Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 201% relative activity at 10% (v/v) | Thermus sp. | |
Urea | 130% relative activity at 1 M | Thermus sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Thermus sp. |
General Stability | Organism |
---|---|
the presence of a second phase of a water-insoluble solvent like hexane or octane has only minor effects on the enzyme, which retains 80% of its activity, allowing the use of these solvents in aqueous/organic mixtures to increase the availability of low-water soluble substrates | Thermus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | 91% relative activity at 10 mM | Thermus sp. | |
dithiothreitol | 14% relative activity at 10 mM | Thermus sp. | |
EDTA | 2.3% relative activity at 10 mM | Thermus sp. | |
SDS | 0.4% relative activity at 10% (v/v) | Thermus sp. | |
Tween 80 | 13% relative activity at 10% (v/v) | Thermus sp. | |
Urea | 1% relative activity at 5 M | Thermus sp. | |
Zn2+ | 91% relative activity at 10 mM | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | in 200 mM bicine, pH 6.0, at 60°C | Thermus sp. | |
0.13 | - |
NAD+ | in 200 mM bicine, pH 9.0, at 60°C | Thermus sp. | |
2.09 | - |
Cyclohexanol | in 200 mM bicine, pH 9.0, at 60°C | Thermus sp. | |
3.68 | - |
cyclohexanone | in 200 mM bicine, pH 6.0, at 60°C | Thermus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not stimulated by 10 mM Li+, Mg2+, Co2+, and Ca2+ | Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37200 | - |
calculated molecular weight | Thermus sp. |
40000 | - |
SDS-PAGE | Thermus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus sp. | TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones | ? | - |
? | |
additional information | Thermus sp. ATN1 | TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | B2ZRE3 | - |
- |
Thermus sp. ATN1 | B2ZRE3 | - |
- |
Purification (Comment) | Organism |
---|---|
ultracentrifugation and Sepabeads EB-QA405 chromatography | Thermus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
crude enzyme, after heat treatment at 75°C for 20 min | Thermus sp. |
20.6 | - |
after 1.4fold purifictaion | Thermus sp. |
Storage Stability | Organism |
---|---|
-20°C, several months, no loss of activity | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-carvone + NADH + H+ | 1% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
(1S,3S)-3-methylcyclohexanol + NAD+ | 125% activity compared to cyclohexanol | Thermus sp. | (rac)-3-methylcyclohexanone + NADH + H+ | 163% activity compared to cyclohexanone | r | |
(R)-3-methylcyclohexanone + NADH + H+ | 2% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
(S)-1-phenyl-2-propanol + NAD+ | - |
Thermus sp. | phenylacetone + NADH + H+ | 9% activity compared to cyclohexanone | r | |
(S)-4-phenylbutan-2-ol + NAD+ | - |
Thermus sp. | benzylacetone + NADH + H+ | 3% activity compared to cyclohexanone | r | |
(S)-heptan-2-ol + NAD+ | - |
Thermus sp. | 2-heptanone + NADH + H+ | - |
r | |
(S)-pentan-2-ol + NAD+ | - |
Thermus sp. | 2-pentanone + NADH + H+ | - |
r | |
(S)-perillylalcohol + NAD+ | 52% activity compared to cyclohexanol | Thermus sp. | (S)-perillaldehyde + NADH + H+ | - |
? | |
1-(p-tolyl)-ethanol + NAD+ | 19% activity compared to cyclohexanol | Thermus sp. | 1-(4-methylphenyl)ethanone + NADH + H+ | 26% activity compared to cyclohexanone | ? | |
1-butanol + NAD+ | 142% activity compared to cyclohexanol | Thermus sp. | butanal + NADH + H+ | - |
r | |
1-butanol + NAD+ | 142% activity compared to cyclohexanol | Thermus sp. ATN1 | butanal + NADH + H+ | - |
r | |
1-heptanol + NAD+ | 80% activity compared to cyclohexanol | Thermus sp. | heptanal + NADH + H+ | - |
r | |
