and p-nitrophenyl-beta-D-fucoside
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-glucoside and p-nitrophenyl-beta-D-fucoside
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
-
-
?
p-nitrophenyl-beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
Achatina balteata
-
-
-
?
p-nitrophenyl-beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
low activity
-
?
p-nitrophenyl-beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
low activity
-
?
additional information
?
-
no beta-glucosidase, beta-arabinosidase or beta-xylosidase activity can be detected
-
-
?
additional information
?
-
-
no beta-glucosidase, beta-arabinosidase or beta-xylosidase activity can be detected
-
-
?
additional information
?
-
no beta-glucosidase, beta-arabinosidase or beta-xylosidase activity can be detected
-
-
?
additional information
?
-
-
no substrate: xylan polysaccharides
-
-
?
additional information
?
-
-
no substrate: xylan polysaccharides
-
-
?
additional information
?
-
-
revealed the highest relative enzymatic activity against cellobiose (100%; beta-1->4 bond), compared with its relative activities against lactose (47%; beta-1->4 bond), sophorose (44%, beta-1->2 bond), and gentiobiose (7%, beta1->6 bond)
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl beta-D-galacturonide, 4-nitrophenyl beta-L-arabinopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-glucuronide, and 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
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1-amino-1-deoxy-D-glucose
-
-
1-butanol
-
inhibition above 50 mM
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
-
1-propanol
-
inhibition above 50 mM
2-amino-2-deoxy-D-glucose
-
non-competitive inhibition
4-O-alpha-D-glucopyranosyl-D-glucose
-
effective inhibitor at 50 mM
4-O-beta-D-galactopyranosyl-D-glucose
4-O-beta-D-glucopyranosyl-D-glucose
acetone
-
inhibition at low concentrations
acetonitrile
-
inhibition at low concentrations
Ba2+
-
strong inhibition at 25 mM
beta-D-fructofuranosyl-alpha-D-glucopyranoside
-
-
Ca2+
Achatina balteata
-
slight inhibition at 50 mM, beta-D-fucosidase II not affected
Co2+
Achatina balteata
-
50% inhibition at 50 mM for beta-D-fucosidase I, 22% inhibition for beta-D-fucosidase II
colanic acid
-
substrate inhibition at high concentrations
conduritol B epoxide
-
loss of activity towards both substrates beta-D-glucoside and beta-D-fucoside, inactivation followed an all-or-none-type of modification, inactivation reduced in the presence of Tris
CuSO4
-
64% inhibition at 1 mM, alpha-L-arabinosidase activity less affected
D-fucono-1,4-lactone
-
50% inhibition at 0.057 mM
dicarbonic acid diethyl ester
-
-
ethanol
-
inhibition above 50 mM
F-
Achatina balteata
-
slight inhibition at 50 mM, beta-D-fucosidase II less affected
Fe3+
-
complete inhibition at 25 mM
glycerol
interferes with enzyme activity
iodoacetate
-
strong inhibition
KI
Achatina balteata
-
lactose protects against inactivation
La3+
-
strong inhibition at 25 mM
methanol
-
inhibition above 50 mM
Mn2+
Achatina balteata
-
slight inhibition at 50 mM, beta-D-fucosidase II less affected
N-Acetylimidazole
Achatina balteata
-
progressive inactivation
N-ethylmaleimide
-
strong inhibition
Ni2+
Achatina balteata
-
47% inhibition at 50 mM for beta-D-.fucosidase I, 25% inhibition at 50 mM for beta-D-fucosidase II
p-nitrophenyl-beta-D-fucoside
-
competitive inhibition of beta-D-glucosidase
p-nitrophenyl-beta-D-glucoside
-
competitive inhibition of beta-D-fucosidase
Tetranitromethane
Achatina balteata
-
lactose protects against inactivation
4-O-beta-D-galactopyranosyl-D-glucose
Achatina balteata
-
competitive inhibition
4-O-beta-D-galactopyranosyl-D-glucose
-
-
4-O-beta-D-glucopyranosyl-D-glucose
-
cellobiose
4-O-beta-D-glucopyranosyl-D-glucose
-
cellobiose
Ag+
-
strong inhibition
beta-D-fucose
-
competitive inhibitor
beta-D-fucose
-
inhibition of beta-D-glucosidase activity, beta-D-fucosidase activity hardly affected
beta-D-fucose
-
strong competitive inhibition
beta-D-fucose
-
competitive inhibitor
beta-D-fucose
-
competitive inhibitor
beta-D-fucose
-
strong competitive inhibition
beta-D-fucose
-
24.5% inhibition at 20 mM for beta-D-galactosidase activity, 8.25% inhibition for beta-D-fucosidase activity
