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Literature summary for 1.1.1.179 extracted from
- Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui (2004), J. Bacteriol., 186, 6198-6207.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Haloarcula marismortui |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-xylose | pH 8.3, 37°C | Haloarcula marismortui |  |
| 2.3 | - |
D-ribose | pH 8.3, 37°C | Haloarcula marismortui | |
| 64 | - |
D-glucose | pH 8.3, 37°C | Haloarcula marismortui | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | maximal activity at 1.5 M | Haloarcula marismortui | |
| MgCl2 | maximal activity at 100 M | Haloarcula marismortui | |
| NaCl | maximal activity at 1.5 M | Haloarcula marismortui | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39900 | - |
4 * 39900, SDS-PAGE | Haloarcula marismortui |
| 175000 | - |
gel filtration | Haloarcula marismortui |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NADP+ | Haloarcula marismortui | initial step in xylose degradation | D-xylonolactone + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloarcula marismortui | Q5UY95 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloarcula marismortui |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:xylose-grown cell | - |
Haloarcula marismortui | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 100 | - |
pH 8.8, 37°C | Haloarcula marismortui |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + NADP+ | reaction is catalyzed with 70fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP* | Haloarcula marismortui | D-gluconolactone + NADPH + H+ | - |
? | |
| D-ribose + NADP+ | reaction is catalyzed with 1.8fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP* | Haloarcula marismortui | D-ribonolactone + NADPH + H+ | - |
? | |
| D-xylose + NAD+ | Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ | Haloarcula marismortui | D-xylonolactone + NADH + H+ | - |
? | |
| D-xylose + NADP+ | initial step in xylose degradation | Haloarcula marismortui | D-xylonolactone + NADPH + H+ | - |
? | |
| D-xylose + NADP+ | Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ | Haloarcula marismortui | D-xylonolactone + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 39900, SDS-PAGE | Haloarcula marismortui |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Haloarcula marismortui |
| 50 | - |
- |
Haloarcula marismortui |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | activity increases exponentially with temperature between 25°C and 45°C. 50°C: optimum | Haloarcula marismortui |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 273 | - |
D-glucose | pH 8.3, 37°C | Haloarcula marismortui | |
| 356 | - |
D-xylose | pH 8.3, 37°C | Haloarcula marismortui | |
| 360 | - |
D-ribose | pH 8.3, 37°C | Haloarcula marismortui | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Haloarcula marismortui |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | more than 50% of maximal activity | Haloarcula marismortui |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ | Haloarcula marismortui | |
| NADP+ | preferred electron acceptor. Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ | Haloarcula marismortui | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
D-glucose | pH 8.3, 37°C | Haloarcula marismortui | |
| 1.57 | - |
D-ribose | pH 8.3, 37°C | Haloarcula marismortui | |
| 2.97 | - |
D-xylose | pH 8.3, 37°C | Haloarcula marismortui | |
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Release 2023.1 (January 2023)
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