Protein Variants | Comment | Organism |
---|---|---|
W54L | less active than the wild-type enzyme with ethanol, 1-propanol and 1-butanol. With 1-pentanol and 1-hexanol the mutant enzyme is a better catalyst than the wild-type enzyme | Equus caballus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
cinnamyl alcohol | mutant enzyme W54L | Equus caballus | |
4 | - |
ethanol | wild-type enzyme Adh1 | Equus caballus | |
4.58 | - |
cinnamyl alcohol | wilde-type enzyme ADh1 | Equus caballus | |
6.5 | - |
Hexanol | mutant enzyme W54L | Equus caballus | |
6.7 | - |
4-Methyl-1-pentanol | mutant enzyme W54L | Equus caballus | |
11.8 | - |
Pentanol | mutant enzyme W54L | Equus caballus | |
13.4 | - |
ethanol | mutant enzyme W54L | Equus caballus | |
13.6 | - |
Propanol | wilde-type enzyme ADh1 | Equus caballus | |
13.8 | - |
Hexanol | wilde-type enzyme ADh1 | Equus caballus | |
29.4 | - |
butanol | mutant enzyme W54L | Equus caballus | |
43.9 | - |
Propanol | mutant enzyme W54L | Equus caballus | |
53.8 | - |
4-Methyl-1-pentanol | wilde-type enzyme ADh1 | Equus caballus | |
53.9 | - |
butanol | wilde-type enzyme ADh1 | Equus caballus | |
56.2 | - |
Pentanol | wilde-type enzyme ADh1 | Equus caballus | |
93.3 | - |
butan-2-ol | wilde-type enzyme ADh1 | Equus caballus | |
193 | - |
propan-2-ol | mutant enzyme W54L | Equus caballus | |
268 | - |
2-propanol | wilde-type enzyme ADh1 | Equus caballus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Equus caballus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Equus caballus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Equus caballus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-1-butanol + NAD+ | - |
Equus caballus | ? + NADH | - |
? | |
4-methyl-1-pentanol + NAD+ | - |
Equus caballus | 4-methyl-1-pentanal + NADH | - |
? | |
butan-2-ol + NAD+ | - |
Equus caballus | butan-2-one + NADH | - |
? | |
cinnamyl alcohol + NAD+ | - |
Equus caballus | cinnamaldehyde + NADH | - |
? | |
ethanol + NAD+ | - |
Equus caballus | acetaldehyde + NADH | - |
? | |
hexanol + NAD+ | - |
Equus caballus | n-hexanal + NADH | - |
? | |
pentanal + NAD+ | - |
Equus caballus | pentanone + NADH | - |
? | |
propan-2-ol + NAD+ | - |
Equus caballus | acetone + NADH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
3-methyl-1-butanol | mutant enzyme W54L | Equus caballus | |
0.27 | - |
3-methyl-1-butanol | wild-type enzyme Adh 1 | Equus caballus | |
0.31 | - |
butan-2-ol | mutant enzyme W54L | Equus caballus | |
5.8 | - |
propan-2-ol | mutant enzyme W54L | Equus caballus | |
6.7 | - |
4-Methyl-1-pentanol | wild-type enzyme Adh 1 | Equus caballus | |
11.9 | - |
butan-2-ol | wild-type enzyme Adh 1 | Equus caballus | |
18.4 | - |
Pentanol | mutant enzyme W54L | Equus caballus | |
18.6 | - |
4-Methyl-1-pentanol | mutant enzyme W54L | Equus caballus | |
23.9 | - |
Hexanol | mutant enzyme W54L | Equus caballus | |
28.7 | - |
Propanol | mutant enzyme W54L | Equus caballus | |
29.6 | - |
Hexanol | wild-type enzyme Adh 1 | Equus caballus | |
32.7 | - |
butanol | mutant enzyme W54L | Equus caballus | |
53.1 | - |
propan-2-ol | wild-type enzyme Adh 1 | Equus caballus | |
56.2 | - |
Pentanol | wild-type enzyme Adh 1 | Equus caballus | |
98 | - |
cinnamyl alcohol | mutant enzyme W54L | Equus caballus | |
102 | - |
ethanol | mutant enzyme W54L | Equus caballus | |
118 | - |
butanol | wild-type enzyme Adh 1 | Equus caballus | |
123 | - |
ethanol | wild-type enzyme Adh 1 | Equus caballus | |
133 | - |
cinnamyl alcohol | wild-type enzyme Adh 1 | Equus caballus | |
308 | - |
ethanol | wild-type enzyme Adh 1 | Equus caballus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Equus caballus | |
NADH | - |
Equus caballus |