beta-D-galactose
-
non-competitive inhibition
beta-D-galactose
-
competitive inhibition
beta-D-galactose
-
non-competitive inhibition
beta-D-galactose
-
competitive inhibition
beta-D-galactose
-
7.5% inhibition at 20 mM for beta-D-galactosidase activity, 6.5% inhibition at 20 mM for beta-D-fucosidase activity
beta-D-glucose
-
non-competitive inhibitor
beta-D-glucose
-
inhibition of beta-D-glucosidase activity, not beta-D-fucosidase activity
beta-D-glucose
-
competitive inhibition
beta-D-glucose
-
weak competitive inhibition
Cu2+
Achatina balteata
-
complete inhibition for both beta-D-fucosidases I + II at 50 mM
Cu2+
-
strong inhibition at 25 mM
Cu2+
-
inhibition at 1 mM
D-fucono-gamma-lactone
-
-
D-fucono-gamma-lactone
-
effective inhibition at 50 mM
D-galactono-1,4-lactone
-
50% inhibition at 11 mM, competitive inhibition
D-galactono-1,4-lactone
-
64% inhibition at 20 mM for beta-D-galactosidase activity, 29.5% inhibition at 20 mM for beta-D-fucosidase activity
EDTA
-
inhibition at 25 mM
glucono 1,5-lactone
-
inhibitory between 0.1 and 1 mM
glucono 1,5-lactone
-
effective inhibition at 50 mM
Hg2+
-
complete inhibition at 25 mM
Hg2+
-
inhibition at 1 mM
Hg2+
-
strong inhibition at 0.001 mM
Hg2+
-
effective inhibitor at 10 mM
Hg2+
-
89% inhibition at 1 mM
KCN
-
effective inhibitor at 10 mM
KCN
-
70% inhibition at 1 mM, alpha-L-arabinosidase activity less affected
p-chloromercuribenzoate
-
strong inhibition
p-chloromercuribenzoate
-
inhibition at 0.01 mM
p-chloromercuribenzoate
-
strong inhibition between 0.01 mM and 0.001 mM
Tris
Achatina balteata
-
competitive inhibitor; non-competitive inhibition for beta-D-fucosidase I, competitive inhibition for beta-D-fucosidase II
Tris
-
competitive inhibitor
Tris
-
effective inhibitor at 10 mM
Urea
Achatina balteata
-
activity of fucosidase I more rapidly lost than that of fucosidase II in 6 M urea
Urea
-
lowers beta-D-glucosidase and beta-D-fucosidase activities, not beta-D-galactosidase activity
Zn2+
Achatina balteata
-
strong inhibition at 50 mM, beat-D-fucosidase II less affected
Zn2+
-
strong inhibition at 25 mM
additional information
loss in activity if dithiothreitol or 2-mercaptoethanol is omitted from buffers although this effect appears to be reversible, indicating the presence of an active thiol in the enzyme
-
additional information
-
loss in activity if dithiothreitol or 2-mercaptoethanol is omitted from buffers although this effect appears to be reversible, indicating the presence of an active thiol in the enzyme
-
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0.87
2-nitrophenyl beta-D-galactoside
pH 7.2, 22°C, the enzyme also shows beta-fucosidase activity
0.46 - 3.93
4-nitrophenyl beta-D-fucopyranoside
0.64 - 13.3
4-nitrophenyl beta-D-galactopyranoside
0.49 - 9.23
4-nitrophenyl beta-D-glucopyranoside
0.4 - 1.08
4-nitrophenyl beta-D-mannopyranoside
2.56
4-nitrophenyl-beta-D-fucopyranoside
-
25°C, recombinant mutant H540V/N604T of beta-galactosidase, EC 3.2.1.22
0.26 - 8
p-nitrophenyl-beta-D-fucoside
0.25 - 3.5
p-nitrophenyl-beta-D-galactoside
0.1 - 5.37
p-nitrophenyl-beta-D-glucoside
additional information
additional information
-
0.46
4-nitrophenyl beta-D-fucopyranoside
pH 4.6, 37°C, native enzyme
0.47
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, without additive
0.51
4-nitrophenyl beta-D-fucopyranoside
pH 4.6, 37°C, recombinant enzyme
0.65
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
0.94
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, wild-type
0.98
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
1.29
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
1.3
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
3.93
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
0.64
4-nitrophenyl beta-D-galactopyranoside
pH 4.6, 37°C, native enzyme
0.68
4-nitrophenyl beta-D-galactopyranoside
pH 4.6, 37°C, recombinant enzyme
6.18
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
7.44
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
7.65
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, without additive
8.21
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
13.3
