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5-fluorocytidine + H2O
D-ribose + 5-fluorocytosine
-
-
-
?
5-fluorouridine + H2O
D-ribose + 5-fluorouracil
-
-
-
?
5-methyluridine + H2O
5-methyluracil + D-ribose
-
-
-
-
?
5-methyluridine + H2O
D-ribose + 5-methyluracil
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
cytidine + H2O
cytosine + D-ribose
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
deoxycytidine + H2O
D-deoxyribose + cytosine
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
isopentenyladenine riboside + H2O
D-ribose + N6-isopentenyladenine
-
-
-
-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
nicotinic acid riboside + H2O
nicotinic acid + D-ribose
nicotinic acid riboside methyl ester + H2O
?
ribothymidine + H2O
D-ribose + ?
-
-
-
-
?
uridine + H2O
D-ribose + uracil
uridine + H2O
uracil + D-ribose
xanthosine + H2O
D-ribose + xanthine
additional information
?
-
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
15% of the activity with uridine
-
-
?
adenosine + H2O
D-ribose + adenine
3.5% of the activity with uridine
-
-
?
cytidine + H2O
D-ribose + cytosine
-
-
-
?
cytidine + H2O
D-ribose + cytosine
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
-
3.9% of the activity with uridine
-
-
?
cytidine + H2O
D-ribose + cytosine
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
-
-
-
?
cytidine + H2O
D-ribose + cytosine
5.0% of the activity with uridine
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
22% of the activity with uridine
-
-
?
inosine + H2O
D-ribose + hypoxanthine
9.3% of the activity with uridine
-
-
?
inosine + H2O
D-ribose + hypoxanthine
9.5% of the activity with uridine
-
-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
-
the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase
-
-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
-
the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase
-
-
?
nicotinic acid riboside + H2O
nicotinic acid + D-ribose
-
-
-
-
?
nicotinic acid riboside + H2O
nicotinic acid + D-ribose
-
-
-
-
?
nicotinic acid riboside methyl ester + H2O
?
-
-
-
-
?
nicotinic acid riboside methyl ester + H2O
?
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
?
uridine + H2O
D-ribose + uracil
-
isoform NSH1 shows highest hydrolysis capacity for uridine
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
?
uridine + H2O
D-ribose + uracil
-
best substrate
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
best substrate
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
?
uridine + H2O
D-ribose + uracil
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
?
uridine + H2O
D-ribose + uracil
best substrate
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
-
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
-
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
61% of the activity with uridine
-
-
?
xanthosine + H2O
D-ribose + xanthine
34% of the activity with uridine
-
-
?
xanthosine + H2O
D-ribose + xanthine
53% of the activity with uridine
-
-
?
additional information
?
-
the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
-
-
?
additional information
?
-
-
substrate specificity, overview. No activity with 2'-OH and 3'-OH deoxynucleosides. Nucleosides in which additional groups are added to the exocyclic N6 amino group also exhibit no activity. Nucleosides lacking the 5'-OH group or with the 2'-OH group in the arabino configuration exhibit greatly reduced activity. Purine nucleosides and pyrimidine nucleosides in which the N7 or N3 nitrogens respectively are replaced with carbon also have no activity
-
-
?
additional information
?
-
-
adenosine is a poor substrates, no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
-
-
?
additional information
?
-
-
the enzyme is involved in the metabolism of pyridine nucleotides
-
-
?
additional information
?
-
-
the enzyme is involved in cytidine metabolism
-
-
?
additional information
?
-
-
key enzyme of the pyrimidine salvage pathway, overview, enzyme is required for growth of mutants deficient in uridine kinase activity
-
-
?
additional information
?
-
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
-
-
?
additional information
?
-
-
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
-
-
?
additional information
?
-
-
inosine, adenosine, guanosine, and thymidine are poor substrates
-
-
?
additional information
?
-
substrate specificity, enzyme possesses no phosphorylase activity
-
-
?
additional information
?
-
-
substrate specificity, enzyme possesses no phosphorylase activity
-
-
?
additional information
?
-
-
no or almost no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
-
-
?
additional information
?
-
no or almost no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
-
-
?
additional information
?
-
-
poor activity with cytidine, guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
-
-
?
additional information
?
-
poor activity with cytidine, guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview
-
-
?