1-heptanol + NAD+ | 80% activity compared to cyclohexanol | Thermus sp. ATN1 | heptanal + NADH + H+ | - |
r | |
1-hexanol + NAD+ | 109% activity compared to cyclohexanol | Thermus sp. | hexanal + NADH + H+ | - |
r | |
1-indanol + NAD+ | - |
Thermus sp. | 1-indanone + NADH + H+ | 1% activity compared to cyclohexanone | r | |
1-octanol + NAD+ | 57% activity compared to cyclohexanol | Thermus sp. | octanal + NADH + H+ | - |
r | |
1-pentanol + NAD+ | 121% activity compared to cyclohexanol | Thermus sp. | pentanal + NADH + H+ | - |
r | |
1-phenyl-1,2-ethanediol + NAD+ | 1% activity compared to cyclohexanol | Thermus sp. | 1-phenyl-2-propanone + NADH + H+ | - |
r | |
1-phenyl-2-propanol + NAD+ | 3% activity compared to cyclohexanol | Thermus sp. | 1-phenyl-2-propanone + NADH + H+ | - |
r | |
1-phenylethanol + NAD+ | 3% activity compared to cyclohexanol | Thermus sp. | 1-phenylethanone + NADH + H+ | - |
r | |
1-phenylethanol + NAD+ | - |
Thermus sp. | acetophenone + NADH + H+ | 1% activity compared to cyclohexanone | ? | |
1-propanol + NAD+ | 153% activity compared to cyclohexanol | Thermus sp. | propanal + NADH + H+ | - |
r | |
2,4-pentanediol + NAD+ | 4% activity compared to cyclohexanol | Thermus sp. | ? | - |
? | |
2-butanol + NAD+ | 39% activity compared to cyclohexanol | Thermus sp. | 2-butanone + NADH + H+ | 4% activity compared to cyclohexanone | r | |
2-chlorocyclohexanone + NADH + H+ | 3% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
2-decalone + NADH + H+ | 28% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
2-heptanol + NAD+ | 63% activity compared to cyclohexanol | Thermus sp. | 2-heptanone + NADH + H+ | 5% activity compared to cyclohexanone | r | |
2-hexanol + NAD+ | 64% activity compared to cyclohexanol | Thermus sp. | 2-hexanone + NADH + H+ | 4% activity compared to cyclohexanone | r | |
2-mercaptoethanol + NAD+ | 11% activity compared to cyclohexanol | Thermus sp. | ? | - |
? | |
2-methyl-2,4-pentanediol + NAD+ | 1% activity compared to cyclohexanol | Thermus sp. | ? | - |
? | |
2-methylcyclohexanone + NADH + H+ | 46% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
2-octanol + NAD+ | 43% activity compared to cyclohexanol | Thermus sp. | 2-octanone + NADH + H+ | 4% activity compared to cyclohexanone | r | |
2-pentanone + NADH + H+ | 6% activity compared to cyclohexanol | Thermus sp. | 2-pentanol + NAD+ | - |
r | |
2-phenylcyclohexanone + NADH + H+ | 2% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
2-phenylethanol + NAD+ | 57% activity compared to cyclohexanol | Thermus sp. | 2-phenylethanone + NADH + H+ | - |
r | |
2-propanol + NAD+ | 6% activity compared to cyclohexanol | Thermus sp. | 2-propanone + NADH + H+ | - |
r | |
3,3,5-trimethylcyclohexanone + NADH + H+ | 2% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
3,4-dimethylbenzyl alcohol + NAD+ | 45% activity compared to cyclohexanol | Thermus sp. | 3,4-dimethylbenzaldehyde + NADH + H+ | - |
r | |
3,4-dimethylbenzyl alcohol + NAD+ | 45% activity compared to cyclohexanol | Thermus sp. ATN1 | 3,4-dimethylbenzaldehyde + NADH + H+ | - |
r | |
3,5-dimethylcyclohexanol + NAD+ | 1% activity compared to cyclohexanol | Thermus sp. | 3,5-dimethylcyclohexanone + NADH + H+ | - |
r | |
3-aminobenzyl alcohol + NAD+ | 8% activity compared to cyclohexanol | Thermus sp. | 3-aminobenzaldehyde + NADH + H+ | - |
r | |
3-methyl-2-cyclohexenone + NADH + H+ | 1% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
3-methylbutanol + NAD+ | 133% activity compared to cyclohexanol | Thermus sp. | 3-methylbutanone + NADH + H+ | - |
r | |
3-methylphenylethyl alcohol + NAD+ | 64% activity compared to cyclohexanol | Thermus sp. | ? | - |
? | |