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
0.49
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, without additive
0.53
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 37°C, native enzyme
0.58
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 37°C, recombinant enzyme
0.77
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
0.83
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
2.22
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
2.43
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
4.6
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, wild-type
9.23
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
0.4
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
1.08
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, wild-type
0.81
cellobiose
pH 4.6, 37°C, native enzyme
0.87
cellobiose
pH 4.6, 37°C, recombinant enzyme
0.26
p-nitrophenyl-beta-D-fucoside
-
-
0.29
p-nitrophenyl-beta-D-fucoside
-
-
0.33
p-nitrophenyl-beta-D-fucoside
-
-
0.54
p-nitrophenyl-beta-D-fucoside
-
-
0.57
p-nitrophenyl-beta-D-fucoside
-
in presence of inhibitory Tris
0.63
p-nitrophenyl-beta-D-fucoside
-
-
0.7
p-nitrophenyl-beta-D-fucoside
-
-
2.4
p-nitrophenyl-beta-D-fucoside
-
-
6.58
p-nitrophenyl-beta-D-fucoside
-
-
8
p-nitrophenyl-beta-D-fucoside
-
-
0.25
p-nitrophenyl-beta-D-galactoside
-
-
0.3
p-nitrophenyl-beta-D-galactoside
-
-
2.22
p-nitrophenyl-beta-D-galactoside
-
-
3.5
p-nitrophenyl-beta-D-galactoside
-
-
0.1
p-nitrophenyl-beta-D-glucoside
-
-
0.2
p-nitrophenyl-beta-D-glucoside
-
-
0.39
p-nitrophenyl-beta-D-glucoside
-
-
1.3
p-nitrophenyl-beta-D-glucoside
-
-
4.7
p-nitrophenyl-beta-D-glucoside
-
in presence of inhibitory Tris
5.37
p-nitrophenyl-beta-D-glucoside
-
-
additional information
additional information
-
kinetics
-
additional information
p-nitro-phenyl-beta-D-fucoside
-
0.37 mg/ml
additional information
p-nitro-phenyl-beta-D-fucoside
-
0.3 mg/ml
additional information
p-nitro-phenyl-beta-D-fucoside
-
0.39 mg/ml
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14.9 - 463
4-nitrophenyl beta-D-fucopyranoside
8.57 - 24
4-nitrophenyl beta-D-galactopyranoside
17.9 - 129
4-nitrophenyl beta-D-glucopyranoside
47.21 - 51.72
4-nitrophenyl beta-D-mannopyranoside
14.9
4-nitrophenyl beta-D-fucopyranoside
pH 4.6, 37°C, recombinant enzyme
32.1
4-nitrophenyl beta-D-fucopyranoside
pH 4.6, 37°C, native enzyme
74.5
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, without additive
124
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
144
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
181
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
250.7
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
342.81
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, wild-type
463
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
8.57
4-nitrophenyl beta-D-galactopyranoside
pH 4.6, 37°C, recombinant enzyme
11.5
4-nitrophenyl beta-D-galactopyranoside
pH 4.6, 37°C, native enzyme
12.5
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, without additive
14
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
17.3
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
20.7
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
24
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
17.9
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 37°C, recombinant enzyme
22.6
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 37°C, native enzyme
36.9
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
49.48
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, wild-type
63.7
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, without additive
96.8
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
118
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
129
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
129
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
47.21
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, wild-type
51.72
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
7.99
cellobiose
pH 4.6, 37°C, recombinant enzyme
10.1
cellobiose
pH 4.6, 37°C, native enzyme
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Zeng, Y.; Li, Y.; Gu, Y.; Zhang, S.