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0.44 - 1.66
5-methyluridine
0.682
cytidine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
0.016
nicotinamide riboside
-
in 50 mM Tris-HCl, pH 7.0
0.15
nicotinic acid riboside
-
in 50 mM Tris-HCl, pH 7.0
0.045
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
0.421
ribothymidine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
additional information
additional information
-
0.44
5-methyluridine
-
at 30°C, pH not specified in the publication
0.06
adenosine
pH 7.5, 30°C, recombinant enzyme
0.111
adenosine
pH 7.5, 30°C, recombinant enzyme
0.416
adenosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
0.7
adenosine
-
at 30°C, pH not specified in the publication
0.422
Inosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
0.713
Inosine
pH 7.5, 30°C, recombinant enzyme
1.013
Inosine
pH 7.5, 30°C, recombinant enzyme
1.4
Inosine
-
at 30°C, pH not specified in the publication
0.1
uridine
-
-
0.408
uridine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
0.468
uridine
pH 7.5, 30°C, recombinant enzyme
0.512
uridine
pH 7.5, 30°C, recombinant enzyme
0.8
uridine
recombinant enzyme expressed in Escherichia coli
0.8
uridine
-
at 30°C, pH not specified in the publication
1.6
uridine
-
in 50 mM Tris-HCl, pH 7.0
2.1
uridine
recombinant enzyme expressed in Saccharomyces cerevisiae
0.109
Xanthosine
pH 7.5, 30°C, recombinant enzyme
0.178
Xanthosine
pH 7.5, 30°C, recombinant enzyme
0.454
Xanthosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
1.69
Xanthosine
-
at 30°C, pH not specified in the publication
additional information
additional information
kinetics
-
additional information
additional information
-
substrate specificity and kinetics, overview
-
additional information
additional information
substrate specificity and kinetics, overview
-
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1.12
cytidine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
23.4
nicotinamide riboside
-
in 50 mM Tris-HCl, pH 7.0
1.8
nicotinic acid riboside
-
in 50 mM Tris-HCl, pH 7.0
2.3
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
1.29
ribothymidine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
0.18
adenosine
pH 7.5, 30°C, recombinant enzyme
0.4
adenosine
pH 7.5, 30°C, recombinant enzyme
1.15
adenosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
0.61
Inosine
pH 7.5, 30°C, recombinant enzyme
4.1
Inosine
pH 7.5, 30°C, recombinant enzyme
4.31
Inosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
3
uridine
pH 7.5, 30°C, recombinant enzyme
10.85
uridine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
20
uridine
-
in 50 mM Tris-HCl, pH 7.0
23.8
uridine
pH 7.5, 30°C, recombinant enzyme
1.3
Xanthosine
pH 7.5, 30°C, recombinant enzyme
4.6
Xanthosine
pH 7.5, 30°C, recombinant enzyme
6.3
Xanthosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
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16
cytidine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
31
ribothymidine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
2.8
adenosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
3
adenosine
pH 7.5, 30°C, recombinant enzyme
3.5
adenosine
pH 7.5, 30°C, recombinant enzyme
0.6
Inosine
pH 7.5, 30°C, recombinant enzyme
5.7
Inosine
pH 7.5, 30°C, recombinant enzyme
10.2
Inosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
6.4
uridine
pH 7.5, 30°C, recombinant enzyme
26
uridine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
46
uridine
pH 7.5, 30°C, recombinant enzyme
7.5
Xanthosine
pH 7.5, 30°C, recombinant enzyme
13
Xanthosine
-
pH 7.2, 32°C, recombinant His-tagged enzyme
42
Xanthosine
pH 7.5, 30°C, recombinant enzyme
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Murray, M.G.; Ross, C.
Molecular weight estimations of some pyrimidine-metabolizing enzymes from pea cotyledons by gel filtration
Phytochemistry
10
2645-2648
1971
Pisum sativum
-
brenda
Lee, W.J.
Uridine nucleosidase from malt
J. Am. Soc. Brew. Chem.
45
131-135
1987
Hordeum vulgare
-
brenda
Magni, G.
Uridine nucleosidase from yeast
Methods Enzymol.
51
290-296
1978
Saccharomyces cerevisiae
brenda
Magni, G.; Natalini, P.; Ruggieri, S.; Vita, A.
Baker's yeast uridine nucleosidase is a regulatory copper containing protein
Biochem. Biophys. Res. Commun.
69
724-730
1976
Saccharomyces cerevisiae
brenda
Vita, A.; Natalini, P.; Ipata, P.L.; Magni, G.
Baker's yeast uridine nucleosidase. II. Enzymatic and kinetic properties
Boll. Soc. Ital. Biol. Sper.
50
1077-1083
1974
Saccharomyces cerevisiae
brenda
Corradetti, E.; Natalini, P.; Ipata, P.L.; Magni, G.