3-pentanol + NAD+ | 2% activity compared to cyclohexanol | Thermus sp. | 3-pentanone + NADH + H+ | 2% activity compared to cyclohexanone | r | |
3-penten-2-one + NADH + H+ | 2% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
4-ethylcyclohexanol + NAD+ | 60% activity compared to cyclohexanol | Thermus sp. | 4-ethylcyclohexanone + NADH + H+ | - |
r | |
4-ethylcyclohexanone + NADH + H+ | 22% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
4-methylcyclohexanol + NAD+ | 56% activity compared to cyclohexanol | Thermus sp. | 4-methylcyclohexanone + NADH + H+ | - |
r | |
4-methylcyclohexanone + NADH + H+ | 25% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
acetylacetone + NADH + H+ | 1% activity compared to cyclohexanone | Thermus sp. | 4-hydroxypentan-2-one + NAD+ | 1% activity compared to cyclohexanone | r | |
benzyl alcohol + NAD+ | - |
Thermus sp. | benzaldehyde + NADH + H+ | 178% activity compared to cyclohexanone | r | |
benzyl alcohol + NAD+ | 47% activity compared to cyclohexanol | Thermus sp. | benzaldehyde + NADH + H+ | 154% activity compared to cyclohexanone | r | |
butanol + NAD+ | - |
Thermus sp. | butyraldehyde + NADH + H+ | 359% activity compared to cyclohexanone | r | |
cyclohexanol + NAD+ | 100% activity | Thermus sp. | cyclohexanone + NADH + H+ | 100% activity | r | |
cyclopentanone + NADH + H+ | 1% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? | |
decahydro-2-naphthol + NAD+ | 37% activity compared to cyclohexanol | Thermus sp. | ? | - |
? | |
ethanol + NAD+ | 88% activity compared to cyclohexanol | Thermus sp. | acetaldehyde + NADH + H+ | - |
r | |
isopropanol + NAD+ | - |
Thermus sp. | 2-propanone + NADH + H+ | - |
r | |
additional information | TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones | Thermus sp. | ? | - |
? | |
additional information | ADH exhibits a clear preference for primary alcohols and corresponding aldehydes for aliphatic substrates, in the oxidative direction activity steeply increases with chain length until 1-propanol and then decreases slightly again with growing chain length, alpha,beta-unsaturated ketones like 3-penten-2-one and cyclohexenone are not converted by ADH, almost no conversion of methanol (0.2%) and (+)-carvone (0.4%) is detected | Thermus sp. | ? | - |
? | |
additional information | TADH is a NAD(H)-dependent enzyme and shows a very broad substrate spectrum producing exclusively the (S)-enantiomer in high enantiomeric excess (more than 99%) during asymmetric reduction of ketones | Thermus sp. ATN1 | ? | - |
? | |
additional information | ADH exhibits a clear preference for primary alcohols and corresponding aldehydes for aliphatic substrates, in the oxidative direction activity steeply increases with chain length until 1-propanol and then decreases slightly again with growing chain length, alpha,beta-unsaturated ketones like 3-penten-2-one and cyclohexenone are not converted by ADH, almost no conversion of methanol (0.2%) and (+)-carvone (0.4%) is detected | Thermus sp. ATN1 | ? | - |
? | |
octanol + NAD+ | - |
Thermus sp. | octanal + NADH + H+ | 178% activity compared to cyclohexanone | r | |
pentanol + NAD+ | - |
Thermus sp. | valeraldehyde + NADH + H+ | 240% activity compared to cyclohexanone | r | |
propan-2-ol + NAD+ | - |
Thermus sp. | acetone + NADH + H+ | 6% activity compared to cyclohexanone | r | |
tetrahydro-4H-pyran-4-one + NADH + H+ | 10% activity compared to cyclohexanone | Thermus sp. | ? + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
the optimal pH for reduction is at a pH of 6.0 | Thermus sp. |
9 | - |
the optimal pH for oxidation is at a pH of 9.0 | Thermus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Thermus sp. |