Purification and characterization of a strictly specific beta-D-fucosidase from Aspergillus phoenicis
Arch. Biochem. Biophys.
1
226-230
1992
Aspergillus phoenicis
brenda
Srisomsap, C.; Svasti, J.; Surarit, R.; Champattanachai, V.; Sawangareetrakul, P.; Boonpuan, K.; Subhasitanont, P.; Chokchaichamnankit, D.
Isolation and characterization of an enzyme with beta-glucosidase and beta-fucosidase activities from Dalbergia cochinchinensis Pierre
J. Biochem.
119
585-590
1996
Dalbergia cochinchinensis
brenda
Surarit, R.; Matsui, H.; Chiba, S.; Svasti, J.; Srisomsap, C.
Chemical modification of beta-glucosidase/beta-fucosidase from Dalbergia cochinchinensis Pierre by conduritol B epoxide
Biosci. Biotechnol. Biochem.
60
1265-1268
1996
Dalbergia cochinchinensis
-
brenda
Surarit, R.; Matsui, H.; Chiba, S.; Svasti, J.; Srisomsap, C.
Evidence for a single active site in beta-D-glucosidase/beta-D-fucosidase from Dalbergia cochinchinensis seeds
Biosci. Biotechnol. Biochem.
61
93-95
1997
Dalbergia cochinchinensis
-
brenda
Nunoura, N.; Ohdan, K.; Yano, T.; Yamamoto, K.; Kumagai, H.
Purification and characterization of beta-D-glucosidase (beta-D-fucosidase) from Bifidobacterium berve clb acclimated to cellobiose
Biosci. Biotechnol. Biochem.
60
188-193
1996
Bifidobacterium breve clb
brenda
Giordani, R.; Lafon, L.
A beta-D-fucosidase from Asclepias curassavica latex
Phytochemistry
33
1327-1331
1993
Asclepias curassavica
-
brenda
Giordani, R.; Noat, G.
Isolation, molecular properties and kinetic studies of a strict beta-fucosidase from Lactuca sativa latex
Eur. J. Biochem.
175
619-625
1988
Lactuca sativa
brenda
Hebraud, M.; Fevre, M.
Characterization of glycoside and polysaccharide hydrolases secreted by the rumen anaerobic fungi Neocallimastix frontalis, Sphaeromonas communis and Piromonas communis
J. Gen. Microbiol.
134
1123-1129
1988
Neocallimastix frontalis, Piromyces communis, Caecomyces communis
-
brenda
Melgar, M.J.; Cabezas, J.A.; Calvo, P.
Kinetic studies on beta-D-fucosidase of Littorina littorea L.
Comp. Biochem. Physiol. B
80
149-156
1985
Littorina littorea
-
brenda
Chinchetru, M.A.; Cabezas, J.A.; Calvo, P.
Characterization and kinetics of beta-D-gluco/fuco/galactosidase from sheep liver
Comp. Biochem. Physiol. B
75
719-728
1983
Ovis aries aries
brenda
Rodriguez, J.A.; Cabezas, J.A.; Calvo, P.
beta-Fucosidase, beta-glucosidase and beta-galactosidase activities associated in bovine liver
Int. J. Biochem.
14
695-698
1982
Bos taurus
brenda
Colas, B.
Inactivation of beta-fucosidase by iodine, N-acetylimidazole and tetranitromethane. Evidence for the existence of essential tyrosine residues
Biochim. Biophys. Acta
657
535-538
1981
Achatina balteata
brenda
Colas, B.
Kinetic studies on beta-D-fucosidases of Achatina balteata
Biochim. Biophys. Acta
613
448-458
1980
Achatina balteata
brenda
Colas, B.