Baker's yeast uridine nucleosidase. I. Purification to homogeneity
Boll. Soc. Ital. Biol. Sper.
50
1070-1076
1974
Saccharomyces cerevisiae
brenda
Magni, G.; Fioretti, E.; Ipata, P.L.; Natalini, P.
Bakers yeast uridine nucleosidase. Purification, composition, and physical and enzymatic properties
J. Biol. Chem.
250
9-13
1975
Saccharomyces cerevisiae
brenda
Raggi-Ranieri, M.; Ipata, P.L.
Partial purification and properties of uridine nucleosidase from Baker's yeast
Ital. J. Biochem.
20
27-43
1971
Saccharomyces cerevisiae
brenda
Achar, B.S.; Vaidyanathan, C.S.
Purification and properties of uridine hydrolase from mung-bean (Phaseolus radiatus) seedlings
Arch. Biochem. Biophys.
119
356-362
1967
Vigna radiata var. radiata
brenda
Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.
New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism
Curr. Genet.
36
130-136
1999
Saccharomyces cerevisiae
brenda
Mitterbauer, R.; Karl, T.; Adam, G.
Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase
Appl. Environ. Microbiol.
68
1336-1343
2002
Saccharomyces cerevisiae, Homo sapiens, Crithidia fasciculata (Q27546)
brenda
Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.
The URH1 uridine ribohydrolase of Saccharomyces cerevisiae
Curr. Genet.
41
132-141
2002
Saccharomyces cerevisiae (Q04179), Saccharomyces cerevisiae
brenda
Katahira, R.; Ashihara, H.
Dual function of pyrimidine metabolism in potato (Solanum tuberosum) plants: pyrimidine salvage and supply of beta-alanine to pantothenic acid synthesis
Physiol. Plant.
127
38-43
2006
Solanum tuberosum
brenda
Belenky, P.; Christensen, K.C.; Gazzaniga, F.; Pletnev, A.A.; Brenner, C.
Nicotinamide riboside and nicotinic acid riboside salvage in fungi and mammals. Quantitative basis for Urh1 and purine nucleoside phosphorylase function in NAD+ metabolism
J. Biol. Chem.
284
158-164
2009
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
brenda
Jung, B.; Floerchinger, M.; Kunz, H.H.; Traub, M.; Wartenberg, R.; Jeblick, W.; Neuhaus, H.E.; Moehlmann, T.
Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis
Plant Cell
21
876-891
2009
Oryza sativa, Arabidopsis thaliana (Q9SJM7)
brenda
Mach, J.
Uridine ribohydrolase and the balance between nucleotide degradation and salvage
Plant Cell
21
699
2009
Arabidopsis thaliana (Q9SJM7)
brenda
Jung, B.; Hoffmann, C.; Moehlmann, T.
Arabidopsis nucleoside hydrolases involved in intracellular and extracellular degradation of purines
Plant J.
65
703-711
2011
Arabidopsis thaliana
brenda
Arivett, B.; Farone, M.; Masiragani, R.; Burden, A.; Judge, S.; Osinloye, A.; Minici, C.; Degano, M.; Robinson, M.; Kline, P.
Characterization of inosine-uridine nucleoside hydrolase (RihC) from Escherichia coli
Biochim. Biophys. Acta
1844
656-662
2014
Escherichia coli
brenda
Shea, T.A.; Burburan, P.J.; Matubia, V.N.; Ramcharan, S.S.; Rosario, I.; Parkin, D.W.; Stockman, B.J.
Identification of proton-pump inhibitor drugs that inhibit Trichomonas vaginalis uridine nucleoside ribohydrolase
Bioorg. Med. Chem. Lett.
24
1080-1084
2014
Trichomonas vaginalis
brenda
Kopecna, M.; Blaschke, H.; Kopecny, D.; Vigouroux, A.; Koncitikova, R.; Novak, O.; Kotland, O.; Strnad, M.; Morera, S.; von Schwartzenberg, K.
Structure and function of nucleoside hydrolases from Physcomitrella patens and maize catalyzing the hydrolysis of purine, pyrimidine, and cytokinin ribosides
Plant Physiol.
163
1568-1583
2013
Physcomitrium patens, Zea mays, Zea mays (H9D3U7)
brenda
Baccolini, C.; Witte, C.P.
AMP and GMP catabolism in Arabidopsis converge on xanthosine, which is degraded by a nucleoside hydrolase heterocomplex
Plant Cell
31
734-751
2019
Arabidopsis thaliana (Q9SJM7), Arabidopsis thaliana
brenda