Some physicochemical and structural properties of two beta-fucosidases from Achatina balteata
Biochim. Biophys. Acta
527
150-158
1978
Achatina balteata
brenda
Yamada, M.; Ikeda, K.; Egami, F.
Further studies on glycosidases from liver of Turbo cornutus with special reference to beta-galactosidase, alpha-L-arabinosidase and beta-D-fucosidase activities
J. Biochem.
73
69-76
1973
Turbo cornutus
brenda
Wiederschain, G.Y.; Prokopenkov, A.A.
beta-D-Galactosidase and beta-D-fucosidase of pig kidney
Arch. Biochem. Biophys.
158
539-543
1971
Sus scrofa
brenda
Levvy, G.A.; McAllan, A.
beta-D-Fucosidase in the limpet, Patella vulgata
Biochem. J.
87
206-209
1963
Patella vulgata
brenda
Verhoef, R.; Beldman, G.; Schols, H.A.; Siika-aho, M.; Raettoe, M.; Buchert, J.; Voragen, A.G.
Characterisation of a 1,4-beta-fucoside hydrolase degrading colanic acid
Carbohydr. Res.
340
1780-1788
2005
Citrobacter sp.
brenda
Parikh, M.R.; Matsumura, I.
Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase
J. Mol. Biol.
352
621-628
2005
Escherichia coli
brenda
Jacobson, R.L.; Studentsky, L.; Schlein, Y.
Glycolytic and chitinolytic activities of Phlebotomus papatasi (Diptera: Psychodidae) from diverse ecological habitats
Folia Parasitol.
54
301-309
2007
Phlebotomus papatasi
brenda
Yoshida, S.; Park, D.S.; Bae, B.; Mackie, R.; Cann, I.K.; Nair, S.K.
Structural and functional analyses of a glycoside hydrolase family 5 enzyme with an unexpected beta-fucosidase activity
Biochemistry
50
3369-3375
2011
Prevotella bryantii, Prevotella bryantii B14
brenda
Holmes, M.L.; Scopes, R.K.; Moritz, R.L.; Simpson, R.J.; Englert, C.; Pfeifer, F.; Dyall-Smith, M.L.
Purification and analysis of an extremely halophilic beta-galactosidase from Haloferax alicantei
Biochim. Biophys. Acta
1337
276-286
1997
Haloferax lucentense (P94804), Haloferax lucentense, Haloferax lucentense DSM 14919 (P94804)
brenda
Suzuki, H.; Okazaki, F.; Kondo, A.; Yoshida, K.
Genome mining and motif modifications of glycoside hydrolase family 1 members encoded by Geobacillus kaustophilus HTA426 provide thermostable 6-phospho-beta-glycosidase and beta-fucosidase
Appl. Microbiol. Biotechnol.
97
2929-2938
2013
Geobacillus kaustophilus
brenda
Wierzbicka-Wos, A.; Bartasun, P.; Cieslinski, H.; Kur, J.
Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with beta-glucosidase, beta-fucosidase and beta-galactosidase activities
BMC Biotechnol.
13
22
2013
Shewanella sp.
brenda
Matsuzawa, T.; Yaoi, K.
Screening, identification, and characterization of a novel saccharide-stimulated beta-glycosidase from a soil metagenomic library
Appl. Microbiol. Biotechnol.
101
633-646
2017
uncultured microorganism (A0A1E1FFN6)
brenda
Hwa, K.Y.; Subramani, B.; Shen, S.T.; Lee, Y.M.
Exchange of active site residues alters substrate specificity in extremely thermostable beta-glycosidase from Thermococcus kodakarensis KOD1
Enzyme Microb. Technol.
77
14-20
2015
Thermococcus kodakarensis (Q9YGB8)
brenda
Kar, B.; Verma, P.; Patel, G.K.; Sharma, A.K.
Molecular cloning, characterization and in silico analysis of a thermostable beta-glucosidase enzyme from Putranjiva roxburghii with a significant activity for cellobiose
Phytochemistry
140
151-165
2017
Putranjiva roxburghii (A0A023MIF8), Putranjiva roxburghii
brenda